تأثیر هورمون‌های اسید جیبرلیک، اسید جاسمونیک و اسید سالیسیلیک بر بیان ژن فنیل‌آلانین‌آمونیالیاز (PAL) در مراحل مختلف رشد .Ocimum basilicum L

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی‌ارشد بیوتکنولوی کشاورزی، دانشگاه زابل، زابل،

2 دانشیار گروه بیوتکنولوژی کشاورزی، دانشگاه زابل، زابل

3 مربی پژوهشکده زیست فناوری دانشگاه زابل، زابل

چکیده

گیاه Ocimum basilicum L.  از خانواده نعناعیان است که دارای ترکیبات حلقوی و اسانس بوده و خواص ضد باکتریایی و آنتی‌اکسیدانی دارد، که سرشار از ترکیبات فنیل پروپانوئیدی است. آنزیم فنیل‌آلانین آمونیالیاز یکی از آنزیم­های کلیدی در مسیر تولید ترکیبات فنیل پروپانوئیدی می­باشد. شواهد نشان می­دهد که هورمون­های گیاهی بر بیان ژن در گیاهان مؤثر بوده و موجب افزایش تولید متابولیت­های ثانویه می‏گردند. علاوه بر این سیستم دفاعی گیاه را از طریق فعال‏سازی رونویسی ژن­های مرتبط با مکانیسم دفاعی گیاه تحریک نموده باعث تنظیم مقاومت القایی می­گردند. در این تحقیق، بیان ژن فنیل‌آلانین‌آمونیالیاز و فعالیت آنزیم فنیل‌آلانین‌آمونیالیاز در گیاه ریحان تحت تیمار هورمون­های اسید جیبرلیک، اسید جاسمونیک و اسید سالیسیلیک با غلظت 1/0 میلی‏مولار در لیتر با روش Real Time PCR در مراحل رشدی گیاهچه­ای، پیش­گلدهی و گلدهی مورد بررسی قرار گرفت. اختلاف سطوح بیان ژن با روش دانکن در سطح 05/0P≤ مقایسه و آنالیز­ها توسط نرم افزار SAS v9 صورت گرفت. نتایج نشان دهنده افزایش در میزان بیان ژن و فعالیت آنزیم فنیل‌آلانین‌آمونیالیاز در تیمار هورمون اسید جاسمونیک در مرحله گلدهی بود. بیان ژن فنیل‌آلانین‌آمونیالیاز بین تیمار هورمون­های اسید جیبرلیک، اسید جاسمونیک و اسید سالیسیلیک و نمونه شاهد در سطح احتمال 5 درصد معنا­دار بود. تیمار اسید جاسمونیک، اسید جیبرلیک و اسید سالیسیلیک باعث افزایش بیان ژن و فعالیت آنزیم فنیل‌آلانین‌آمونیالیاز شد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Different Growth Hormones on Gene Expression of Phenylalanine Ammonia Lyase (PAL) in Ocimum basilicum L.

نویسندگان [English]

  • Sara Abdekhani 1
  • Mahmood Solouki 2
  • Yasub Shiri 3
1 M.Sc. of Agricultural Biotechnology, University of Zabol, Zabol, Iran
2 Associate Professor, Department of Biotechnology, University of Zabol, Zabol, Iran
3 Lecturer, Biotechnology Research Institute of University of Zabol. Zabol, Iran.
چکیده [English]

Basil (Ocimum basilicum L.) is a member of a Lamiaceae family with aroma compounds and high essence. Furthermore, the essential oils of basil leaves are composed of phenylpropanoids, which have shown antibacterial and antioxidant properties. Phenylalanine ammonia Lyase is an important enzyme in phenylpropanoid pathway. The literature indicated that plant hormones affect gene expression in plants and increase the production of secondary metabolites. In addition, the hormones stimulate the immune system through transcriptional activation of defense related genes, and in turns, increase induced resistance of plants. In the current study, Real Time PCR was used to evaluate the gene expression and enzymatic activity of phenylalanine ammonia. The plants were treated with three different hormones including gibberellic acid, jasmonic acid, and salicylic acid with 0.1 mM/L concentration. The activities were studied during three different plant growth stages which included seedlings, pre-flowering stage, and flowering stage. The difference between gene expressions levels were analyzed by Duncan method (P≤0.05) using SAS software (version 9.0). During the flowering stage, the results showed an increase in gene expression of phenylalanine ammonia Lyase in plants treated with jasmonic acid. The gene expression of phenylalanine ammonia Lyase was significantly higher (5%) for all treated samples compared to the control samples. The studied hormones increased the gene expression and enzymatic activity of phenylalanine ammonia Lyase. 

کلیدواژه‌ها [English]

  • phenylalanine ammonia lyase
  • hormone
  • RT-PCR

مراجع

Ali MB, Hahn EJ, Paek KY (2007) Methyl jasmonate and salicylic acid induced oxidative stress and accumulation of phenolics in Panax ginseng bioreactor root suspension cultures. Molecules 12: 607-621.
Chakraborty M, Karun A, Mitra A (2009) Accumulation of Phenylpropanoid derivatives in chitosan-induced cell suspension culture of Cocos nucifera. Journal of Plant Physiology. 166: 63-71.
Chen C, Zheng Z, Huang J, Lai Z, Fan B (2006) Biosynthesis of salicylic acid in plants. Plant Signaling and Behavior 4: 493-496.
Dixon RA, Paiva NL (1995) Stress-lnduced Phenylpropanoid Metabolism. Plant Cell 7:1085-1097.
Gang DR, Lavid N, Zubieta C, Chen F, Beuerle T, Lewinsohn E, Noel J P, Pichersky E (2002) Characterization of phenylpropene O-methyltransferases from sweet basil: facile change of substrate specificity and convergent evolution within a plant O-methyltransferase family. Journal of Plant Cell 14: 505-519.
Gao-Bin P, Dong-Ming M, Jian-Lin C, Lan-Qing M, Hong W, Guo-Feng L, He-Chun Y, Ben-Ye L (2009) Salicylic acid activates artemisinin biosynthesis in Artemisia annua L. Plant Cell Report 28: 1127-1135.
Ghahreman A (1994) Cormophytes of Iran. Academic Publishing Center of Iran 89: 325-237. (In persion).
Javanmardi J, Khalighi A, Khashi A, Bais HP, Vivanco JM (2002) Chemical characterization of Basil (Ocimum basilicum L.) found in local accessions and used in traditional medicines in Iran. Agricultural and Food Chemistry 50: 5878-5883.
Kang SM, Jung HY, Kang YM, Yun DJ, Bahk JD, Yang JK, Choi MS (2004) Effects of methyl jasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitious root cultures of Scopoliaparviflora. Plant Science 166: 745-751.
Labra M, Milele M, Ledda B, Grassi F, Mazzei M, Sala F (2004) Morphologicalcharacterization essential oil composition and DNA genotyping of Ocimum basilicum L.cultivars. Journal of Plant Science 167: 725- 731.
Lewinsohn E, Ziv-Raz I, Dudai N, Tadmor Y, Lastochkin E, Larkov O, Chaimovitsh D, Ravid U, Putievsky E, Pichersky E, Shoham Y (2000) Biosynthesis of estragole and methyl- eugenol in sweet Basil (Ocimum basilicum L.) Developmental and chemotypic association of allylphenol O-methyltransferase activities. Plant Science 160: 27-35.
Moradi A, Sharifi M, Mousavi A (2010) Study on gene expression of Hyoscyamine 6-β hydroxylase (H6H) and Putrescine N-methyl transferase (PMT) isozymes under different concentrations of salicyclic acid in hairy roots and different organs of Atropa belladonna L. Journal of Biology 24(3): 366-372.
Popova L, Pancheva T, Uzonova A (1997) Salicylic acid: properties, biosynthesis and physiological role. Plant Physiology 23: 85-93.
Raskin I (1992) Salicylate, a new plant hormone. Plant Physiol. 99: 799-803.
Rodway SJ, Gates DW, Brindle C (1991) Control of early seedling growth in varietal lines of hexaplpid wheat (Triticum aestivum), durum wheat (Triticum durum), and barley (Hrdeum vulgare) in response to the phthalimide growth regulant, AC 94,377. Pant Growth Regulation 10: 243-259.
Sajjadi SE (2006) Analysis of the essential oils of two cultivated Basil (Ocimum basilicum L.) from Iran. Daru 14: 1-3.
Schaller F (2001) Enzymes of the biosynthesis of octadecanoid-derived signaling molecules. Journal Experimental Botany. 52: 11-23.
Simon JE, Morales MR, Phippen WB, Vieiram RF, Hao Z (1999) Basil a source of arom compounds and popular culinary and omamental herb. Plant Science 105: 499-505
Simon JE, Deshamps C (2002) Regulation of essential oil biosynthesis in basi (Ocimum basilicum L.). Plant Science 115: 741-746.
Shabani L, Ehsanpour AA (2009) Induction of antioxidant enzymes, phenolic and flavonoid compounds in in vitro culture of licorice (Glycyrrhiza glabra L.) using methyl jasmonate and salicylic acid. Iranian Journal of Plant Biology 21(3):421-432.
Sharan M, Taguchi G, Gonda K, Jouke T, Shimosaka M, Hayashida N, Okazaki M (1998) Effects of methyl jasmonate and elicitor on the activation of phenylalanine ammonialyase and the accumulation of scopoletin and scopolin in tobacco cell cultures. Plant Science 132: 13-19.
Staswick PE (1992) Jasmonate, genes, and fragrant signals. Plant Physiol 99: 892-807.
Tahsili J, Sharifi M, Behmanesh B, Pourbozorgi Rudsari N, Ziaei M (2010) Gene expression of eugenol O - methyl transferase and components of essential oils in (Ocimum basilicum L.) at different stages of growth. Journal of Biology 23 (1): 25-18.
Telci I, Bayram E, Yilmaz G, Avc B (2006) Variability in essential oil composition of Turkish basils (Ocimum basilicum L.). Journal of Biochemistry and System Ecology 34: 489-497.
Wang JW, Zheng LP, Wu JY, Tan RY (2006) Involvement of nitric oxide in oxidative burst, phenylalanine ammonia-lyase activation and Taxol production induced by low-energy ultrasound in Taxus yunnanensis cell suspension cultures. Nitric Oxide 15: 351-358.
Weathers PJ, Bunk G, Mccoy MC (1997) Effects of gibberellic acid on hairy root cultures of Artemisia annua: growth and artemisinin production. In Vitro Cellular & Developmental Biology. Plant 41: 47-53.
Werker E, Putievsky E, Ravid U, Dudai N, Katzir I (1993) Glandular h and essential oil in developing leaves of Ocimum basilicum L. (Lamiaceae).Ann. Botany 71: 43-50.
Yu KW, Gao W, Hahn EJ, Paek KY (2002) Jasmonic acid improves ginsenoside accumulation in adventitious root culture of Panax ginseng C.A. Meyer. Journal of Biochemistry 11: 211-215.
Ziaei M, Sharifi M, Behmanesh M, Razavi K (2012) Gene expression and activity of phenyl alanine ammonialyase and essential oil composition of Ocimum basilicum L. at different growth stage. Journal of Biotechnology 10: 32-39.
Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnology 23: 283-333.
 
 

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