نوع مقاله : علمی پژوهشی
نویسندگان
1 گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه لرستان، خرمآباد، ایران
2 دانشگاه شیراز
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Plant diseases, especially fungal and oomycetes associated diseases are major challenges in modern agriculture in the world. Pathogen associated molecular pattern (PAMP) such as some fungi and oomycetes cell wall chitin, elicit or stimulate defence signalling, leading to expression of R-genes, production of reactive oxygen species (ROS) as well as production of a wide range of defence-related metabolites. Chitin elicitors lead to the expression of defence-related genes such as chitinases, consequently leading to pathogens’ cell wall chitin degradation. In order to evaluate the expression level of some chitinases in potato plants and the activity of antioxidant enzymes, leaves of a tolerant potato genotype (jelly) was challenged with chitin oligomers in vitro. Result of this study revealed that 48 hours post chitin induction, the expression of different classes of chitinase genes were significantly increased in comparison with that of control. Class I chitinase (Soltu.DM.10G017450) and class III chitinase (Soltu.DM.11G026160) genes, had the highest (5.5-fold relative to control) and the lowest (1.1-fold relative to control) expression level after 48 hours post chitin inoculation, respectively. Interestingly, the activity of antioxidant enzymes did not change, suggesting that exogenous chitin treatment did not induce signalling pathways involved in antioxidant enzymes biosynthesis. These results may imply that application of chitin does not induce the signaling pathways involved in the biosynthesis of antioxidant enzymes at 48 hours after chitin treatment. Furthermore, the results of the present study illustrated that chitinase genes can be cloned by genetic engineering approaches to generate transgenic plants with enhanced plant pathogens resistance.
کلیدواژهها [English]
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