نوع مقاله : علمی پژوهشی
نویسندگان
گروه ژنتیک و تولیدات گیاهی ، دانشکده کشاورزی، دانشگاه کردستان، سنندج، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Thymoquinone, the main compound of black cumin oil, has numerous therapeutic effects. 1-Deoxy-D-xylulose 5-phosphate reductase(DXR) and geranylgeranyl-diphosphate-synthase(GTS) are two key genes in the biosynthetic pathway of thymoquinone. In this study, the expression changes of genes DXR and GTS were investigated in two ecotypes of Iranian black cumin with the highest and lowest levels of thymoquinone, including ecotypeH(Semirum) and ecotypeK(Eqlid), as well as the effect of methyl jasmonate treatment on the expression of these genes. Sampling was conducted at 12, 24,and 48hours after the application of methyl jasmonate at a concentration of 100µM during the immature seed stage. Gene expression was analyzed using RT-qPCR. The results at a 5% significance level showed that the expression of both genes in ecotypeH is significantly higher than in ecotypeK. The expression level of the GTSgene in both ecotypes was influenced by methyl jasmonate, initially decreasing in the first 12hours, then gradually increasing and reaching a maximum at 48hours, which was significantly different from the control. However, the expression of the DXRgene varied between the two ecotypes: in ecotypeH, the expression of this gene significantly decreased, reaching its minimum at 48 hours, while in ecotypeK, it initially increased at 12hours and then decreased to a minimum at 48hours. Overall, these findings indicate that the gene expression of GTS and DXRgenes involved in the thymoquinone biosynthetic pathway in black cumin is genotype-dependent,and methyl jasmonate treatment can effectively induce the expression of these genes, depending on the genotype and time which consequently can increase the thymoquinone content
کلیدواژهها [English]
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