مقایسه پروفایل متابولیت‌های اولیه گیاه ‏Saueda salsa‏ تحت شرایط ‏مختلف تنش شوری

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی دکتری بیوتکنولوژی کشاورزی، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده علوم کشاورزی، دانشگاه زنجان، زنجان، ایران

2 استادیار گروه اصلاح نباتات و بیوتکنولوژی، دانشکده علوم کشاورزی، دانشگاه زنجان، زنجان، ایران

3 بخش فیزیولوژی مولکولی، پژوهشگاه بیوتکنولوژی و کشاورزی. کرج، ایران

4 استادیار بخش زیست شناسی سامانه ها، پژوهشگاه بیوتکنولوژی و کشاورزی کرج، کرج، ایران

چکیده

Suaeda salsa یک شبه هالوفیت یکساله با ارزش غذایی و تحمل شوری بالا بوده که به عنوان یک گیاه روغنی، دارویی و خوراکی بسیار حائز اهمیت می‌باشد. در حال حاضر مطالعات محدودی در زمینه بررسی تنوع متابولیکی در گیاه S. salsa وجود دارد. در این مطالعه برای درک شاخص تحمل به شوری با استفاده از بررسی تنوع متابولیکی‌ پروفایل اسیدهای آمینه‌ در گیاه S. salsa در غلظت‌های 0، 200 و 800 میلی مولار NaCl مورد ارزیابی قرار گرفت. نتایج بررسی فیزیولوژیک نشان داد، شوری به صورت معنی‌داری، محتوای سدیم (Na+) را در اندام‌های هوایی گیاه تحت تأثیر قرار می‌دهد، به طوریکه میزان آن در اندام‌های هوایی گیاه S. salsa در مقایسه با شاهد افزایش معنی‌داری نشان داد. علاوه بر این تجزیه به مولفه‌های اصلی نتایج نشان داد که تفاوت در تنوع متابولیکی قادر است 96 درصد از تغییرات فنوتیپی در گیاه S. salsa را تحت شوری توجیه نماید. مقایسه پروفایل اسیدهای آمینه در سطوح مختلف شوری نشان داد پرولین، متیونین، سترولین و لیزین بیشترین تجمع را تحت تنش 800 میلی مولار دارا می‌باشند. با توجه به نقش مهم این اسیدهای آمینه در کنترل تنش شوری مطالعات بیشتر برای کشف مکانیزم‌های سازگاری تحمل به شوری در گیاه S. salsa ضروری است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Comparative Profiles of Primary Metabolites of Suaeda salsa under Different Salt Stress Conditions

نویسندگان [English]

  • Shima Jamalirad 1
  • Mohammad Reza Azimi 2
  • Nayer Azam KhoshKholgh Sima 3
  • Mohammad Reza Ghaffari 4
1 Ph.D. Student, Department of Plant Breeding and Biotechnology (PBB), Faculty of Agriculture, Zanjan University, Zanjan, Iran
2 Assistant Professor, Department of Plant Breeding and Biotechnology (PBB), Faculty of Agriculture, Zanjan University, Zanjan, Iran
3 Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran. (ABRII), Karaj, Iran
4 Assistant Professor, Department of System Biology, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
چکیده [English]

Suaeda salsa is an annual halophyte with nutritional value and high salt tolerance, making it crucial as an oil, medicinal, and edible plant. Currently, there is limited research in the field investigating metabolic diversity in S. salsa. In this study, our aim was to understand the salinity tolerance mechanism by examining metabolic diversity, specifically the amino acids profile, in S. salsa exposed to 0 mM, 200 mM, and 800 mM NaCl. The results of the physiological study indicated that salinity significantly affected the sodium (Na+) content in the aerial parts of the plant, with a significant increase compared to the control. Principal component analysis (PCA) revealed that differences in metabolic diversity can explain 96% of the phenotypic variation in S. salsa under salinity stress. Comparison of amino acids profiles at different salinity levels showed the highest accumulation of proline, methionine, citrulline, and lysine under 800 mM salt stress. Given the crucial role of these amino acids in S. Salsa,further studies are necessary to uncover the mechanisms behind the adaptation response.

کلیدواژه‌ها [English]

  • Amino acids profile
  • Salinity stress
  • Suaeda salsa
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