بررسی پروتئین های مشترک پاسخ دهنده به تنش خشکی در دو رقم حساس و متحمل گندم بهاره

نوع مقاله : علمی پژوهشی

نویسندگان

استادیار بخش کشاورزی، دانشگاه پیام نور، تهران، ایران

چکیده

در این پژوهش، تجزیه پروتئوم از طریق الکتروفورز دوبعدی و رنگ-آمیزی آبی کوماسی برای دو رقم گندم بهاره شامل کویر به‌عنوان رقم متحمل و بهار به‌عنوان رقم حساس انجام شد. بعد از کمّی کردن لکه-ها توسط نرم‌افزارSame spot progenesis ، تعداد 10 لکه پروتئینی مشترک تکرارپذیر دارای تفاوت معنی‌دار بین شرایط شاهد و تنش خشکی در دو رقم کویر و بهار تشخیص داده شد. با استفاده از طیف‌سنجی جرمی MALDI-TOF/TOFاز بین پروتئین‌های مشترک، نه لکه پروتئینی مشترک شناسایی و نوع فعالیت آنها و نحوه عمل این پروتئین‌ها در سلول مشخص شد. بر اساس نتایج بدست آمده پروتئین‌های دخیل در واکنش نوری (سه لکه پروتئینی شاملChlorophyll a-b binding protein 8، Cytochrome b6-f complex iron-sulfur subunit, chloroplasti و Peptidyl-prolyl cis-trans isomerase CYP38) و چرخه کالوین (دو لکه پروتئینی شامل Fructose-1,6-bisphosphatase و ribulose-bisphosphate carboxylase small chain precursor) در هر دو رقم بیشترین گروه‌های عملکردی پروتئین و به عبارتی مهمترین پروتئین-های مشترک جهت حفظ کارایی گندم تحت شرایط تنش خشکی بودند. از آنجایی که این پروتئین‌ها در هر دو رقم متحمل و حساس تغییر بیان نشان دادند، بنظر می‌رسد که حساس‌ترین پروتئین‌ها در پاسخ به تنش خشکی در گندم بهاره باشند. در مجموع، نتایج این پژوهش نشان داد که حفظ کارایی فتوسنتز تحت تنش خشکی از اهمیت بالایی برخوردار است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Evaluation of responsive common proteins in tolerant and sensitive spring wheat cultivars under drought stress

نویسندگان [English]

  • Marouf Khalili
  • Mohammad Reza Naghavi
Assistant Professor, Department of Agriculture, Payame Noor University, P.O. Box 19395-3697 Tehran, Iran
چکیده [English]

In this research, proteome analysis was done by two-dimensional electrophoresis and stainig by Commassie brilliant blue method for two cultivars of Kavir as tolerant and Bahar as susceptible was done. After of be quantitative spots with Same spot progenesis software, 10 common protein spots with significant difference between control and drought stress conditions in the Kavir and Bahar was diagnosed. Using MALDI-TOF/TOF mass spectrometry of common proteins, nine common protein spots were identified and the type of activity and mode of action of these proteins in the cells was determined. Based on the results, proteins involved in light reactions of photosynthesis (three protein spots Chlorophyll ab binding protein 8, chloroplastic, Cytochrome b6-f complex iron-sulfur subunit, chloroplasti and Peptidyl-prolyl cis-trans isomerase CYP38, chloroplastic) and Calvin cycle (two protein spots include Fructose-1,6-bisphosphatase, chloroplastic and ribulose-bisphosphate carboxylase small chain precursor) in both cultivars were the greatest functional groups and in other words, the most important common proteins for maintain of efficiency under drought stress were. Since these proteins in both tolerant and susceptible cultivars showed changes in expression, seems to be the most sensitive proteins in response to drought stress in wheat. In other words, these results show that it is important to maintain the efficiency of photosynthesis under drought stress.

کلیدواژه‌ها [English]

  • Drought stress
  • photosynthesis
  • responsive common protein
  • Wheat

مراجع

Ali GM, Komatsu S (2006) Proteomic Analysis of Rice Leaf Sheath during Drought Stress. J. Proteome Res. 5(2): 396-403.
Allen JF (2003) Cyclic, pseudocyclic and noncyclic photophosphorylation: new links in the chain. Trends Plant Sci. 8: 15-19.
Aranjuelo I, Molero G, Erice G, Christophe Avice J, Nogues S (2011) Plant physiology and proteomics reveals the leaf response to drought in alfalfa (Medicago sativa L.). J. Exp. Bot. 62(1): 111-123.
Arnau GP, Monneveux D, This D, Alegre L (1997) Photosynthesis of six barley genotypes as affected by water stress. Photosynthetica, 34: 67-76.
Bassi R, Sandona D, Croe R (1997). Novel aspects of chlorophyll a/b-binding proteins. Physiol. Plantarum, 100: 769-779.
Boehlein SK, Sewell AK, Cross J, Stewart JD, Hannah LC (2005) Purification and characterization of adenosine diphosphate glucose pyrophosphorylase from maize/potato mosaics. Plant Physiol. 138: 1552-1562.
Caruso G, Cavaliere C, Foglia P, Gubbiotti R, Samperi R, Laganà A (2009) Analysis of drought responsive proteins in wheat (Triticum durum) by 2D-PAGE and MALDI-TOF mass spectrometry. Plant Sci. 177: 570-576.
Costa R, Bahrman N, Frigerio JM, Kremer A, Plomion C (1998) Water deficit-response proteins in maritime pines. Plant Mol. Biol. 38: 587-596.
Cui S, Huang F, Wang J, Ma X, Cheng Y, Liu J (2005) A proteomic analysis of cold stress responses in rice seedlings. Proteomics, 5(12): 3162-3172.
Damerval C, De Vienne D, Zivy M, Thiellement H (1986) Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat-seedling roteins. Electrophoresis, 7: 52-4.
Demirevska K, Zasheva D, Dimitrov R, Simova-Stoilova L, Stamenova M, Feller U (2009) Drought stress effects on rubisco in wheat: Changes in the rubisco large subunit. Acta Physiol. Plantarum, 31: 1129-1138.
Firoozi B, Sofalian O, Shokrpour M, Rasoulzadeh A, Ahmadpoor F (2012) Assessment of Drought Tolerance Indices and their Relation with ISSR Markers in Bread Wheat (Triticum aestivum L.). Not. Sci. Biol. 4(3): 143-150.
Flexas J, Medrano H (2002) Drought-inhibition of photosynthesis in C3 plants: stomatal and non-stomatal limitations revisited. Ann. Bot. 89(2): 183-189.
Guan HP, Keeling PL (1998). Starch Biosynthesis: Understanding the functions and interactions of multiple isoenzymes of starch synthase and branching enzyme. Trends Glycosci. Glyc. 10: 307-319.
Han F, Chen H, Yan MF, Liu GS, Shen SH (2009) A comparative proteomic analysis of rice seedlings under various high-temperature stresses. Biochimica et Biophysica Acta, 1794(11): 1625-1634.
Hashimoto M, Komatsu S (2007) Proteomic analysis of rice seedling during cold stress. Proteomics, 7: 293-302.
Heide H, Kalisz HM, Follmann H (2004) The oxygen evolving enhancer protein 1 (OEE) of photosystem II in green algae exhibits thioredoxin activity. J. Plant Physiol. 161: 139-149.
Heldt HW (1997) Plant biochemistry and molecular biology. Oxford: Oxford University Press.
Ifuku K, Ishihara S, Shimamoto R, Ido K, Sato F (2008) Structure, function, and evolution of the PsbP protein family in higher plants. Photosynth. Res. 98: 427-437.
Ifuku K, Nakatsu T, Shimamoto R, Yamamoto Y, Ishihara S, Kato H, Sato F (2005) Structure and function of the PsbP protein of photosystem II from higher plants. Photosynth. Res. 84: 251-255.
Ji K, Wang Y, Sun W, Lou Q, Mei H, Shen S, Chen H (2012) Drought-responsive mechanisms in rice genotypes with contrasting drought tolerance during reproductive stage. J Plant Physiol. 169: 336-344.
Kausar R, Arshad M, Shahzad A, Komatsu S (2013). Proteomics analysis of sensitive and tolerant barley genotypes under drought stress. Amino Acids, 44: 345-359.
Kim SH, Mizuno K, Fujimura T (2002) Regulated expression of ADPglucose pyrophosphorylase and chalcone synthase during root development in sweet potato. Plant Growth Regul. 38: 173-179.
Komatsu S, Tanaka N (2004) Rice proteome analysis: A step toward function analysis of the rice genome. Proteomics, 4: 938-949.
Kruger NJ (1997) Carbohydrate synthesis and degradation. In: Dennis DT, Turpin DH, Lefebvre DD, Layzell DB (Eds.), Plant Metabolism, 2nd Ed, Essex: Addison Wesley Longman, pp 83-104.
Lepedu H, Toma A, Juri SA, Katani Z, Cesar V, Fulgosi H (2009). Photochemistry of PSII in CYP38 Arabidopsis thaliana. Food Technol. Biotechnol. 47(3): 275-280.
Macdonald FD, Buchanan BB (1997) The reductive pentose phosphate pathway and its regulation. In:  Dennis DT, Turpin DH, Lefebvre DD, Layzell DB (Eds.), Plant Metabolism, 2nd Ed. Essex: Addison Wesley Longman, pp 299-313.
Mohanty N (2003) Photosynthetic characteristics and enzymatic antioxidant capacity of flag leaf and the grain yield in two cultivars of (Triticum aestivum L.) exposed to warmer growth conditions.  J Plant Physiol. 160: 71-74.
Moller AL, Pedas P, Andersen B, Svensson B, Schjoerring JK, Finnie C (2011) Responses of barley root and shoot proteomes to longterm nitrogen deficiency, short-term nitrogen starvation and ammonium. Plant Cell Environ. 34(12): 2024-2037.
Mushtaq R, Katiyar S, Bennett J (2008) Proteomic analysis of drought stress-responsive proteins in rice endosperm affecting grain quality. J. Crop Sci. Biotechnol. 11: 227-232.
Naghavi MR, Moghaddam M, Toorchi M, Shakiba MR (2014) Evaluation of spring wheat cultivars under drought stress and proteome analysis for the most tolerant and sensitive ones. PhD Thesis, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Iran. (In Persian).
Riccardi F, Gazeau P Jacquemot M-P, Vincent D, Zivy M (2004) Deciphering genetic variation of proteome responses to water deficit in maize leaves. Plant Physiol. Biochem. 42: 1003-1011.
Ruuska SA, Andrews TJ, Badger MR, Price GD, von Caemmerer S (2000) The role of chloroplast electron transport and metabolites in modulating Rubisco activity in tobacco: insights from transgenic plants with reduced amounts of cytochrome b/f complex or glyceraldehyde 3-phosphate dehydrogenase. Plant Physiol. 122: 491-504.
Santos C, Pereira A, Pereira S Teixeira J (2004) Regulation of glutamine synthetase expression in sunflower cells exposed to salt and osmotic stress. Sci. Hort. 103: 101-111.
Spreitzer RJ, Salvucci ME (2002) Rubisco: structure, regulatory interactions, and possibilities for a better enzyme. Annu. Rev. Plant Biol. 53: 449-475.
Stéphanie M, Bernard Z, Habash D (2009) The importance of cytosolic glutamine synthetase in nitrogen assimilation and recycling. New Phytol. 182: 608-620.
Takahashi S, Murata N (2008) How do environmental stresses accelerate photo inhibition? Trends Plant Sci. 13: 178-182.
Tamoi M, Nagaoka M, Yabuta Y, Shigeoka S (2005) Carbon metabolism in the Calvin cycle. Plant Biotechnol. 22: 355-360.
Tamura T, Hara K, Yamaguchi Y, Koizumi N, Sano H (2003) Osmotic stress tolerance of transgenic tobacco expressing a gene encoding a membrane-located receptor-like protein from tobacco plants. Plant Physiol. 131: 454-462.
Tetlow IJ, Davies EJ, Vardy KA, Bowsher CG, Burrell MM, Emes MJ (2003) Subcellular localization of ADPglucose pyrophosphorylase in developing wheat endosperm and analysis of the properties of a plastidial isoform. J. Exp. Bot. 54: 715-725.
Tezara W, Mitchell V, Driscoll S, Lawlor D (1999). Water stress inhibits plant photosynthesis by decreasing coupling factor and ATP. Nature, 401: 914-917.
Turkan I, Bor M, Ozdemir F, Koca H (2005) Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci. 168: 223-231.
von Ballmoos C, Dimroth P (2007) Two distinct proton binding sites in the ATP synthase family. Biochemistry, 46: 11800-11809.
Xue G-P, Mcintyre CL, Glassop D, Shorter R (2008) Use of expression analysis to dissect alterations in carbohydrate metabolism in wheat leaves during drought stress. Plant Mol. Biol. 67: 197-214.
Ye J, Wang S, Zhang F, Xie D, Yao Y (2013) Proteomic analysis of leaves of different wheat genotypes subjected to PEG6000 stress and rewatering. Plant Omics J. 6(4): 286-294.
Yordanov I, Velikova V, Tsonev T (2000) Plant responses to drought, acclimation and stress tolerance. Photosynthetica, 38: 171-186.
Yordanov I, Velikova V, Tsoney T (2003) Plant responses to drought and stress tolerance. Bulgarian J. Plant Physiol. Special Issue. 187-206.
Zang X, Komatsu S (2007) A proteomic approach for identifying osmotic-stress-relatedproteins in rice. Phytochem. 68: 426-437.
Zhu M, Simons B, Zhu N, David G, Oppenheimer M, Chen S (2010) Analysis of abscisic acid responsive proteins in Brassica napus guard cells by multiplexed isobaric tagging. J. Proteomics, 73:790-805.

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