اثرات الیسیتور نانوذرات نقره و تنش خشکی بر بیان ژن بتاکاروتن هیدروکسیلاز (bch) بر عملکرد کارتنوئید زعفران (Crocus sativus L.)

نوع مقاله : علمی پژوهشی

نویسندگان

1 کارشناسی‌ارشد، گروه اصلاح‌نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل

2 دانشیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل

3 استادیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل

4 استادیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه بیرجند

چکیده

این پژوهش به‌منظور بررسی تأثیر تنش خشکی و نانو ذرات نقره بر بیان ژن بتاکاروتن‌هیدروکسیلاز (bch) و تولید کارتنوئید در گیاه زعفران انجام شد. آزمایش در دو سطح آبیاری نرمال و تنش خشکی کامل بر روی نه اکوتیپ زعفران در سه سطح، شاهد (آب مقطر)، 55 و 110 پی‌پی‌ام نانو ذرات نقره پیاده شد. این بررسی به‌صورت اسپیلت پلات فاکتوریل در قالب طرح بلوک‌های کامل تصادفی در دو تکرار در مزرعه تحقیقاتی دانشگاه بیرجند و پژوهشکده زیست‌فناوری دانشگاه زابل انجام شد. بعد از تهیه نمونه‌های برگی از تمامی تیمار‌ها، استخراج RNA، ساخت cDNAو تعیین شیب دمایی، جهت بررسی الگوی بیان ژن از واکنش Real Time PCR استفاده شد. سپس داده‌ها با نرم‌افزارهای GenEX و SAS 9.2 مورد تجزیه‌ و تحلیل قرار گرفتند. اثرات اصلی تیمارهای اکوتیپ‌، نانوذره نقره و تنش خشکی و اثرات متقابل آن‌ها برای بیان ژن bch و میزان کارتنوئید در سطح احتمال یک درصد معنی‌دار شد. حداکثر بیان ژن bch و مقدار کارتنوئید در سطح 55 پی‌پی‌ام نانوذره نقره تحت تنش خشکی در اکوتیپ قاین (به ترتیب 62/1478 و 37/21 میکروگرم بر گرم وزن‌تر) مشاهده شد. در نتیجه، تنش خشکی و نانوذره نقره تا سطح 55 پی‌پی‌ام موجب افزایش بیان ژن bch و میزان تولید کارتنوئید شده است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effects of silver nanoparticles elicitor and drought stress on the expression of beta -carotene hydroxylase (bch) gene on the yield of saffron carotenoid (Crocus sativus L.)

نویسندگان [English]

  • Batool Sabertanha 1
  • Baratali Fakheri 2
  • nafise Mahdinezhad 3
  • Zohre Alizade 4
1 M.Sc., Department of Plant Breeding and Biotechnology, College of Agriculture, University of Zabol, Iran.
2 Associate Professor, Department of Plant Breeding and Biotechnology, College of Agriculture, University of Zabol, Iran.
3 Assistant Professors, Department of Plant Breeding and Biotechnology, College of Agriculture, University of Birjand, Iran
4 Assistant Professors, Department of Plant Breeding and Biotechnology, College of Agriculture, University of Birjand, Iran
چکیده [English]

This study aimed to investigate the effect of drought stress and silver nanoparticles on beta-carotene hydroxylase (bch) gene expression and carotenoid production in saffron. The experiment was implemented in two levels including normal irrigation and full drought stress on nine ecotypes of saffron in three levels: control (distilled water), 55 and 110 ppm of silver nanoparticles levels. This study was conducted in split plot factorial experiment based on randomized complete block design with two replications at research field of Birjand University and biotechnology institute of Zabol University.In order to examine gene expression pattern, after taking leaf samples from all treatments, RNA extraction, cDNA synthesis and determination of temperature gradient, Real time polymerase chain reaction (Real-Time PCR) was used. Then, Data were analyzed using EX and SAS 9.2 software. The main effects of treatments with nine ecotypes of saffron, silver nanoparticle and drought stress and their interaction effects for bch gene expression and amount of carotenoid were significant at 1% probability level. The maximum bch gene expression and the amount of carotenoid was observed in 55 ppm of silver nanoparticles under drought stress in Ghaen ecotype (1478.62 & 21.37 µg.g-1, respectively). Therefore, drought stress and silver Nanoparticles up to 55ppm increased bch gene expression and carotenoid production.

کلیدواژه‌ها [English]

  • Saffron
  • Beta-carotene hydroxylase
  • nanoparticles
  • Real time PCR

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