آنالیز ترانسکریپتوم بافت برگ ارقام جو متفاوت در تشکیل زیست توده در مرحله زایشی

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی دکتری، موسسه بیوشیمی و بیوتکنولوژی، دانشگاه هاله ویتنبرگ، هاله، آلمان.

2 استاد، بخش تغذیه ملکولی، دانشگاه هاله ویتنبرگ، هاله، آلمان

3 استاد، موسسه بیوشیمی و بیوتکنولوژی، دانشگاه هاله ویتنبرگ، هاله، آلمان.

4 استاد، بخش ریزازدیادی، موسسه گیاهان زینتی و سبزی صیفی، ایرفورت، آلمان

چکیده

کشف همبستگی میان تجمع زیست‌توده در گیاه و عملکرد، پیش نیاز درک ارتباط بین داده های امیکس و میزان رشد در گیاهان است. برای جستجوی این ارتباط بین ترانسکریپت‌ها و نقش تنظیمی آنها در تشکیل زیست‌توده لیگنوسلولزی در فاز زایشی جو، پروفایل ترانسکریپت‌ها دو هفته پس از گلدهی در 3 لاین جو بهاره مورد استفاده قرار گرفت. یک ریزآرایه سفارشی (custom microarray) متشکل از 56000 اولیگونوکلئوتید برای آنالیز ترانسکریپتوم برگ پرچم در مرحله زایشی استفاده شد. شبکه ی همبستگی ترانسکریپت‌های درگیر در متابولیسم ثانویه و متابولیسم RNA، دارای تعداد بیشتری همبستگی مثبت نسبت به منفی بود که از این بین یک سیگنال ملکول،ABH1-Cap binding protein بالاترین میزان اتصال را به دیگر ترانسکریپت‌ها نشان داد. آنالیز آماری یک همبستگی مثبت بین ABH1-Cap binding protein و یک ژن کلیدی مسیر فنیل پروپانویید، به نام Cinnamoyl-COA reductase نشان داد. تلفیق داده های بدست آمده پیشنهاد کننده ی این موضوع هستند که ژن (CCR) Cinnamoyl-COA reductase ممکن است بتوانند به‌عنوان بیومارکر برای مهندسی اصلاح زیست‌توده لیگنوسلولزی در فاز زایشی جو استفاده شود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Transcriptome analysis of leaf tissue in contrasting lines of barley for biomass formation at the reproductive stage

نویسندگان [English]

  • Mohammad Reza Ghaffari 1
  • Nicolaus von Wirén 2
  • Klaus Humbeck 3
  • Phillip Franken 4
1 Ph.D. student, Institute for Biochemistry and Biotechnology, Martin Luther University of Halle-Wittenberg, Halle, Germany.
2 Molecular Plant Nutrition Group, Martin Luther University of Halle-Wittenberg, Halle, Germany
3 Institute for Biochemistry and Biotechnology, Martin Luther University of Halle-Wittenberg, Halle, Germany.
4 Department Plant Propagation, Leibniz Institute of Vegetable and Ornamental Crops, Erfurt, Germany
چکیده [English]

Deciphering of network correlation operating in the plant biomass accumulation and yield production is a pre-requisite for understanding the relationships between omics data and growth rate in plants. To investigate the relationship among transcripts and their regulation for lignocellulose biomass formation at the generative stage of barley, transcript profiling was applied on three contrasting spring barley lines two weeks after flowering. A custom barley cDNA Microarray (Agilent Technologies, Germany) containing 56000 barley oligonucleotides was used for transcriptome analysis on flag leaves of spring barley. The network correlation of transcripts involved in secondary and RNA metabolism revealed a higher number of positive than negative correlations of which a signal molecule, ABH1-Cap binding protein showed the highest node degree centrality. Statistical test showed a strong positive interrelation between ABH1-Cap binding protein and a key gene of phenylpropanoid pathway, Cinnamoyl-CoA reductase. The integrated data suggested Cinnamoyl-CoA reductase (CCR) might be used as putative biomarker for engineering of lignocellulose biomass improvement at the generative stage in barley.

کلیدواژه‌ها [English]

  • Plant biomass
  • Transcriptome analysis
  • Network correlation analysis
  • Barley
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