اشباع نقشه ژنتیکی و مکان‌یابی ژن‌های کنترل کننده صفات با استفاده از خانواده‌های F3 حاصل از تلاقی بادیا×کویر

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی کارشناسی‌ارشد رشته بیوتکنولوژی کشاورزی، دانشگاه گنبد کاووس، ایران

2 دانشیار گروه تولیدات گیاهی، دانشگاه گنبد کاووس، ایران

3 استادیار گروه تولیدات گیاهی، دانشگاه گنبد کاووس، ایران

چکیده

جو (L. vulgare Hordeum) یک گیاه مدل برای پژوهش‌های ژنتیکی و فیزیولوژیکی بوده و سازگاری بالایی بـه شـرایط مختلـف نـشان می‌دهد. به منظور مکان‌یابی QTLهای ژنومی‌ کنترل‌کننده صفات زراعی جو آزمایشی در سال زراعی 1396 - 1395 با 104 خانواده به همراه والدین آنها ( بادیا و کویر) در مزرعه تحقیقاتی دانشکده گنبدکاووس در قالب طرح اگمنت اجرا گردید. بیوماس، وزن سنبله‌، تعداد سنبله، طول سنبله‌، عملکرد دانه، طول پدانکل، قطر ساقه، طول پرچم، عرض پرچم، وزن پرچم، تعداد دانه، وزن دانه، طول ریشک برای کلیه خانواده‌ها اندازه‌گیری شد. برای اشباع نقشه پیوستگی از 19 نشانگر ISSR (در مجموع93 آلل) در 7 گروه پیوستگی متعلق به 7 کروموزوم جو با سانتی‌مورگانی برابر با 5/617 و فاصله بین دو نشانگر مجاور برابر با 41/5 سانتی‌مورگان منتسب شد. برای صفات زراعی مورد بررسی در مجموع 21 جایگاه واجد QTL مکان‌یابی گردید. برای تعداد خوشه، پنج QTL روی کروموزوم‌های 3، 4، 5 و 7، برای طول سنبله دو QTLروی کروموزم‌های 4 و7، برای طول پدانکل در کروموزم 3، برای قطر ساقه دو QTL روی کروموزم‌های 1، 4، 6 و 7 برای طول پرچم با سه QTL روی کروموزم‌های 3 و4 و همچنین برای طول ریشک یک QTL بزرگ اثر ردیابی شد. QTLهای بزرگ اثر کنترل‌کننده صفات موردنظر و نشانگرهای پیوسته با آنها می‌توانند در برنامه‌های انتخاب به کمک نشانگر مورد استفاده قرار گیرند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Saturation of genetic map and locating of (QTLs) related to traits in barley using F3 families caused Badia× Kavir cross

نویسندگان [English]

  • Samira Bakhtiari 1
  • Hossein Sabouri 2
  • Mehdi Mollashahi 3
  • hossein hossein moghaddam 3
1 M.Sc. in Agricultural Biotechnology, University of Gonbad-Kavoos, Iran.
2 Associate Professor, Department of Plant Production, University of Gonbad-Kavoos, Iran.
3 Assistant Professor, Department of Plant Production, University of Gonbad-Kavoos, Iran.
چکیده [English]

Barley (Hordeum vulgare L.) is a model plant for genetical and physiological studies and has a high adaptability to different conditions. In order to locate QTL, the genomic areas controlling barley agronomic traits under experimental conditions were conducted in 104 families with their parents (Badia and Kavir) in the Research Farm of Gonbad-e-Kavous College in 2013-2014. Agronomic traits such as biomass, spike weight, spike number, spike length, grain yield, peduncle length, stem diameter, flag leaf length, flag leaf width, flag leaf weight, grain numbers, grain weight, and awn length for all families were measured. For the saturation of map, 19 (93 alleles), the ISSR marker used that assigned to 7 groups of attachment to 7 chromosomes with a genome length of 617.5 cM and the average between markers equal to 5.41 cM Morgan. A total of 21 locations with QTL were identified for the agronomic traits. Thirteen major effect QTLs that controlled a large proportion of phenotypic variation were identified. Major QTLs The effects of controlling the desired attributes and their linked markers can be used in selection programs using the marker.

کلیدواژه‌ها [English]

  • Barley
  • Agronomic traits
  • QTL
  • SSR markers
  • ISSR markers

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