کاربرد نشانگرهای ریزماهواره و برخی از صفات مورفولوژیک در شناسایی کلکسیون‌های هسته .Prunus dulcis Mill

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشیار گروه بیوتکنولوژی کشاورزی، دانشگاه‌‌ پیام نور، تهران،

2 دانش آموخته کارشناسی ارشد بیوتکنولوژی کشاوزی دانشگاه پیام نور

3 استادیار بخش ژنومیکس، پژوهشکده بیوتکنولوژی کشاورزی ایران، کرج

4 دانشیار بخش باغبانی، مؤسسه تحقیقات اصلاح و تهیه نهال و بذر، کرج

چکیده

کلکسیون هسته مجموعه‌ای از نمونه‌های گزینش شده است که معرف تنوع ژنتیکی موجود در کلکسیون پایه می باشد. این استراتژی به منظور به حداقل رساندن هزینه حفاظت از ذخائر ژنتیکی با حفظ حداکثری تنوع ژنتیکی معرفی شده است. در این روش امکان ارزیابی سریع ژرم پلاسم و دسترسی آسانتر به کلکسیون پایه فراهم می شود. در این تحقیق، به منظور ارزیابی تنوع ژنتیکی و تشکیل کلکسیون هسته، 139 نمونه Prunus dulcisMill. با منشأ متفاوت به کمک 32 نشانگر ریزماهواره و برخی از صفات کمی و کیفی، مورد بررسی قرار گرفتند. در ارزیابی های مولکولی تعداد 252 آلل مشاهده شد که میانگین تعداد آلل در هر مکان ژنی 75/7 بود. در آنالیز ساختار مبتنی بر مدل، پنج زیر جمعیت در کلکسیون پایه شناسایی شد که تا حدودی با نتایج تجزیه خوشه‌ای مبتنی بر فاصله ژنتیکی منطبق بود. با استفاده از روش اکتشافی، به ترتیب کلکسیون هسته‌ای متشکل از 20 و 112 نمونه با استفاده از نشانگرهای مولکولی و مورفولوژیک شناسایی شد که پوشش 100% داشته و کم‌ترین نمونه تکراری را دربرداشت. نتایج به دست آمده برای صفات مورفولوژیک و نشانگرهای مولکولی با یکدیگر تطابق نداشت، از این‌رو برای تاسیس کلکسیون هسته باید هر دو نشانگر مطالعه شوند. نتایج این تحقیق می تواند کمک شایانی به کشف  آلل ها، ژنتیک ارتباطی، نقشه یابی و همسانه سازی ژن‌ها، حفاظت از ژرم پلاسم و پیشرفت در برنامه‌های اصلاحی آینده بنماید.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Application of microsatellite markers and some morphological traits in core collection identification of Prunus dulcis Mill.

نویسندگان [English]

  • Mohammad Ali Ebrahimi 1
  • Motahareh Mir 2
  • Mehrshad Zinalabedini 3
  • Ali Imani 4
1 Associate Professor, Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran
2 M.Sc. Agricultural Biotechnology, Payame Noor University, Tehran, Iran
3 Assistant Professor, Department of Genomics, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran
4 Associate Professor, Department of Horticulture,Seed and Plant Improvement Institute, Karaj, Iran
چکیده [English]

A core collection consists of a set of accessions selected to represent the genetic diversity of a base collection. This strategy was introduced with the intention of minimizing the cost of genetic resource conservation, while ensuring maximum genetic variation. It also allows a rapid evaluation of germplasm, and a better access to the base collection.In this study, One hundred and thirty-nine accessions of almond with diverse origins were used for evaluation of genetic diversity and core collection establishment using 32 simple sequence repeat loci and some quantitative and qualitative traits. A total of 252 alleles were detected, which average allelic richness was 7.75 alleles per locus. The model-based structure analysis here revealed the presence of five subpopulations in the base set, which was approximatelyconsistent with clustering based on the genetic distance. Using a heuristic approach, a core set of 112 and 20 accessions was successfully developed using molecular and morphological traits respectively which showed 100% coverage of alleles with minimum redundancy. The results from molecular analyses did not correspond to morphological traits, hence to establish of core collection we have to study both morphological and molecular markers, simoltaneously. These results will provide an effective aid for future allele mining, association genetics, mapping and cloning gene(s), germplasm conservation, and promotion in breeding programs at future.

کلیدواژه‌ها [English]

  • Almond
  • Labeled- SSR marker
  • Core collection

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