نوع مقاله : علمی پژوهشی
نویسندگان
1 گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
2 گروه بیوتکنولوژی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Tea (Camellia sinensis) is native to East Asia, the Indian subcontinent and Southeast Asia, and its leaves are used as one of the most popular drinks in the world. Environmental stresses are one of the most important factors affecting the quantity and quality of agricultural and garden crops, including tea. One of the prominent effects of environmental stresses on plants is the increase in reactive oxygen species (ROS) production. Ascorbate peroxidase (APX) is a key antioxidant enzyme to inhibit ROS in plants. This study was conducted to identify, study the evolution, function of the APX gene family in tea. To identify APX homologous proteins in the tea genome, protein sequences of the APX family from several monocot and dicot plants were used. BlastP results identified 9 homologous sequences from the APX gene family on different tea scaffolds. Based on phylogenetic relationships, APX gene family proteins in tea and the studied plants, including Arabidopsis, rice, maize, and potato, were divided into four distinct evolutionary groups. Due to the relatively equal distribution of genes from monocot and dicot plants in phylogenetic groups, it seems that the evolution of these genes occurred from a common ancestral gene before the divergence of monocot and dicot plants. Investigation of the expression of APX homologous genes in different plant tissues and various environmental stresses showed that CsAPX1, CsAPX3, CsAPX4, CsAPX5, and CsAPX8 genes had moderate to high expression levels, indicating the key role of these genes in different growth stages and various abiotic stresses.
کلیدواژهها [English]