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بررسی بیان نسبی برخی از ژن‌های مسیر تنفس نوری در واکنش به تنش خشکی در کلزا (Brassica napus)

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

2 دانشیار، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

3 استادیار، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

چکیده

خشکی یکی از مخرب‌ترین تنش‌‌های محیطی مؤثر بر فرآیندهای متابولیکی گیاه است. در تنش خشکی ژن‌های بسیاری از جمله ژن‌های مسیر تنفس نوری در گیاهان متأثر می‌شوند. در این مطالعه تأثیر تنش خشکی بر بیان نسبی ژن‌های پراکسی‌زومی GO Glycolate) oxidase) و HPR1 (Hydroxy pyruvate reductase) و ژن‌های میتوکندریایی GDC (Glycine decarboxylase) و SHMT (Serine hydroxy methyl transferase) دو ژنوتیپ حساس (Hayola308) و متحمل (SLM046) کلزا (Brassica napus) در شرایط تنش (قطع آبیاری قبل از مرحله گلدهی) و بدون تنش بررسی شد. نتایج حاصل از Real time-PCR نشان داد میزان بیان نسبی ژن GO در 48، 72 و 96 ساعت پس از تنش در ژنوتیپ Hayola308 بیش‌تر از ژنوتیپ SLM046 بود. بیش‌ترین میزان بیان نسبی ژن GDC در ژنوتیپ Hayola308 در 48 ساعت پس از تنش مشاهده شد و با افزایش زمان تنش کاهش یافت. بیان نسبی ژن SHMT در 24 ساعت پس از تنش در هر دو ژنوتیپ Hayola308 و SLM046 در بیش‌ترین حد خود بود و با افزایش زمان تنش در ژنوتیپ SLM046 به‌طور ناگهانی و در ژنوتیپ Hayola308 به طور تدریجی کاهش یافت. بیش‌ترین میزان بیان نسبی ژن HPR1 در 24 ساعت پس از تنش در ژنوتیپ SLM046 بود و سپس در 48 ساعت پس از تنش به شدت کاهش یافت. به نظر می‌رسد در ساعات ابتدایی تنش، تنفس نوری در ژنوتیپ SLM046 افزایش یافته بود و با تداوم تنش، بر خلاف ژنوتیپ Hayola308 نسبت به شرایط موجود سازگاری یافته و تنفس نوری را مهار کرده است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The investigation of some photorespiration genes relative expression in response to drought stress in canola (Brassica napus)

نویسندگان [English]

  • Maryam Pasandide arjmand 1
  • Habibollah Samizadeh Lahiji 2
  • Mohammad Mohsenzadeh Golfazani 3

1 M.Sc. student, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Associate Professor, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

3 Assistant Professor. Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

چکیده [English]

Drought is one of the most devastating environmental stresses that adversely affect plant metabolic processes. Many plant genes such as photorespiration ones are involve in response to drought stress. In the present study, the effects of drought stress on the expression level of two peroxisomal (Hydroxy pyruvate reductase (HPR1) and Glycolate oxidase (GO)) and two mitochondrial (Serine hydroxy methyl transferase (SHMT) and Glycine decarboxylase (GDC)) genes were studied in susceptible (Hayola308) and tolerant (SLM046) genotypes of canola (Brassica napus) under stress (irrigation cut at flowering stage) and non-stress conditions. The result of real time-PCR showed that in Hyola308 genotype the expression level of GO gene at 48, 72 and 96 hours after stress was higher than SLM046 genotype. In Hyola308 genotype, the highest expression level of GDC gene observed at 48 hours of stress and then decreased. The highest relative expression level of SHMT gene in both Hyola308 and SLM046 genotypes detected at 24 hours after stress and then in SLM046 genotype, its level decreased at 48 hours after stress, while in Hyola308 genotype, its expression declined over the time of exposure to stress. SLM046 genotype showed highest amount of HPR1 expression level at 48 hours after stress. It seems that the expression of photorespiration genes in SLM046 genotype increased at the initial times of exposure to stress and with continue the stress, it showed more adaptation to stress and control the photorespiration unlike Hyola308.

کلیدواژه‌ها [English]

  • Drought stress
  • GDC
  • GO
  • HPR1
  • Investigation of genes expression
مراجع
Abbaspour H, Rezaei H (2014) Effects of gibberellic effects of gibberellic acid on Hill reaction, photosynthetic pigment and phenolic compounds in Moldavian dragonhead (Dracocephalum moldavica L.) in different drought stress levels. Plant Researches (Iranian Journal of Biology). 27(5): 893-903.
Aliakbari M, Razi H (2013) Isolation of Brassica napus MYC2 gene and analysis of its expression in response to water deficit stress. Molecular Biology Research Communications. 2(3): 63-71.
Chowdhury SR, Choudhuri MA (1985) Hydrogen peroxide metabolism as an index of water stress tolerance in jute. Physiologia Plantarum. 65(4): 476-480.
Corpas FJ, Barroso JB, del Rı́o LA (2001) Peroxisomes as a source of reactive oxygen species and nitric oxide signal molecules in plant cells. Trends in Plant Science. 6(4): 145-150.
Cui LL, Lu YS, Li Y, Yang C, Peng XX (2016) Overexpression of glycolate oxidase confers improved photosynthesis under high light and high temperature in rice. Frontiers in Plant Science. 7: 1-12
Dorak M (2007) Real-time PCR. Taylor, Francis Group Press. 333.
Fletcher RS, Herrmann D, Mullen JL, Li Q, Schrider DR, Price N, Lin J, Grogan K, Kern A, McKay JK (2016) Identification of polymorphisms associated with drought adaptation QTL in Brassica napus by resequencing. G3: Genes. Genomes. Genetics. 6(4): 793-803.
Ito Y, Katsura K, Maruyama K, Taji T, Kobayashi M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2006) Functional analysis of rice DREB1/CBF-type transcription factors involved in cold-responsive gene expression in transgenic rice. Plant and Cell Physiology. 47(1): 141-153.
Jamali A, Salomé PA, Schilling SH, Weber AP, McClung CR (2009) Arabidopsis photo respiratory serine hydroxymethyl transferase activity requires the mitochondrial accumulation of ferredoxin-dependent glutamate synthase. The Plant Cell. 21(2): 595-606.
Kido EA, Neto JRC, LO Silva R, Pandolfi V, Guimaraes ACR, Veiga DT, Chabregas SM, Crovella S, Benko-Iseppon AM (2012) New insights in the sugarcane transcriptome responding to drought stress as revealed by supersage. The Scientific World. 2012: 1-14.
Kim YO, Pan S, Jung CH, Kang H, (2007) A zinc finger-containing glycine-rich RNA-binding protein, atRZ-1a, has a negative impact on seed germination and seedling growth of Arabidopsis thaliana under salt or drought stress conditions. Plant and Cell Physiology. 48(8): 1170-1181.
Leegood RC, Lea PJ, Adcok MD, Häusler RE (1995) The regulation and control of photorespiration. Experimental Botany. 46: 1397-1414.
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. methods, 25(4): 402-408.
Maier A, Fahnenstich H, V Caemmerer S, Engqvist MK, Weber AP, Flügge UI, Maurino VG (2012) Transgenic introduction of a glycolate oxidative cycle into A. thaliana chloroplasts leads to growth improvement. Frontiers in Plant Science. 3(38): 1-12.
Marmagne A, Brabant P, Thiellement H, Alix K (2010) Analysis of gene expression in resynthesized Brassica napus allotetraploids: transcriptional changes do not explain differential protein regulation. New Phytologist. 186(1): 216-227.
Mirzaee M, Moieni A, Ghanati F (2013) Effects of drought stress on the lipid peroxidation and antioxidant enzyme activities in two canola (Brassica napus L.) cultivars. Agricultural Science and Technology. 15(3): 593-602.
Mittler R, Zilinskas BA (1994) Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought stress and following recovery from drought. The Plant. 5(3): 397-405.
Moreno JI, Martín R, Castresana C (2005) Arabidopsis SHMT1, a serine hydroxymethyltransferase that functions in the photorespiratory pathway influences resistance to biotic and abiotic stress. The Plant. 41(3):451-463.
Nakabayashi R, Yonekura‐Sakakibara K, Urano K, Suzuki M, Yamada Y, Nishizawa T, Matsuda F, Kojima M, Sakakibara H, Shinozaki K, Michael AJ (2014) Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids. The Plant. 77(3): 367-379.
Navabpour S (2013) Induced genes expression pattern in response to drought stress in repeseed (Brassica napus). Seed and Plant Improvement. 29(3): 535-549.
Ogren WL (1984) Photorespiration: pathways, regulation and modification. Annual Review of Plant Physiology and Plant Molecular Biology. 35: 415-442.
Parry MA, Andralojc PJ, Khan S, Lea PJ, Keys AJ (2002) Rubisco activity: effects of drought stress. Annals of Botany. 89(7): 833-839.
Pirzad A, Jalilian J, Akbari-Bavandi V (2015) Improving grain yield of mung bean (Vigna radiata L.) using zeolite under water deficit conditions. Research in Field Crops. 3(1): 1-13.
Rizhsky L, Liang H, Mittler R (2002) The combined effect of drought stress and heat shock on gene expression in tobacco. Plant Physiology. 130(3): 1143-1151.
Schmid M, Davison TS, Hen SR, Pape UJ, Demar M, Vingron M, Schölkopf B, Weigel D, Lohmann JU (2005) A gene expression map of Arabidopsis thaliana development. Nature Genetics. 37(5): 501-506.
Serres J, Mittler R (2006) The roles of reactive oxygen species in plant cells. Plant Physiology. 141 (2311):311-311.
Shinozaki K, Yamaguchi-Shinozaki K (2007) Gene networks involved in drought stress response and tolerance. Experimental Botany. 58(2): 221-227.
Talame V, Ozturk NZ, Bohnert HJ, Tuberosa R (2007) Barley transcript profiles under dehydration shock and drought stress treatments: a comparative analysis. Experimental Botany, 58: 229-240.
Timm S, Mielewczik M, Florian A, Frankenbach S, Dreissen A, Hocken N, Fernie AR, Walter A, Bauwe H (2012a) High-to-low CO2 acclimation reveals plasticity of the photorespiratory pathway and indicates regulatory links to cellular metabolism of Arabidopsis. PLoS One. 7(8): 1-15.
Timm S, Florian A, Arrivault S, Stitt M, Fernie AR, Bauwe H (2012b) Glycine decarboxylase controls photosynthesis and plant growth. Federation of European Biochemical Societies. 586(20): 3692-3697.
Tolbert NE (1997) The C2 oxidative photosynthetic carbon cycle. Annual Review of Plant Biology, 48(1): 1-25.
Wang N, Qian W, Suppanz I, Wei L, Mao B, Long Y, Meng J, Müller AE, Jung C (2011) Flowering time variation in oilseed rape (Brassica napus L.) is associated with allelic variation in the FRIGIDA homologue BnaA. FRI. a. Experimental Botany. 62(15): 5641-5658
Wingler A, Quick WP, Bungard RA, Bailey KJ, Lea PJ, Leegood RC (1999) The role of photorespiration during drought stress: an analysis utilizing barley mutants with reduced activities of photorespiratory enzymes. Plant, Cell & Environment. 22(4): 361-373.
Yang B, Srivastava S, Deyholos MK, Kav NN (2007) Transcriptional profiling of canola (Brassica napus L.) responses to the fungal pathogen Sclerotinia sclerotiorum. Plant Science. 173(2): 156-171.
 
 
فصلنامه علمی زیست فناوری گیاهان زراعی
دوره 6، شماره 1 - شماره پیاپی 17
خرداد 1396
صفحه 31-42
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