با همکاری مشترک دانشگاه پیام نور و انجمن بیوتکنولوژی جمهوری اسلامی ایران

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی دوره دکتری پردیس کشاورزی و منابع طبیعی دانشگاه رازی کرمانشاه، کرمانشاه

2 استاد گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه رازی کرمانشاه، کرمانشاه

3 استاد بخش تحقیقات غلات، موسسه تحقیقات اصلاح و تهیه نهال و بذر، کرج

4 دانشیار بخش تحقیقات غلات، موسسه تحقیقات اصلاح و تهیه نهال و بذر، کرج

چکیده

در این تحقیق با استفاده از روش حذف کروموزومی تلاقی گندم و ذرت 150 لاین دابل‌هاپلویید گندم تولید شد. لاین‌های تولید شده به همراه والدین و ارقام شاهد در دو مرحله گیاهچه و گیاه کامل نسبت به بیماری‌های زنگ زرد و زنگ سیاه گندم ارزیابی شدند و هشت جایگاه ژنی مقاومت به بیماری شامل Yr5، Sr31/Yr9/Lr26، Yr15، Sr38/Yr17/Lr37، Lr34/Yr18/Pm38، Yr27، Yr36 و Yr48 در والدین جمعیت‌ها مورد بررسی قرار گرفت. نتایج این بررسی نشان داد که نشانگرهای مورد استفاده برای مکان‌های ژنی Yr5، Yr15،Yr27 و Yr36 قادر به تشخیص پلی‌مورفیسم بین والد‌ها، شاهد حساس بولانی و کنترل‎های مثبت نبودند. در مکان‌های ژنی Sr38/Yr17/Lr37 و Yr48 عدم تکثیر باندهای موردنظر بیان‌کننده عدم حضور آلل موثر این جایگاه‎های ژنی در والدین لاین‌های دابل‌هاپلویید بود. در این تحقیق بلوک ژنی Sr31/Yr9/Lr26 در ارقام MV17 و فلاندرز مشاهده شد. بررسی لاین‌های دابل‌هاپلویید نسبت به این بلوک ژنی نشان داد که تنها سه لاین از جمعیت DH-26:Ghods*3/MV17 دارای بلوک‎ژنی Sr31/Yr9/Lr26 بودند که دو لاین از آنها نسبت به نژادهای زنگ سیاه TTSTK و TTKSK واکنش مقاومت نشان دادند. آزمون ژنتیکی حضور و یا عدم حضور آلل موثر بلوک ژنی Lr34/Yr18/Pm38 بر روی والدین لاین‌های دابل‌هاپلویید بیان کننده حضور آلل موثر این بلوک ژنی در والد MV17 بود. بررسی لاین‌های دابل‌هاپلویید نسبت به این بلوک ژنی نشان داد که شش لاین دابل‌هاپلویید از جمعیت DH-26:Ghods*3/MV17 حاوی بلوک ژنی Lr34/Yr18/Pm38 بودند که تنها یک لاین از آنها نسبت به نژاد زنگ زرد 7E158A+,Yr27 دارای واکنش مقاومت در مراحل گیاهچه و گیاه کامل بود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Study on the presence of yellow and stem rust resistance genes in doubled haploid lines of bread wheat using molecular markers

نویسندگان [English]

  • Farshad Bakhtiar 1
  • Ezatollah Farshadfar 2
  • Mostafa Aghaee Sarbarzeh 3
  • Habibollah Ghazvini 4
  • Farzad Afshari 3

1 Ph.D. Student, Department of Crop Production and Plant Breeding, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran

2 Professor, Department of Crop Production and Plant Breeding, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran.

3 Professor, Cereal Research Department, Seed and Plant Improvement Institute, Karaj, Iran

4 Associate Professor, Cereal Research Department, Seed and Plant Improvement Institute, Karaj, Iran.

چکیده [English]

In this research, 150 wheat doubled haploid lines were produced using chromosome elimination method by crossing between wheat and maize. Resistance of doubled haploid lines, their parents and check cultivars against strip and stem rust was evaluated at seedling and adult plant stages. Accordingly, eight known molecular markers which are tightly linked to resistance genes including Yr5, Sr31/Yr9/Lr26, Yr15, Sr38/Yr17/Lr37, Lr34/Yr18/Pm38, Yr27, Yr36 and Yr48 were screened in parents. Results showed that molecular markers for Yr5, Yr15, Yr27 and Yr36 couldn't detect polymorphism between parents as well as positive and negative controls. For gene block Sr38/Yr17/Lr37 and locus Yr48 allele sizes were not similar to those which were expected for these genes. Results also showed that MV17 and Flanders have gene block of Sr31/Yr9/Lr26, and only 3 lines of population DH-26: Ghods*3/MV17 had this gene block from which two doubled haploid lines showed resistance reaction to TTSTK and TTKSK of Puccinia graminis pers. f.sp tritici races. Genetic test for the presence or absence of gene block Lr34/Yr18/Pm38 on parents of doubled haploid lines showed that MV17 comprises this gene block. Evaluation of doubled haploid lines for this gene block showed that 6 doubled haploid lines of population DH-26:Ghods*3/MV17 have gene block Lr34/Yr18/Pm38 for which only one doubled haploid line showed resistance reaction to Puccinia striiformis Westend f.sp. tritici race of 7E158A+, Yr27 at both seedling and adult plant stages.

کلیدواژه‌ها [English]

  • Wheat
  • Rust disease
  • Molecular markers
  • Doubled haploid
Alexopoulos CJ, Mims CW (1997) Introductory mycology, third ed. pp.632.
Bakhtiar F, Bozorgipour R, Shahabi S (2006) Production of doubled haploid lines of wheat using detached tillering method in cross between wheat and maize, and evaluation of some agronomic characters. Seed and Plant .22-3:351-367. (in Persian)
Bariana HS, Hayden MJ, Ahmed NU, Bell JA, Sharp PJ, McIntosh RA (2001) Mapping of durable adult plant and seedling resistances to stripe rust and stem rust diseases in wheat. Aust. J. Agric. Res. 52:1247–1255.
Basnet BR, Singh S, Lopez-Vera EE, Huerta-Espino J, Bhavani S, Jin Y, Rouse MN, Singh RP (2015) Molecular mapping and validation of SrND643: A new wheat gene for resistance to stem rust pathogen Ug99 race group. Phytopathology 105:470-476.
Chen XM, Soria MA, Yan GP, Sun J, Dubcovsky J (2003) Development of Sequence Tagged Site and Cleaved Amplified Polymorphic Sequence Markers for Wheat Stripe Rust Resistance Gene Yr5. In: Crop Science 43: 2058-2064.
Chen XM (2005) Epidemiology and control of strip rust (Puccinia striiformis f.sp.tritici) on wheat. Canadian Journal of Plant pathology 27: 314 – 337.
Dakori A, Brent D, Callum M, Andrzej Z, Cloutier S (2010) Fine-mapping of the leaf rust Lr34 locus in triticum aestivum L. and characterization of large germplasm collections support the ABC transporter as essential for gene function. Theor. Appl. Genet. 121: 373-384
Distelfeld A, Uauy C, Fahima T, Dubcovsky J (2006) Physical map of the wheat high-grain protein content gene Gpc-B1 and development of a high-throughput molecular marker. In: New Phytologist, 169:753-763
Dyck PL (1987) The association of a gene for leaf rust resistance with the chromosome 7D suppressor of stem rust resistance in common wheat. Genome 29: 467-469.
Eriksen L, Afshari F, Christiansen MJ, McIntosh RA, Jahoor A, Wellings CR, (2004) Yr32 for resistance to stripe (yellow) rust present in wheat cultivar Carstens V. Theor Appl Genet 108: 567-575.
Gerechter-Amitai ZK, Stubbs RW (1970) A valuable source of yellow rust resistance in Israeli populations of wild emmer, Triticum dicoccoides Koern. Euphytica 19: 12-21.
Gupta Varshney RK, Roy JK, Prasad M (1999a) Development of molecular markers for wheat breeding at Meerut, a center of wheat biotechnology network in India. Annu.wheat News, in press, 1-45.
Helguera M, Khan IA, Kolmer J, Lijavetzky D, Zhang-qi L, Dubcovsky J (2003) PCR assay for the Lr37- Yr17- Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines. Crop Sci. 43: 1839-1847.
Johnson R (1981) Durable resistance definition of genetic control, and attainment in plant breeding. Phytopathology. 71(8): 567-568.
Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323: 1360–1363.
Line RF, Chen XM (1995) Successes in breeding for and managing durable resistance to wheat rusts. Plant Dis 79: 1254–1255
Lowe I, Jankuloski L, Chao SM, Chen XM, See D, Dubcovsky J (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat. In: Theoretical and Applied Genetics, 123:143-157.
Macer RCF (1966) The formal and monosomic genetic analysis of strip rust puccinia striformis resistance in wheat. In Maker, J. (ed). Proceeding of the Second International Wheat Genetic Symposium. Lund, Sweden 1963, Hereditas Supplement. 2: 127-142.
McDonald DB, McIntosh RA, Wellings CR, Singh RP, Nelson JC (2004) Cytogenetical studies in wheat XIX. Location and linkage studies on gene Yr27 for resistance to stripe (yellow) rust. Euphytica 136: 239-248.
McIntosh RA, Hart GE, Devos KM, Gale MD (1995) Catalogue of gene symbols for wheal: 1995 supplement. Wheat Information Service 81:22-49.
McIntosh RA, Wellings CR, Park RF (1995) Wheat Rusts: An Atlas of Resistance Genes. CSIRO East Melbourne, Australia.
McIntosh RA, Silk J (1996) Cytogenetic studies in wheat XVII. Monosomic analysis and linkage relationships of gene Yr15 for resistance to stripe rust: Euphytica, 89(3): 395-399.
McIntosh RA, Yamazaki Y, Devos KM, Dubcovsky J, Rogers WJ, Appels R (2003) Catalogue of gene symbols for wheat. In: Proc. 10th Int Wheat Genet Symp, Vol. 4. Instituto Sperimen-tale per la Cerealicoltura, Rome, Italy.
McIntosh RA, Devos KM, Dubcovsky J, Rogers WJ, Morris CF, Appels R, Anderson OA (2005) Catalogue of gene symbols for wheat: 2005 supplement http://wheat.pw.usda.gov
McNeal FH, Konzak CF, Smith EP, Tate WS, Russell TS (1971) 'A Uniform System for Recording and Processing Cereal Research Data.' Agricultural Research Service Bulletin 34-121. (United States Department of Agriculture: Washington.)
Mehrabi R, Sarhangi M, Ala-hassani E, Ghazvini H, Afshari F (2015) Study on present of resistance gene Loic to yellow, Stem and leaf rust diseases using molecular marker in pre-released wheat lines. Crop Biotech.7: 49-58.
Milus EA, Line RF (1986a) Number of genes controlling high temperature adult-plant resistance to stripe rust in wheat. Phytopathology. 76: 93-96.
Mohammadi M, Torkamaneh D, Patpour M (2013) Seedling stage resistance of Iranian bread wheat germplasm to race Ug99 of puccinia graminis f.sp. tritici. Plant Dis. 97: 387-392.
Nyquist NE (1962) Differential fertilization in the inheritance of stem rust resistance in hybrids involving a common wheat strain derived from Triticumtimopheevii. Genetics 47:1109-1124.
Peng JH, Fahima T, Roeder MS, Huang QY, Dahan A, Li YC, Grama A, Nevo E (2000) High-density molecular map of chromosome region harboring stripe-rust resistance genes YrH52 and Yr15 derived from wild emmer wheat, Triticum dicoccoides. In: Genetica, 109(3): 199-210.
Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity of leaves and stems of cereals. Canadian Journal of Research Section C. 26: 496-500.
Qayoum A, Line RF (1985) High-temperature, adult-plant resistance to stripe rust of wheat. Phytopathology 75: 1121-1125
Roelfs AP (1978) Estimated losses caused by rust in small grain cereal in the United States during 1918-1976. Miscellaneous publication 1363. (United States Department of Agriculture; Washington DC.)
Roelfs AP, Singh RP, Saari EE (1992) Rust disease of wheat: concepts and method of disease management. Mexico, D.F.: CIMMYT. pp: 81.
Saari EE, Prescott JM (1985) World distribution in relation to economic loss. pp. 259–289, in: Roelfs AP, Bushnell WR (editors). The cereal rusts. Vol. 2: Diseases, distribution, epidemiology and control. Academic Press, Orlando, FL, USA.
Saghai-maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci. 81: 8014-8019.
Seah S, Spielermeyer W, Jahier J, Sivasithamparam K, Lagudah ES (2000) Resistance gene analogs within an introgressed chromosomal segment derived from Triticum ventricosum that confers resistance to nematode and rust pathogens in wheat. Mol. Plant-microbe Interact. 13: 344-341.
Singh RP (1992b) Genetic association between gene Lr34 for leaf rust resistance and leaf tip necrosis in bread wheats. Crop Sci. 32: 874-878.
Singh RP (2004) Genetics and breeding for durable resistance to leaf and strip rusts in wheat.pp.213.
Snape JW (1989) Doubled haploid breeding, theoretical basis and practical applications. In Mujeeb-Kazi A,. and Sitch LA (Eds). Review of Advances in plant Biotechnology, 1985-88, pp: 19-30. International Maize and Wheat Improvement Center, Mexico/ International Rice Research Institute, P. o. Box 933, Manila, Philippines.
Spielmeyer W, Macintosh RA, Kolmer J, Lagudah ES (2005) Powdery mildew resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe rust co-segregate at a locus on the short arm of chromosome 7D of wheat. Theor. Appl. Genet. 11: 731-735.
Torabi M, Mardoukhi V, Nazari K, Afshari F, Forootan AR, Rami MA, Golzar H Kashani AS (1995) Effectiveness of wheat yellow rust resistance genes in different parts of Iran. Cereal Rust and Powdery Mildow Bulletin. 23: 9-12.
Tsilo TJ, Jin Y, Anderson JA (2008) Diagnostic microsatellite markers for thedetection of stem rust resistance gene Sr36 in diverse genetic backgrounds of wheat. Crop Sci. 48: 253-261.
Wellings CR (1992) Resistance to stripe (yellow) rust in selected spring wheats. Vortr¨age fur Pflanzenz¨uchtung 24: 273-275.
Wu S, Pumphrey M, Bai G (2009) Molecular mapping of stem rust-resistance gene Sr40 in wheat. Crop Sci. 49: 1682-1686.