ارزیابی تنوع ژنتیکی آفتابگردان آجیلی (Helianthus annuus L.) با استفاده از نشانگرهای ریزماهواره

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد بیوتکنولوژی کشاورزی، پژوهشکده زیست‌فناوری دانشگاه ارومیه،

2 دانشیار گروه اصلاح و بیوتکنولوژی گیاهی دانشکده کشاورزی دانشگاه ارومیه،

3 دانشیار گروه بیوتکنولوژی کشاورزی، دانشگاه پیام نور، تهران، ایران.

چکیده

تنوع ژنتیکی بین و درون 50 توده آفتابگردان آجیلی  با استفاده از 10 نشانگر ریزماهواره مورد ارزیابی قرار گرفت. تعداد آلل در مکان‌های ژنی بین 2 تا 3 با میانگین 1/2 آلل به ازای هر مکان­ژنی بود. تعداد آلل مؤثر بین 948/1 در مکان­ژنی ORS785 و 468/1 در مکان­ژنی ORS1088 با میانگین 825/1 آلل برآورد شد. بیشترین میزان هتروزیگوسیتی مورد انتظار (484/0) در مکان­­ژنی ORS785 و کمترین آن (268/0) در مکان­ژنی ORS1088 برآورد شد. میانگین هتروزیگوسیتی مورد انتظار و مشاهده شده به ترتیب برابر 435/0 و 722/0 بود. محتوای اطلاعات چند شکلی (PIC) بین 573/0 در مکان­­ژنی ORS785 و 334/0 در مکان­ژنی ORS1088 با میانگین 503/0 برآورد گردید. تجزیه به مختصات اصلی (PCoA) نشان داد دو مؤلفه اصلی اول مجموعا 71/31% از واریانس کل را توجیه می نمایند. گروه­بندی توده­های آفتابگردان آجیلی مورد مطالعه براساس ضریب فاصله ژنتیکی نی و با استفاده از الگوریتم Complete، آن­ها را در 3 گروه مجزا قرار داد. تجزیه واریانس مولکولی نشان داد تنوع درون توده­ها بیشتر از تنوع بین تودها می­باشد. بنابراین در برنامه­های به‌نژادی آفتابگردان آجیلی، می­توان انتخاب را درون توده­ها انجام داد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Studying genetic diversity in confectionary sunflower (Helianthus annuus L.) by using microsatellit markers

نویسندگان [English]

  • Marjan Jannatdoust 1
  • Reza Darvishzadeh 2
  • Mohammad Ali Ebrahimi 3
1 Institute of Biotechnology, Urmia University, Urmia, Iran
2 Associate Professor, Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran,
3 Associate Professor, Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran.
چکیده [English]

In this study, genetic variability among and within 50 confectionery sunflower populations was investigated using 10 microsatellite markers (SSR). The number of alleles per locus ranged from 2 to 3 with an average of 2.1. The number of effective alleles per locus was estimated from 1.948 (locus ORS785) to 1.468 (locus ORS1088) with an average of 1.825. The highest expected heterozygosity (0.484) was estimated for ORS785 locus and the lowest one (0.268) estimated for ORS1088. The mean of expected and observed heterozygosity was 0.435 and 0.722, respectively. Polymorphic information content varied between 0.572 in ORS785 locus and 0.334 in ORS1088 with an average of 0.503. A principal co-ordinates analysis (PCoA) showed that the first two Eigen-values explained 31.71% of the cumulative variation. Cluster analysis based on complete algorithm and Nei genetic distance grouped the studied populations in 3 main classes. Analysis of molecular variance revealed that the high part of total variation was due to within populations. So it will be better to do selection within populations in breeding programs.

کلیدواژه‌ها [English]

  • sunflower
  • analysis of molecular variance
  • Cluster analysis
  • Polymorphic information content
  • Molecular markers

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