نوع مقاله : علمی پژوهشی
نویسندگان
1
گروه ژنتیک و تولید گیاهی، دانشگاه کردستان، سنندج، ایران.
2
دانشگاه کردستان گروه ژنتیک و تولید گیاهی، دانشگاه کردستان، سنندج، ایران
3
پیام نورگروه کشاورزی، دانشگاه پیام نور، تهران، ایران
4
گروه گیاهپزشکی، دانشگاه کردستان، سنندج، ایران
چکیده
یکی از مضرترین آفات جنگلهای زاگرس، پروانهی جوانهخوار بلوط Tortrix viridana (Lep. Tortricidae) است. تنوع ژنتیکی جمعیتهای گیاه میزبان پروانهی جوانهخوار بلوط در جنگلهای بلوط شمال غرب ایران و منطقهی زاگرس شمالی با استفاده از توالی ژن 28s بررسی شد. جمعآوری نمونهها، در مناطق جنگلی استانهای آذربایجان غربی، لرستان، کردستان و کرمانشاه صورت گرفت. نمونههای جمعآوری شده که در مرحلهی لاروی بودند تا زمانی که به شفیره و سپس به حشرهی کامل تبدیل شوند، در آزمایشگاه نگهداری شدند. استخراج DNA به روش CTAB انجام و به منظور تکثیر ناحیه 28s از توالی ژن 28s جنس Tortrix از NCBI برای طراحی پرایمر استفاده شد. ناحیهی مورد نظر با استفاده از روش PCR تکثیر و محصولات PCR توالییابی شدند. 21 نمونه برای بررسی تنوع ژنتیکی با استفاده از ژن 28s انتخاب شد که 18 نمونه با کیفیت بالاتر توالی DNA برای بررسیهای بیشتر مورد استفاده قرار گرفت. توالیهای DNA با استفاده از نرم افزار Bioedit ویرایش و با نرم افزار MegaX تراز شد و درخت فیلوژنتیک به روش UPGMA با 1000 تکرار نمونهبرداری ترسیم گردید. ارزیابی ساختار ژنتیکی جمعیتها نشان داد که تنوع مابین جمعیتها بیشتر از درون جمعیتها است. نتایج درخت فیلوژنتیک نیز نشان داد که نمونههای مختلف پروانهی جوانهخوار بلوط بر اساس فاصلهی جغرافیایی دارای تنوع ژنتیکی هستند. بنابراین زمان ظهور آفت، رفتار و نوع کنترل و مدیریت آفت آنها هم متفاوت است.
کلیدواژهها
موضوعات
عنوان مقاله [English]
Genetic diversity of Tortrix viridana L. (Lep. Tortricidae) in Zagros Oak forests using 28s gene
نویسندگان [English]
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Rezvan Mousivand
1
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Mohammad Majdi
2
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ّFoad Fatehi
3
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Hamed ghobari
4
1
Department of Plant Production and Genetics, University of Kurdistan, Sanandaj Iran.
2
University of KurDepartment of plant production and Genetics, University of Kurdistan, Sanandaj Irandistan
3
Department of Agriculture, Payame Noor University, Tehran, Iran
4
Department of plant Protection, University of Kurdistan, Sanandaj Iran
چکیده [English]
One of the most harmful pests of Zagros forests is the Tortrix viridana (Lep. Tortricidae). Genetic diversity of Tortrix viridana host plant populations in the oak forests of northwestern Iran and the northern Zagros region was investigated using 28s gene sequence. The samples were collected from the forest areas of west Azarbaijan, Lorestan, Kurdistan and Kermanshah provinces. They were in the larval stage, were kept in laboratory conditions until they turned into pupa and then into a complete insect. DNA extraction was done by CTAB method. Also, in order to amplify the 28s region, the 28s gene sequence of Tortrix genus was used from NCBI for primer design. The desired region was amplified using the PCR method and the PCR products were sequenced. 21 samples were selected to investigate genetic diversity using the 28s gene, and 18 sequences DNAs were of suitable quality for further investigations. The DNA sequences were edited using Bioedit software and aligned using MegaX software, and the phylogenetic tree was drawn by UPGMA method with 1000 sampling repetitions. The evaluation of the genetic structure of populations showed that the diversity between populations is greater than within populations. The results of the phylogenetic tree also showed that different samples of the Tortrix viridana have genetic diversity based on geographical distance. Therefore, the time of appearance of the pest, their behavior and their type of control and management are also different.
کلیدواژهها [English]
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Tortrix viridana
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Pest
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geographical distance
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28s rDNA
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