تنظیم miR319 و ژن هدف آن MYB3 در طی بهاره سازی دو رقم گندم

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی‌ارشد بیوتکنولوژی، گروه زراعت و اصلاح‌نباتات، دانشکده کشاورزی، دانشگاه زنجان، زنجان

2 دانشیار گروه زراعت و اصلاح‌نباتات، دانشکده کشاورزی، دانشگاه زنجان، زنجان

3 دانشجوی دکتری بیوتکنولوژی کشاورزی، دانشکده کشاورزی، دانشگاه زنجان، زنجان

چکیده

بسیاری از گیاهان سازگار با اقلیم‌های سرد تنها پس از یک دوره‌ی طولانی سرما گل می‌دهند که بهاره‌سازی نامیده می‌شود. در طول زندگی غلات زمستانه، گلدهی ناشی از بهاره‌سازی تنها یک قسمت از فرآیند تولیدمثلی نبوده بلکه یک مرحله نموی مهم است که می‌تواند از گیاه در مقابل تنش‌های محیطی محافظت بکند. این فرآیند در غلاتی مثل گندم زمستانه توسط ژن‌های بهاره‌سازی و عمدتا توسط ژن‌های VRN1 وVRN2 کنترل می‌شود. اگر چه مطالعات بسیاری بر روی بهاره‌سازی در گندم گزارش شده است، اما مکانیسم مولکولی بهاره‌سازی هنوز تا حد زیادی ناشناخته است. مطالعات اخیر نشان داده است که یک کلاس از RNAهای غیرکدکننده کوچک، microRNAها (miRNAs)، نقش مهمی را در گلدهی با مشارکت در مسیرهای شناخته‌شده گلدهی ایفا می‌کند. در مطالعه حاضر، miR319 و ژن هدف موردنظر آن، فاکتور رونویسی MYB3 تحت تیمارهای بهاره‌سازی در دو رقم گندم بهاره و زمستانه مورد بررسی قرار گرفت. نتایج نشان داد بیان miR319 در طی بهاره‌سازی در هر دو رقم القا شد، اما مقدار بیان ژن miR319در رقم نورستار کاهش و در رقم بهاره‌ی باز افزایش داشت. هم چنین سطوح بیان ژن MYB3 در هر دو رقم تحت بهاره‌سازی کاهش یافت. رابطه معکوس بین بیان miR319 و ژن هدف MYB3 آن وجود داشت. این نتایج نشان دهنده پیچیدگی اثر متقابل ژنوتیپ و سطوح بیان miRNA و ژن هدف تحت تیمارهای متفاوت بهاره‌سازی بود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Regulation of miR319 and its target gene (MYB3) during vernalization in two wheat cultivars

نویسندگان [English]

  • Nooshin Ashoori 1
  • Reza Fotovat 2
  • Maryam Mortezaee 3
  • Nastaran Mehri 3
1 Former Student of Plant Biotechnology, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Iran.
2 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Iran.
3 Ph.D. candidate, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Iran.
چکیده [English]

Many plants adapted to cold climates flower only after an extended period of cold, namely vernalization. In the lifetime of a winter cereals, flowering due to vernalization is not only an essential part of the reproductive process but also a critical developmental stage that can be protect the plant against environmental stresses. This process in cereals such as winter wheat is mainly regulated by the VERNALIZATION genes, VRN1 and VRN2. Although many studies on vernalization in wheat have been reported, the molecular mechanism of vernalization is still largely unknown. Recent studies were shown that a class of small non-coding RNAs, microRNAs (miRNAs), plays a key role in flowering by integrating into the known flowering pathways. In the present study, we investigated the expression of miR319 and its target gene (MYB transcription factor) under the vernalization treatments in spring and winter wheat cultivars. Our results demonstrate that cold treatment induced the miR319 expression in both cultivars, but miR319 level is down-regulated in Norstar and up-regulated in the spring wheat cultivar Baz. Likewise, the expression levels of MYB3 gene was decreased in both cultivars exposed to vernalization. There was reverse relationship between expression of miR319 and its target gene MYB3. These results highlight the complex interactions between genotypes, miRNA and expression of target gene under different vernalization treatment.

کلیدواژه‌ها [English]

  • Cereal plants
  • Flowering genes
  • MicroRNA
  • Transcription factor
  • Vernalization

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