مطالعه ارتباط ژن TSA1 با مسیر بیوسنتز گلوکوزینولات ها ”ترکیبات ثانویه سولفوردار در گیاهان خانواده کلم”

نوع مقاله : علمی پژوهشی

نویسندگان

1 استادیار گروه زیست شناسی سلولی و مولکولی، دانشکده علوم پایه، دانشگاه مازندران، بابلسر، ایران

2 استادیار گروه زیست شناسی، دانشکده علوم پایه، دانشگاه مازندران، بابلسر، ایران

3 استاد گروه فیزیولوژی گیاهی، دانشکده علوم طبیعی و مهندسی، دانشگاه گرونینگن، گرونینگن، هلند

چکیده

اهمیت تغذیه‌ای گلوکوزینولات‌ها در انسان‌ها، حیوانات و اثرات آنها در بهبود سرطان، بیماری‌های قلبی-عروقی، عصبی و نقش دفاعی آنها درگیاهان علیه آفت‌ها و پاتوژن‌ها موجب گردیده است تا مسیر بیوسنتز گلوکوزینولات‌ها گزینه مناسبی جهت مطالعات ژنتیکی قرار گیرند. در شرایط تنشی، بیوسنتز گلوکوزینولات‌ها به‌وسیله مجموعه‌ای از فاکتورهای رونویسی تحت کنترل مثبت یا منفی قرار می‌گیرند. شبه رسپتورهای کینازی در واقع پروتئین‌هایی هستند که به عنوان گیرنده‌های سطحی سلول، پیام‌های محیطی را دریافت می‌کنند. هدف از تحقیق حاضر مطالعه نقش تنظیمی ژن AT2G37050 در ارتباط با بیوسنتز گلیکوزینولاتها می‌باشد. به دنبال مطالعات پروتئومیک قبلی انجام شده بر روی گیاه آرابیدوپسیس تالیانای جهش‌یافته که ژن شبه رسپتور کینازی AT2G37050 آن از کار افتاده، مشخص گردید که 22 پروتئین در گیاه جهش‌یافته وجود دارند درحالی که در گیاه نوع وحشی (کنترل) حضور ندارند. مطالعات بیوانفورماتیکی حاضر، برگرفته از منابع مستخرج از الگوریتم GeneMANIA به کمک نرم‌افزار Cytoscape نشان داده است که بین سه پروتئین TSK-associating protein1 (TSA1)،AT3G47570 و AT1G08750 با پروتئین های درگیر در بیوسنتز گلوکوزینولات‌ها و همچنین تنظیم‌کننده‌های بیوسنتز گلوکوزینولات‌ها ارتباطات بیولوژیکی از نوع هم بیانی ژنی، برهم کنش پروتئین- پروتئین و قرار گیری در یک مکان مشترک درون سلولی (هم مکانی) وجود دارد. از طرفی دیگر، با توجه به اینکه گلوکوزینولات‌ها به عنوان ترکیبات ثانویه سولفوردار در آرابیدوپسیس تالیانا و گیاهان خانواده کلم شناخته شده‌اند، وجود ارتباط زیستی بین ژن TSA1 و AT3G47570 با ژن‌ها/ پروتئین‌های ناقل سولفور و مسیر احیای سولفور، بر نقش این دو ژن در ارتباط با بیوسنتز گلوکوزینولات‌ها قوت بیشتری بخشیده است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Relation between TSA1 gene and biosynthesis of glucosinolates ”secondary sulfur compounds in Brassicaceae family”

نویسندگان [English]

  • Omid Jazayeri 1
  • Tahereh Aghajanzadeh 2
  • Theo Elzenga 3
1 Assistant Professor, Department of Molecular and Cell Biology, Faculty of Basic Science, University of Mazandaran, Babolsar, Iran.
2 Assistant Professor, Department of Biology, Faculty of Basic Science, University of Mazandaran, Babolsar, Iran.
3 Professor, Laboratory of Plant Physiology, Faculty of Science and Engineering, University of Groningen, Groningen, the Netherlands.
چکیده [English]

Glucosinolates are a potential target for genetic manipulation in crop improvement programs, due to their diverse roles in animal nutrition, plant defense against pests and pathogens, beneficial treatment effects in cancer, cardiovascular and neurological diseases. To date, more than 30 genes which are involved in biosynthesis of glucosinolates have been identified in Arabidopsis thaliana. During biotic and abiotic stresses, glucosinolate biosynthesis is further controlled by a complex network of transcription factors. Receptor-like kinases (RLKs) are proteins which act as cell surface receptors perceiving developmental and environmental signals in plants. Following functional studies of a RLKs (AT2G37050) in Arabidopsis thaliana, our previous proteomic data showed that 22 proteins such as TSA1, AT3G47570 and AT1G08750 were appeared in knockout of AT2G37050 while these proteins were not detected in wild type plant (unpublished data). The analysis resulted from GeneMANIA algorithm revealed biological connections between these three genes and glucosinolate biosynthesis pathway genes as well as regulating genes of glucosinolate biosynthesis pathway. Since glucosinolates are considered as sulfur containing secondary compounds in Arabidopsis thaliana and Brassicaceae family, biological connections between TSA1 and AT3G47570 with sulfur transporter genes as well as sulfur assimilation pathway genes will support more the role of these two genes on regulation of glucosinolates biosynthesis pathway.

کلیدواژه‌ها [English]

  • Brassica
  • Glucosinolate biosynthesis
  • TSA1 gene

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