شناسایی و جداسازی رونوشت‌های پاسخگو به پتاسیم کلراید از گیاه آلوروپوس لیتورالیس

نوع مقاله : علمی پژوهشی

نویسندگان

1 کارشناس‌ارشد اصلاح‌نباتات، پژوهشکده ژنتیک و زیست‌فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری.

2 استاد پژوهشکده ژنتیک و زیست‌فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری.

3 استادیار گروه بیوتکنولوژی کشاورزی، دانشکده مهندسی انرژی و فناوری‌های نوین، دانشگاه شهید بهشتی، تهران.

چکیده

شوری خاک با ایجاد تنش اسمزی و بر هم زدن تعادل یونی تولیدات گیاهی را محدود می­کند و منجر به خسارت در سطح مولکولی و نهایتاً مرگ سلول می­گردد. در این تحقیق آنالیز بیان ژن مبتنی بر تکنیک cDNA-AFLP جهت مقایسه پروفایل بیانی ناشی از تنش شوری KCl در سه سطح 0 (شاهد)، 200 و 400 میلی مولار در گیاه آلوروپوس لیتورالیس که نزدیک­ترین خانواده به غلات می­باشد، انجام شد. از میان 34 عدد EST  جداسازی شده، 27 عدد EST با طول میانگین 280 جفت باز بدست آمد. در این میان 80% از رونوشت­ها با پروتئین­ها و توالی­های اسید نوکلئیک شناسایی شده در ارگانیسم­های دیگر، شباهت نشان دادند. از طرفی دیگر، 6 رونوشت به عنوان ژن­های جدید در نظر گرفته شدند. در نهایت 27 عدد EST  در بانک ژن به ثبت رسید که مهمترین آنها شامل گروه­های پروتئینی سینتاکسین، آدنوزیل متیونین دکربوکسیلاز و پروتئین­های ریبوزومی می‌باشند. گروه­بندی EST­ها بر مبنای نوع پاسخ به تنش­ها، آنالیز عملکردی و خصوصیت یابی ژن­های پاسخگو در ریشه گیاه آلوروپوس لیتورالیس به تنش­ مذکور را در مطالعات آتی روی این گیاه که از اعضای خانواده غلات می‌باشد، تسهیل خواهد کرد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Identification and Isolation of Transcripts in Response to KCl from Aeluropus littoralis

نویسندگان [English]

  • Seyyedeh farzaneh Fatemi ardestani 1
  • Ghorbanali Nematzadeh 2
  • Hossein Askari 3
  • Hamidreza Hashemi 1
1 M.Sc. of Plant Breeding, Genetic and Agricultural Biotechnology Institute of Tabarestan, University of Agriculture and Natural Resources of Sari, Sari, Iran,
2 Professor of Biotechnology, Genetic and Agricultural Biotechnology Institute of Tabarestan, University of Agriculture and Natural Resources of Sari, Sari, Iran,
3 Assistant Professor, Department of Biotechnology, Faculty of New Technologies and Energy Engineering, Shahid Beheshti University, Tehran, Iran
چکیده [English]

Soil salinity limits crop production by creating osmotic stress and disruption of ion homeostasis, leads to damage at the molecular level and finally cell death. In this study, gene expression analysis based on cDNA-AFLP technique was used to compare the expression profiles of KCl stress at three levels: 0 (control), 200mM and 400mM, in Aeluropus littoralis which is the closest family to cereal. Among 34 isolated ESTs, 27 ESTs were obtained with the average length of 280 bp. The nucleotide sequences were compared with those in the GenBank database. Approximately 80% of the ESTs show homology to nucleotide or amino acid sequences in the GenBank database and 6 ESTs show no significant similarity in the GenBank database which considered as novel genes. Finally, 27 ESTs were recorded in NCBI database which are included potassium transporter, ribosomal protein, NADH dehydrogenase and golgin. The result of this research is very important to understand molecular basis and resistance mechanisms of drought stress for breeding and genetic engineering to improve crop resistance against stress and the production of resistant plants. EST classification based on responses to stress, will facilitate performance analysis, characterization of responsive genes in plant roots of Aeluropus littoralis to stress in future studies on this herb that is a member of the Poaceae family.

کلیدواژه‌ها [English]

  • Aeluropus littoralis
  • cDNA-AFLP
  • Gene expression
  • Salinity

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