ویرایش ژنوم در راستای تغییر رنگ گل با استفاده از تکنولوژی کریسپر

نوع مقاله : مروری

نویسندگان

1 دانشجوی دکتری بیوتکنولوژی کشاورزی، دانشکده علوم زیستی و زیست‌فناوری دانشگاه شهید بهشتی، تهران، ایران

2 گروه بیوتکنولوژی گیاهی، دانشکده علوم زیستی و زیست‌فناوری دانشگاه شهید بهشتی، تهران، ایران

چکیده

امروزه تغییر رنگ گل به دلیل اهمیت تجاری آن یکی از اهداف محققان است. با ایجاد تنوع در رنگ گلبرگ گیاهان زینتی می‌توان درآمدزایی بالایی برای کشور ایجاد کرد و راه را برای صادرات آن به سایر نقاط دنیا هموار کرد. در گذشته به روش سنتی و مهندسی ژنتیک تلاش‌هایی در راستای تغییر رنگ صورت گرفته است، اما با کندی همراه بوده است. با کشف سیستم کریسپر امکان ایجاد تغییرات هدفمند در سطح ژنوم سرعت بیشتری بخود گرفت. برای ایجاد تغییر بوسیله‌ی کریسپر باید ژن مورد نظر و ناحیه‌ هدف شناسایی شود که اینکار بوسیله‌ی ابزار بیوانفورماتیک قابل انجام است. تغییر مورد نظر از طریق طراحی gRNA اعمال می‌شود. در ادامه پروتئین طبیعی و پروتئین جهش یافته عملکردشان بررسی خواهد شد. امروزه به منظور افزایش کارایی سیستم کریسپر از روش donor DNA استفاده می‌شود. در این سیستم با عمل نوترکیبی همولوگ می‌توان کارایی کریسپر را در راستای جایگزینی ژن سالم و یا خاموشی آن افزایش داد و از ایجاد تغییرات غیر اختصاصی در ژنوم جلوگیری کرد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Genome editing for Change the color of the flower using crispr technology

نویسندگان [English]

  • Masoumeh Fallah Ziarani 1
  • Masoud Tohidfar 2
1 Ph.D. student of Plant Biotechnology, Department of Plant Biotechnology, Shahid Beheshti University, Tehran, Iran
2 Associate Professor, Department of Plant Biotechnology, College of Life Science and Biotechnology, Shahid Beheshti University, Tehran, Iran
چکیده [English]

Today, Change the color of the flower due to its commercial importance is one of the goals of the researchers. Creation of the variation in flower color of ornamental plants can be highly profitable for the country and facilitate the way for export to other parts of the world. In the past, efforts have been do in the traditional way and genetic engineering for change the color of the flower, but with slowdown. With the discovery of Crispr's system, the ability to make targeted changes at the genome level took less time. In order to target change by Crispr, the target gene and area must be identified, that is done by bioinformatic tools. The desired change through the design of gRNA is done. In following, the normal protein and mutated protein were checked for the function. Today, donor DNA is used to enhance the performance of the Crispr system. In this system, by homologous recombination can be enhanced Crisper's performance by replaces a healthy gene or knocked out and prevented the creation of non-specific changes in the genome.

کلیدواژه‌ها [English]

  • chang the color of flower
  • crispr
  • genetic enginering

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