بررسی حضور ژن مقاومت به زنگ سیاه Sr2 در ارقام و لاین های امید بخش گندم ایران با استفاده از نشانگرهای مولکولی

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشیار مؤسسه تحقیقات اصلاح و تهیه نهال و بذر کرج، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

2 کارشناس آزمایشگاه، مؤسسه تحقیقات اصلاح و تهیه نهال و بذر کرج، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

چکیده

زنگ سیاه گندم (Puccinia graminis) به عنوان یکی از مهم‌ترین و رایج ترین بیماری های گندم در اغلب کشورهای جهان شناخته می‌شود. در دهه‌های آخر قرن بیستم به کار گرفتن تعدادی از ژن های مقاومت به زنگ سیاه مانند Sr24، Sr26، Sr31، Sr36 و Sr38 در ارقام گندم باعث شد خسارت ناشی از بیماری به طور چشم‌گیری کاهش یابد. یکی از ژن هایی که باعث مقاومت پایدار نسبت به زنگ سیاه در این دوره بود، ژن Sr2 است که از گندم تورجیدوم(Triticum turgidum) به گندم معمولی انتقال یافته است. ژن Sr2 باعث القای مقاومت در مرحله گیاه بالغ می گردد و ژنی با مقاومت غیر اختصاصی (Non-race specific resistance) با اثر کاهش بیماری زنگ (Slow rusting) می باشد. در سال های اخیر، نشانگرهای مولکولی پیوسته با ژن Sr2 شناسایی شده و در بسیاری از کشورها مورد استفاده قرار گرفته اند. در این تحقیق حضور ژن Sr2 در تعدادی از ارقام و لاین های امیدبخش گندم کشور با استفاده از یک نشانگر ریز ماهواره gwm533 و یک نشانگر CAPS انجام گردید. نتایج نشان داد که از مجموع 188 ژنوتیپ مورد بررسی، آلل های پیوسته با ژن Sr2 در 16 ژنوتیپ وجود داشتند. نتایج این تحقیق همچنین بیانگر این بود که نشانگر جدید CAPS دقت بسیار بالاتری نسبت به نشانگر gwm533 در تعیین ژن Sr2 در ژنوتیپ های گندم دارد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Study on presence of stem rust resistance gene Sr2 in the Iranian varieties and elite wheat lines by using molecular markers

نویسندگان [English]

  • Habibollah Ghazvini 1
  • Mohsen Mohsen 2
1 Associate Professor, Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
2 Laboratory Technician, Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
چکیده [English]

Stem rust of wheat caused by Puccinia graminis is known as one of the most important and prevalent diseases of wheat worldwide. Deployment of stem rust resistance genes such as Sr24, Sr26, Sr31, Sr36 and Sr38 in wheat cultivars in the last decades of 20th century dramatically reduced damaged caused by this disease. One of the durable resistance genes that controlled stem rust during this period was Sr2 which has been transferred to bread wheat from Triticum turgidum. Sr2 is a non-race specific resistance gene with slow rusting effect which confers resistance at the adult plant stage. In recent years, molecular markers which are tightly linked to Sr2 have been identified and are widely used in many countries. In this study, presence/absence of Sr2 in some of the Iranian varieties and elite wheat lines was tested using a SSR marker gwm533, and a CAPS marker. Results indicated that from 188 studied genotypes, only 16 genotypes had linked alleles to Sr2 genes. Results of this study also showed that the newly identified CAPS marker is more precise marker compared to SSR marker gwm533 to detect Sr2 gene.

کلیدواژه‌ها [English]

  • Marker assisted breeding
  • Pyramiding of resistance gene
  • Adult plant resistance
Afshari (2014) Determination of number of resistance genes to stem rust Disease (Puccinia graminis f.sp. tritici), rtace Ug99 in two wheat cultivars. Agricultural Biotechnology 4: 27-33.
Elahinia SA (2005) Crop diseases and their control. University of Gilan Presse. 638 p.
FAO (2014) OECD-FAO Agricultural Outlook 2014, Wheat projections: Consumption, food use, per capita (DOI :10.1787/agr_outlook-2014-en). Retrieved from: http://www.oecd-ilibrary.org/agriculture-and-food/oecd-fao-agricultural-outlook-2014/statistical-annex_agr_outlook-2014-16-en in March 8th, 2016.
Ghazvini H (2012) Emergence and current status of Ug99 races of Puccinia graminis f. sp. tritici and overview of recent progresses in deployment of stem rust resistance genes for effective control of disease (keynote presentation). Proceedings Of 12th Congress of Agronomy and Plant Breeding, Karaj, September 4-6, 2012.
Ghazvini H, Hiebert CW, Zegeye T, Liu S, Dilawari M, Anderson JA, Rouse MN, Jin Y, Fetch T (2012a) Inheritance of resistance to Ug99 stem rust in wheat cultivar Norin 40 and genetic mapping of Sr42. Theor. Appl. Genet. 125: 817-824.
Ghazvini H, Hiebert CW, Zegeye T, Fetch T (2012b) Inheritance of stem rust resistance derived from Aegilops triuncialis in wheat line Tr129. Can. J. Plant Sci. 92: 1037-1041.
Ghazvini H, Hiebert CW, Thomas JB, Fetch T (2013) Development of a multiple bulked segregant analysis (MBSA) method used to locate a new stem rust resistance gene (Sr54) in the winter wheat cultivar Norin 40. Theor. Appl. Genet. 126(2): 443-449.
Hayden MJ, Kuchel H, Chalmers KJ (2004) Sequence tagged microsatellites for the Xgwm533 locus provide new diagnostic markers to select for the presence of stem rust resistance gene Sr2 in bread wheat (Triticum aestivum L.). Theor.. Appl. Genet. 109: 1641–1647.
Hiebert CW, Fetch TG, Zegeye T (2010) Genetics and mapping of stem rust resistance to Ug99 in the wheat cultivar Webster. Theor. Appl. Genet. 121: 65–69.
Jin Y, Singh RP, Ward RW, Wanyera R, Kinyua M, Njau P, Fetch T, Pretorius ZA, Yahyaoui A (2007) Characterization of seedling infection types and adult plant infection responses of monogenic Sr gene lines to race TTKS of Puccinia graminis f. sp. tritici. Plant Dis. 91: 1096–1099.
Jin Y, Szabo L, Pretorius ZA, Singh R, Ward R, Fetch T (2008) Detection of virulence to resistance gene Sr24 within race TTKS of Puccinia graminis f. sp. tritici. Plant Dis. 92: 923–926.
Jin Y, Szabo L, Rouse M, Fetch JT, Pretorius ZA, Wanyera R, Njau P (2009) Detection of virulence to resistance gene Sr36 within race TTKS lineage of Puccinia graminis f. sp. tritici. Plant Dis. 93: 367–370.
Long-Xi Y, Sixin L, Anderson JA, Singh RP, Yue J, Dubcovsky J, Brown-Guidera G, Bhavani S, Morgounov A, Zhonghu H; Huerta-Espino J, Sorrells ME (2010) Haplotype diversity of stem rust resistance loci in uncharacterized wheat lines. Mol. Breeding. 26: 667–680.
Mago R, Brown-Guedira G, Dreisigacker S, Breen J, Jin Y, Singh R, Appels R, Lagudah ES, Ellis J, Spielmeyer W (2011) An accurate DNA marker assay for stem rust resistance gene Sr2 in wheat. Theor. Appl. Genet. 122: 735-744.
McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. CSIRO Publications, East Melbourne.
McNeil MD, Kota R, Paux E, Dunn D, McLean R, Feuillet C, LD, Kong X, Lagudah E, Zhang JC, Jia JZ, Spielmeyer W, Bellgard M, Appels R (2008) BAC-derived markers for assaying the stem rust resistance gene, Sr2, in wheat breeding programs. Mol. Breeding 22: 15–24.
Mohammadi M, Torkamaneh D, Patpour M (2013) Seedling stage resistance of Iranian bread wheat germplasm to race Ug99 of Puccinia graminis  f. sp.  tritici. Plant Dis. 97: 387-392.
Najafian G, Amin H, Afshari F, Rajaei S, Nikzad AR (2010) Parsi, a new bread wheat cultivar, resistant to stem rust (race Ug99) with good bread making quality for cultivation under irrigated conditions of temperate regions of Iran. Seed and Plant Improvement Journal. 26 (2): 289-292.
Nazari K, Mafi M, Yahyaoui A, Singh RP, Park RF (2009) Detection of wheat stem rust (Puccinia graminis f. sp. tritici) race TTKSK (Ug99) in Iran. Plant Dis. 93: 317.
Park R, Fetch T, Hodson D, Jin Y, Nazari K, Prashar M, Pretorius Z (2011) International surveillance of wheat rust pathogen: progress and challenges. Euphytica. 179: 109-117.
Patpour M, Nazari K, Alavi SM, Mousavi A (2014) Detection of resistance sources to Iranian prevalent stem rust races in commercial wheat cultivars. Seed and Plant Improvement Journal 30 (1): 133-154.
Peturson B (1958) Wheat rust epidemics in western Canada in 1953, 1954, and 1955. Can. J. Plant Sci. 38: 16–28.
Pretorius ZA, Singh RP, Wagoire WW, Payne TS (2000) Detection of virulence to wheat stem rust resistance gene Sr31 in Puccinia graminis f.sp. tritici in Uganda. Plant Dis. 84: 203.
Roelfs AP, Martens JW (1988) An international system of nomenclature for Puccinia graminis f. sp. tritici. Phytopathology. 78: 526–533.
Roelfs AP, Singh RP, Saari EE (1992) Rusts Diseases of Wheat: Concepts and Methods of Disease Management. Mexico, D.F. CIMMYT.
Saari EE, Prescott JM (1985) World distribution in relation to economic losses. In The Cereal Rusts, Vol II: Diseases, Distribution, Epidemiology, and Control, ed. AP Roelfs,WR Bushnell, pp. 259–98. Orlando, FL: Acad. Press.
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA sepacer-length polymorphism in barley: mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci. 81: 8014–8019.
Singh RP, Hodson DP,  Huerta-Espino J, Jin Y, Bhavani S, Njau P, Herrera-Foessel S, Singh PK, Singh S, Govindan V (2011b) The emergence of Ug99 races of the stem rust fungus is a threat to world wheat production. Annu. Rev. Phytopathol.49: 13.1–13.17.
Singh RP, Hodson DP, Huerta-Espino J, Jin Y, Njau P, Wanyera R, Herrera-Foessel SA, Ward WR (2008) Will stem rust destroy the world’s wheat crop? Adv. Agron. 98: 271–309.
Singh RP, J Huerta-Espino S. Bhavani SA, Herrera-Foessel D, Singh PK, Singh G, Velu RE, Mason Y, Jin P, Njau J (2011a) Race non-specific resistance to rust diseases in CIMMYT spring wheats. Euphytica. 179: 175-186.
Spielmeyer W, Sharp PJ, Lagudah ES (2003) Identification and validation of markers linked to broad-spectrum stem rust resistance gene Sr2 in wheat (Triticum aestivum L.). Crop Sci. 43: 333–336.
Stakman EC, Harrar JG (1957) Principles of plant pathology. Ronald Press. New York.
Sunderwirth SD, Roelfs AP (1980) Greenhouse evaluation of the adult plant resistance of Sr2 to wheat stem rust. Phytopathology. 70: 634-637.
Wanyera R, Kinyua MG, Jin Y, Singh RP (2006) The spread of stem rust caused by Puccinia graminis f. sp. tritici, with virulence on Sr31 in wheat in Eastern Africa. Plant Dis. 90: 113.