تأثیر کاربرد سالیسیلیک‌اسید در القاء مقاومت گندم به بیماری سپتوریای برگی (STB) با عامل Zymoseptoria tritici

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی دکتری بیماری‌شناسی گیاهی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران

2 دانشیار گروه بیماری‌شناسی گیاهی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران

3 دانشیار موسسه تحقیقات اصلاح و تهیه نهال و بذر، سازمان تحقیقات، آموزش و نرویج کشاورزی، کرج

4 استادیار پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری، تهران

چکیده

این تحقیق به منظور بررسی تأثیر سالیسیلیک اسید روی کاهش شدت بیماری سپتوریای برگی گندم (STB) در سه رقم تتراپلوئید و سه رقم هگزاپلوئید، در شرایط کنترل شده در مرحله گیاهچه‌ای انجام گرفت. گیاهچه‌های 10 روزه گندم ابتدا با سالیسیلیک‌ اسید به غلظت‌های صفر (شاهد)، یک، دو و چهار میلی‌مولار با مه‌پاش دستی تیمار شدند. پس از گذشت 24 ساعت، گیاهچه‌ها با استفاده از سوسپانسیون اسپور قارچ به غلظت 107 اسپور بر میلی‌لیتر مایه‌زنی شدند. ارزیابی بیماری 21 روز پس از مایه‌زنی بر اساس تخمین درصد سطح برگی با لکه‌های بافت مرده حاوی پوشش پیکنیدی انجام گرفت. نتایج تجزیه واریانس داده‌ها نشان داد بین سطوح مختلف سالیسیلیک‌اسید از نظر کاهش شدت بیماری و تأثیر متقابل غلظت- رقم اختلاف معنی‌دار وجود دارد. به طوری که سالیسیلیک اسید درکاهش شدت بیماری در هر دو گروه ارقام تتراپلوئید و هگزاپلوئید مؤثر بوده و تیمار یک میلی‌مولار بیشترین تأثیر در کاهش شدت بیماری را نشان داد. در غلظت‌های دو و چهار میلی‌مولار کاهش شدت بیماری معنی دار بود اما میزان اثربخشی سالیسیلیک اسید در القاء مقاومت کمتر از غلظت یک میلی‌مولار بود. در مجموع این نتایج نشان داد که فعال کردن سیستم‌های دفاعی گیاه میزبان با استفاده از سالیسیلیک اسید می‌تواند نقش قابل توجهی در القاء مقاومت اکتسابی سیستمیک علیه بیماری سپتوریای برگی در گندم باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of salicylic acid application on the reduction of wheat septoria leaf blotch (STB) symptoms causing by Zymoseptoria tritici

نویسندگان [English]

  • Azadeh Ghaneie 1
  • Naser Safaie 2
  • Rahim Mehrabi 3
  • Forough Sanjarian 4
1 Ph.D. Candidate of Plant Pathology, respectively, Tarbiat Modares University, Tehran, Iran.
2 Associate Professor, respectively, Tarbiat Modares University, Tehran, Iran.
3 Associate Professor, Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
4 Assistant Professor, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
چکیده [English]

This study was carried out to evaluate the effect of salicylic acid on the reduction of disease severity of septoria tritici blotch (STB) in three tetraploeid and three hexaploeid wheat cultivars, under controlled conditions at seedling stage. The 10-day-old seedlings were initially treated with 0 (control), 1, 2 and 4 mM of salicylic acid with a handy sprayer. After 24 hrs, the seedlings were inoculated with fungal spore suspensions adjusted at 107 sopres/ml. The disease progress was evaluated 21 days post inoculation based on visual estimation of the percentage of leaf area with necrotic lesions bearing pycnidia. The results showed that there were significant differences on the reduction of disease severity among different concentrations of applied salicylic acid as well as among cultivar/ concentration interactions. In addition, the results showed that application of salicylic acid reduced disease severity of both tetraploid and hexaploid wheat cultivars and 1 mM salicylic acid was the most effective concentration on the reduction of disease severity. Although, the disease severity was significantly reduced by application of 2 and 4 mM salicylic acid, the effectiveness of these concentrations was less than application of 1mM. Overall, these results revealed that activation of plant defense systems through application of salicylic acid could play a significant role in systemic acquired resistance against STB disease.

کلیدواژه‌ها [English]

  • Zymoseptoria tritici
  • salicylic acid
  • Septoria leaf blotch

مراجع

Arzani A, (2004) Evaluation of genetic diversity and crop feathers of Durum wheat. Journal of Crop Production and Processing. 7: 115-127.
Abrinbana M, Mozafari J, Shams‐bakhsh M, Mehrabi R (2010) Genetic structure of Mycosphaerella graminicola populations in Iran. Plant Pathol. 59: 829-838.
Boller T, Felix G (2009) A renaissance of elicitors: perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors. Annu. Rev. Plant Biol. 60: 379-406.
Brown A, Rosielle A (1980) Prospects for control of Septoria. Journal of Agriculture 21: 8-11.
Brown JK, Chartrain, L, Lasserre-Zuber P, Saintenac C (2015) Genetics of resistance to Zymoseptoria tritici and applications to wheat breeding. Fungal Genet. Biol. 79: 33-41.
Chen Z, Zheng Z, Huang J, Lai Z, Fan B (2009) Biosynthesis of salicylic acid in plants. Plant Signal. Behav. 4: 493-496.
Conrath U, Beckers GJ, Flors V, García-Agustín P, Jakab G, Mauch F, Newman M-A, Pieterse CM, Poinssot B, Pozo MJ (2006) Priming: getting ready for battle. Mol. Plant-Microbe In. 19: 1062-1071.
Dempsey DMA, Vlot AC, Wildermuth MC, Klessig DF (2011) Salicylic acid biosynthesis and metabolism. The Arabidopsis Book:e0156.
De Vos M, Van Oosten VR, Van Poecke RM, Van Pelt JA, Pozo MJ, Mueller MJ, ... Dicke M (2005). Signal signature and transcriptome changes of Arabidopsis during pathogen and insect attack. Mol. Plant Microbe Interact. 18: 923-937.
Fayez KA, Bazaid SA (2014). Improving drought and salinity tolerance in barley by application of salicylic acid and potassium nitrate. J. Saudi Soci. Agricultural Sci. 13: 45-55.
Freier B, Boller EF (2009) Integrated pest management in Europe–history, policy, achievements and implementation. In:  Integrated pest management: dissemination and impact. Springer, Netherlands, pp 435-454.
Friedrich L, Vernooij B, Gaffney T, Morse A, Ryals J (1995) Characterization of tobacco plants expressing a bacterial salicylate hydroxylase gene. Plant mol. Bio. 29: 959-968.
Garcia C, Marshall D (1992) Observations on the ascogenous stage of Septoria tritici in Texas. Mycol. Res. 96: 65-70.
Ghaneie A, Mehrabi R, Safaie N, Abrinbana M, Saidi A, Aghaee M (2012) Genetic variation for resistance to septoria tritici blotch in Iranian tetraploid wheat landraces. Eur. J. plant path. 132: 191-202.
Gholamnejad J, Mohammadi-Goltapeh E, Sanjarian F, Safaie N, and Razavi K (2013) Study of Effect of salicylic acid on diseases symptom of Septoria tritici blotch (STB). Research in Plant Pathology, 2: 35-46. (in Farsi with English abstract).
Gilbert J, Woods S, Tekauz A (1998) Relationship between environmental variables and the prevalence and isolation frequency of leaf-spotting pathogens in spring wheat. Can. J. plant pathol. 20: 158-164.
Glazebrook J (2005) Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annu. Rev. Phytopathol. 43: 205-227.
Hadi M, Balali G (2010) The effect of salicylic acid on the reduction of Rhizoctonia solani damage in the tubers of marfona potato cultivar. Am. Eurasian J. Agric. Environ. Sci. 7: 492-496.
Hekmati Zh, Aalami A, Sohani MM (2014) Effect of salicylic acid on expression of some defense-related genes in rice. Iranian Genetic society 9: 363-373. (in Farsi)
Jones JD, Dangl JL (2006) The plant immune system. Nature 444: 323-329
Király L, Künstler A, Bacsó R, Hafez Y, Király Z (2013) Similarities and differences in plant and animal immune systems-what is inhibiting pathogens? Acta Phytopathol. Entomol. Hung. 48: 187-205.
Kema G, Annone J, Sayoud R, Van Silfhout C, Van Ginkel M, De Bree J (1996) Genetic variation for virulence and resistance in the wheat-Mycosphaerella graminicola pathosystem. I: Interactions between pathogen isolates and host cultivars. Phytopathol 86: 200-212.
Linde C, Zhan J, McDonald B (2002) Population structure of Mycosphaerella graminicola: from lesions to continents. Phytopathol 92: 946-955.
Loughman R, Thomas G (1992) Fungicide and cultivar control of Septoria diseases of wheat. Crop Prot. 11: 349-354.
Makhdoumi M, Mehrabi R, Arshad Y (2014) Identification of Resistance Sources to Septoria tritici Blotch in Iranian Wheat Landraces. Seed and Plant Improvment Journal 30: 561-572. (in Farsi)
Maxson-Stein K, He S-Y, Hammerschmidt R, Jones AL (2002) Effect of treating apple trees with acibenzolar-S-methyl on fire blight and expression of pathogenesis-related protein genes. Plant Dis. 86: 785-790
McDonald B, Pettway R, Chen R, Boeger J, Martinez J (1995) The population genetics of Septoria tritici (teleomorph Mycosphaerella graminicola). Can. J. Bot. 73: 292-301.
Nie X (2006) Salicylic acid suppresses Potato Virus Y Isolate N: O-induced symptoms in tobacco plants. Phytopathol. 96: 255-263.
Pastor V, Luna E, Mauch-Mani B, Ton J, Flors V (2013) Primed plants do not forget. Environ. Ex. Bot. 94: 46-56.
Petrak F, Esfandiari E (1941) Contributions to the knowledge of the Iranian fungus flora. Ann. Mycol. 39: 204-228.
Qi P-F, Johnston A, Balcerzak M, Rocheleau H, Harris LJ, Long X-Y, Wei Y-M, Zheng Y-L, Ouellet T (2012) Effect of salicylic acid on Fusarium graminearum, the major causal agent of fusarium head blight in wheat. Fungal biology 116: 413-426.
Quaedvlieg W, Kema G, Groenewald J, Verkley G, Seifbarghi S, Razavi M, Gohari AM, Mehrabi R, Crous P (2011) Zymoseptoria gen. nov.: a new genus to accommodate Septoria-like species occurring on graminicolous hosts. Persoonia-Molecular Phylogeny and Evolution of Fungi 26: 57-69.
Raskin I, Skubatz H, Tang W, Meeuse BJ (1990) Salicylic acid levels in thermogenic and non-thermogenic plants. Ann. Bot-London 66: 369-373.
Sakhabutdinova A, Fatkhutdinova D, Bezrukova M, Shakirova F (2003) Salicylic acid prevents the damaging action of stress factors on wheat plants. Bulg. J. Plant Physiol. 21: 314-319.
Sanderson F (1972) A Mycosphaerella species as the Ascogenous state of Septoria tritici Rob. and Desm. New Zeal. J. Bot. 10: 707-709.
Sarahinobar, M., Jahedi, A., Safaie, N., Niknam, V., Ebrahimzadeh, H. 2015. Dual functional role of Salicylic acid against Fusarium; long lasting priming and direct immediate effect. Intl. J. Farm. And Alli. Sci. 4: 442-447.
Sarwar N, Ch Z, Hayat M, Haq I (2010) Seed treatments induced systemic resistance in chickpea against fusarium wilt in wilt sick field. Pak. J. Bot. 42: 3323-3326.
Shakirova F, Bezrukova M (1997) Induction of Wheat Resistance against Environmental Salinization by Indolylacetic Acid. Biology bulletin-Russian academy of sciences c/c of izvestiia-rossiiskoi akademii nauk seriia biologycheskaia 24: 109-112.
Sprague R (1950) Diseases of cereals and grasses in North America. Diseases of cereals and grasses in North America. The Ronald Press Company, New York, pp 80-324.
Tahermazandarani F, Mehrabi R, Maleki M (2014) Effectiveness of Septoria tritici Blotch (Stb) Resistance Genes to Mycosphaerella graminicola Isolates Collected from Fars Province. Seed and Plant Improvment Journal 30: 669-682. (in Farsi)
Thomma BP, Nürnberger T, Joosten MH (2011) of PAMPs and effectors: the blurred PTI-ETI dichotomy. The Plant Cell 23: 4-15.
Tsuda K, Katagiri F (2010). Comparing signaling mechanisms engaged in pattern-triggered and effector-triggered immunity. Curr. Opin. Plant. Biol. 13: 459-465.
Vlot AC, Dempsey DMA, Klessig DF (2009) Salicylic acid, a multifaceted hormone to combat disease. Ann. Rev. Phytopathol 47: 177-206.
Zamani E, Sanjarian F, Mohamadi-Goltapeh E, Safaei N (2016) Studying the resistance of wheat seedlings grown from treated seeds with salicylic acid against Mycosphaerella graminicola. Plant Protection (Scientific Journal of Agriculture) 39: 1-14. (in Farsi with English abstract)

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