بررسی بیان نسبی برخی از ژن‌های مسیر تنفس نوری در واکنش به تنش خشکی در کلزا (Brassica napus)

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

2 دانشیار، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

3 استادیار، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

چکیده

خشکی یکی از مخرب‌ترین تنش‌‌های محیطی مؤثر بر فرآیندهای متابولیکی گیاه است. در تنش خشکی ژن‌های بسیاری از جمله ژن‌های مسیر تنفس نوری در گیاهان متأثر می‌شوند. در این مطالعه تأثیر تنش خشکی بر بیان نسبی ژن‌های پراکسی‌زومی GO Glycolate) oxidase) و HPR1 (Hydroxy pyruvate reductase) و ژن‌های میتوکندریایی GDC (Glycine decarboxylase) و SHMT (Serine hydroxy methyl transferase) دو ژنوتیپ حساس (Hayola308) و متحمل (SLM046) کلزا (Brassica napus) در شرایط تنش (قطع آبیاری قبل از مرحله گلدهی) و بدون تنش بررسی شد. نتایج حاصل از Real time-PCR نشان داد میزان بیان نسبی ژن GO در 48، 72 و 96 ساعت پس از تنش در ژنوتیپ Hayola308 بیش‌تر از ژنوتیپ SLM046 بود. بیش‌ترین میزان بیان نسبی ژن GDC در ژنوتیپ Hayola308 در 48 ساعت پس از تنش مشاهده شد و با افزایش زمان تنش کاهش یافت. بیان نسبی ژن SHMT در 24 ساعت پس از تنش در هر دو ژنوتیپ Hayola308 و SLM046 در بیش‌ترین حد خود بود و با افزایش زمان تنش در ژنوتیپ SLM046 به‌طور ناگهانی و در ژنوتیپ Hayola308 به طور تدریجی کاهش یافت. بیش‌ترین میزان بیان نسبی ژن HPR1 در 24 ساعت پس از تنش در ژنوتیپ SLM046 بود و سپس در 48 ساعت پس از تنش به شدت کاهش یافت. به نظر می‌رسد در ساعات ابتدایی تنش، تنفس نوری در ژنوتیپ SLM046 افزایش یافته بود و با تداوم تنش، بر خلاف ژنوتیپ Hayola308 نسبت به شرایط موجود سازگاری یافته و تنفس نوری را مهار کرده است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The investigation of some photorespiration genes relative expression in response to drought stress in canola (Brassica napus)

نویسندگان [English]

  • Maryam Pasandide arjmand 1
  • Habibollah Samizadeh Lahiji 2
  • Mohammad Mohsenzadeh Golfazani 3
1 M.Sc. student, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
2 Associate Professor, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
3 Assistant Professor. Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
چکیده [English]

Drought is one of the most devastating environmental stresses that adversely affect plant metabolic processes. Many plant genes such as photorespiration ones are involve in response to drought stress. In the present study, the effects of drought stress on the expression level of two peroxisomal (Hydroxy pyruvate reductase (HPR1) and Glycolate oxidase (GO)) and two mitochondrial (Serine hydroxy methyl transferase (SHMT) and Glycine decarboxylase (GDC)) genes were studied in susceptible (Hayola308) and tolerant (SLM046) genotypes of canola (Brassica napus) under stress (irrigation cut at flowering stage) and non-stress conditions. The result of real time-PCR showed that in Hyola308 genotype the expression level of GO gene at 48, 72 and 96 hours after stress was higher than SLM046 genotype. In Hyola308 genotype, the highest expression level of GDC gene observed at 48 hours of stress and then decreased. The highest relative expression level of SHMT gene in both Hyola308 and SLM046 genotypes detected at 24 hours after stress and then in SLM046 genotype, its level decreased at 48 hours after stress, while in Hyola308 genotype, its expression declined over the time of exposure to stress. SLM046 genotype showed highest amount of HPR1 expression level at 48 hours after stress. It seems that the expression of photorespiration genes in SLM046 genotype increased at the initial times of exposure to stress and with continue the stress, it showed more adaptation to stress and control the photorespiration unlike Hyola308.

کلیدواژه‌ها [English]

  • Drought stress
  • GDC
  • GO
  • HPR1
  • Investigation of genes expression

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