نوع مقاله : علمی پژوهشی
نویسندگان
1 بیوتکنولوژی کشاورزی دانشگاه صنعتی شاهرود، شاهرود، ایران
2 گروه به نژادی و بیوتکنولوژی گیاهی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Pod distortion syndrome (PDS) is a particular type of growth in which soybean plants remain green long after pod maturation. Green stems even sometimes leaves and green petiole impose different problems at harvesting and lead to yield reduction. Protein expression profile of PDS soybean cultivar Katul compared with non-PDS soybean to investigate the molecular mechanisms of this syndrome. Therefore soybean leaf samples were collected at R6 vegetative stage from the field through surway sampling and protein extracted by phosphate buffer method.A total of 124 reproducible protein spots were distinguished by 2-dimentional electrophoresis through isi-electric focusing in the first and sds-PAGE in the second dimentions. Thirty four spots indicated by induction factor in which 11 protein spots indicated significant expression changes, statistically. Proteins interfering in PDS were classified into metabolism and energy production, protein destination and storage, signal transduction, ROS scavenging and detoxification categories. Glutamate dehydrogenase, malate dehydrogenase, methionine syntetase, stem 31 kDa glycoprotein, 14-3-3-like protein, germin like proteins, thiroredoxin and ond one protein with unknown function were identified through mass spectrometry in which all the proteins were shown decrease in expression change. The finding suggest that the key regulators of PDS in soybean plants are 14-3-3 and jermin like protein.
کلیدواژهها [English]
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