بررسی الگوی بیان ژن‌های عوامل رونویسی (MYB و WRKY) تحت تنش شوری در مرحله گیاهچه‌ای یونجه زراعی با استفاده از PCR در زمان واقعی

نوع مقاله : علمی پژوهشی

نویسندگان

استادیار پژوهش بخش تحقیقات اصلاح و تهیه نهال و بذر، مرکز تحقیقات کشاورزی و منابع طبیعی صفی آباد، سازمان تحقیقات، آموزش و ترویج کشاورزی، دزفول، ایران.

چکیده

یکی از عمده‌ترین مشکلات در تولید و گسترش سطح کشت یونجه در جهان و از جمله ایران، شوری است. در گیاهان شناخت کامل مکانیسم‌های تحمل و ژن‌های درگیر در شرایط تنش می‌تواند باعث بهبود تحمل تنش‌های مختلف در گیاهان زراعی با استفاده از روش‌هایی چون دستکاری ژنتیکی شود. یکی از مهم‌ترین روش‌های کنترل تنش در گیاهان، تنظیم در مرحله رونویسی ژن‌هاست. عوامل رونویسی از طریق اتصال به عناصر رونویسی در پروموتور DNA میزان بیان بسیاری از ژن‌ها را تنظیم می‌کنند و بنابراین اهمیت بسزایی در تحمل تنش شوری در گیاهان دارا می‌باشند. در این پژوهش میزان بیان 4 ژن از عوامل رونویسی MYB (MYB112 و MYB14) و WRKY (WRKY53 و WRKY70) تحت تنش شوری در بافت برگ و ریشه ژنوتیپ یزدی (به عنوان ژنوتیپ متحمل شوری) و ژنوتیپ دیابلورده (به عنوان ژنوتیپ حساس شوری) مورد مطالعه قرار گرفت. انتخاب ژن‌های مذکور بر اساس تجزیه آماری داده‌های ریزآرایه یک مطالعه مربوط به تأثیر تنش شوری بر گیاه یونجه یکساله (Medicago truncatula) بود. تنش شوری کوتاه مدت باعث ایچاد تنوع قابل ملاحظه در بیان ژن‌های مذکور در بافت برگ و ریشه دو ژنوتیپ یزدی و دیابلورده گردید. با کمک آنالیز qRT-PCR (PCR در زمان واقعی) مشخص شد که بیان بالاتر فاکتورهای رونویسی MYB112 و MYB14 تحمل بیشتر به تنش شوری را به‌همراه داشته است. این یافته می‌تواند به‌نژادگران نبات را برای استفاده از این عوامل رونویسی جهت انتخاب ژنوتیپ‌های متحمل به نمک در یونجه‌های زراعی یاری نماید.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Gene Expression Patterns of some Transcription Factors (MYB and WRKY) under Salt Stress in the seedling stage of Alfalfa using qPCR

نویسندگان [English]

  • Ahmad Ali Shoushi Dezfuli1 Shoushi Dezfuli
  • Ahmad Kalantar ahmadi
Assistant Professor Seed and Plant Improvement Institute Department, Safiabad Agricultural and Natural Resource Research Center, AREEO, Dezful, Iran.
چکیده [English]

Salinity is one the major problems for production and increasing the area under cultivation around the world and Iran. Understanding of defense mechanisms and genes involved could improve tolerance to different stresses in crops by using some methods such as genetic manipulation. Regulation in the gene transcription phase is one the most methods to control stress in plants. Transcription factors thought binding with transcription elements in DNA promoters regulate genes expression which plays a key role in tolerance to salinity stress in plants. An experiment was conducted to evaluate four genes expression of transcription factors of MYB (MYB14 and MYB112) and WRKY (WRKY53 and WRKY70) in leaf and root tissue of Yazdi genotype (tolerant genotype to salinity) and Diabloverde (sensitive genotype to salinity) under salinity stress. The selection of these genes was based on the statistical analysis of the microarray data that was related to a study on the effect of salinity stress on Medicago truncatula. Short-term salinity stress caused a significant variation in the expression of these genes in leaf and root tissues of Yazdi and Diabloverde genotypes. Real-Time PCR analysis revealed that higher expression of transcription factors (MYB112 and MYB14) associated with more tolerance to salinity stress. This finding could be assisted plant breeders to apply these transcriptional factors to choose tolerant genotypes to salinity in alfalfa.

کلیدواژه‌ها [English]

  • qRT-PCR
  • Salinity
  • MYB
  • WRKY
  • Alfalfa

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