نوع مقاله : علمی پژوهشی
نویسندگان
1 دانش‌آموخته کارشناسی‌ارشد، گروه بیوتکنولوژی کشاورزی، دانشگاه پیام نور، تهران، ایران
2 استادیار بخش بیوتکنولوژی‌گیاهی، پژوهشگاه ملی مهندسی ژنتیک و زیست‌فناوری، تهران، ایران
3 استاد، گروه بیوتکنولوژی کشاورزی، دانشگاه پیام نور، تهران، ایران
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Acetyl transferases are enzymes responsible for enzymatic transfer of an acetyl group to suitable receptor molecule by using acetyl CoA as donor. Acetyltransferase reaction is involved in biosynthesis pathway of some important secondary metabolites such as antibiotics, as well as in their detoxification. Trichothecenes are significant secondary metabolites produced by the plant fungal phatogens Fusarium ssp. Such as F. sporotrichioides and F. graminearums. These fungi possess specific genes in their genomes encodeinge acetyl transferase enzymes are affecting trichothecene. In this study, the gene encoding acetyltransferase from the fungus F. sporotrichioides, TRI 101, was cloned and transferred into tobacco plant as model plants and the effect of the enzyme on the detoxification of deoxynivalenol (DON), a well known trichthecene, was investigated. In addition it was shown that, in comparison whit the roots of wild type plants, transgenic roots grew normally in the deoxynivalenol-contained medium.
کلیدواژه‌ها [English]
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