نوع مقاله : علمی پژوهشی
نویسندگان
1 کارشناسی ارشد بیوتکنولوژی و ژنتیک مولکولی
2 دکتری، دانشگاه شهید بهشتی تهران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
The chalcone synthetase (CHS) gene is a key gene in the flavonoid biosynthesis pathway in plants. This gene encodes the enzyme chalcone synthase, which plays a crucial role in the early stages of the flavonoid biosynthesis pathway. In addition to its primary role in
flavonoid biosynthesis, the CHS gene may also be involved in other metabolic pathways, such as the production of compounds that are precursors to cannabinoids (like THC and CBD). Due to the importance of this gene in Cannabis sativa, it was studied using various bioinformatics databases and software tools.
A search of the nucleotide sequence of accession NM_001397940 from Cannabis sativa in the NCBI database revealed 100 matches. The highest Max Score (805) belonged to the alignment with NP_001384869.1 from the Cannabis plant itself, with 100% coverage and 100% similarity. The lowest Max Score (746) corresponded to the alignment with QRV61377.1 from the species Litchi Chinensis with 99% coverage and 92.01% similarity. The E-Value in this section was also used to determine the significance of the matches. Protein sequence alignment with accession number NP_001384869.1 from Cannabis sativa in the NCBI database also showed 100 matches. Phylogenetic analysis of the top 20 species that showed homology with the CHS gene in the BLAST n analysis created a dendrogram, classifying the species into six distinct groups. Genetic diversity analysis using MEGA 6 software indicated that approximately 31% of the CHS gene sequence has undergone substitution mutations and only 8% of these regions contain informative polymorphisms.
کلیدواژهها [English]
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