تنوع ژنتیکی و شناسایی آلل‌های ریز‌ماهواره حاوی اطلاعات در گیاهچه‌های برنج تحت شرایط نرمال و تنش شوری

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی کارشناسی‌ارشد، گروه تولیدات گیاهی، دانشکده کشاورزی، دانشگاه گنبد کاووس، گنبد کاووس، ایران.

2 دانشیار، گروه تولیدات گیاهی، دانشکده کشاورزی، دانشگاه گنبد کاووس، گنبد کاووس، ایران.

3 استادیار ، گروه تولیدات گیاهی، دانشکده کشاورزی، دانشگاه گنبد کاووس، گنبد کاووس، ایران.

4 مربی، بخش دانشکده علوم پایه و فنی‌مهندسی دانشگاه گنبد کاووس، گنبد کاووس، ایران.

چکیده

تنوع ژنتیکی به‌منظور اصلاح گیاهان زراعی و ارتقای سطح مدیریت منابع ژنتیکی از اهمیت زیادی در برنامه‌های اصلاحی برخوردار است. به‌منظور مطالعه تنوع ژنتیکی 102 ژنوتیپ مختلف برنج بر اساس صفات گیاهچه‌ای و آزمایش‌های مولکولی، آزمایشی در قالب طرح کامل تصادفی در سه تکرار اجرا شد. صفاتی نظیر طول ساقه‌، طول ریشه، طول بزرگ‌ترین برگ، عرض بزرگ‌ترین برگ، تعداد ریشه‌، وزن تر ساقه، وزن تر ریشه‌ و حجم کل ریشه‌ مورد بررسی قرار گرفته و سپس، وزن خشک ساقه و وزن خشک ریشه‌ پس از قرار‌گیری در آون اندازه‌گیری شدند. میانگین محتوای اطلاعات چند شکل، 271/0 برآورد شد که نشانگر‌های RM1029 با 175/0 کم‌ترین و RM216 با 435/0 بیش‌ترین محتوای اطلاعات چند شکل را نشان دادند. نتایج تجزیه ارتباط بین نشانگرها و صفات مورد بررسی در شرایط نرمال نشان دادند که آلل RM60B برای صفت طول ریشه‌، آلل RM127A برای صفت تعداد ریشه‌، آللRM231G برای صفت وزن تر ساقه‌ درصد بالایی از تنوع فنوتیپی را توجیه نمودند. در شرایط تنش شوری آلل RM129H برای صفت طول ساقه، آللRM12091B برای صفت تعداد ریشه‌، آلل RM263G برای صفت وزن تر ساقه‌، آلل RM127C برای صفت عرض بزرگ‌ترین برگ درصد بیش‌تری از تنوع فنوتیپی را توجیه نمودند و به‌عنوان نشانگر‌های مهم شناسایی شدند. از نتایج این آزمایش می‌توان در برنامه‌های به‌نژادی برنج استفاده نمود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Genetic diversity and identification of informative microsatellite alleles in rice seedlings under normal conditions and salinity stress

نویسندگان [English]

  • Bahareh Ghasemi 1
  • Hossein Sabouri 2
  • Hossein Hossein Moghaddam 3
  • Abbas Biabani 2
  • Mohamad Javad Sheikhzadeh 4
1 Graduate Student, Department of Plant Production, Collage of Agriculture Science and Natural Resource, Gonbad Kavous University, Gonbad Kavous, Iran.
2 Associate Professor, Department of Plant Production, Collage of Agriculture Science and Natural Resource, Gonbad Kavous University, Gonbad Kavous, Iran.
3 Assistant Professor, Department of Plant Production, Collage of Agriculture Science and Natural Resource, Gonbad Kavous University, Gonbad Kavous, Iran.
4 Instructor, Department of Basic Sciences and Technology Engineering, University of Gonbad Kavous, Gonbad Kavos, Gonbad Kavous, Iran.
چکیده [English]

Genetic diversity is of great importance for breeding programs. In order to study the genetic diversity of 102 different rice genotypes based on seedling traits and molecular experiments, an experiment was conducted in a Completely Randomized Design with three replications. The studied traits including Plumule length, radicle length, length of the largest leaf, width of the largest leaf, number of root, fresh shoot weight, fresh weight of root and root volume, and dry weight of shoot, dry weight of root were measured. The average of the Polymorphic of information content (PIC) was estimated to be 0.2716, indicating RM1029 with the of least 0.175 and the RM216 with the highest of 0.435 (PIC). The results of Association analysis between microsatellite marker and seeding traits at normal condition indicated that the RM60B allele for root length, RM127A allele for root, RM231G allele for the fresh weight of the stem explained high percentage of variations. In saline condition, RM129H allele for stem length trait, RM12091 B allele for root attribute, RM263G allele for stem fresh weight, RM127C allele for width of largest leaf explained high percentage of phenotypic variations and were identified as important markers. The results of this experiment can be used in breeding programs.

کلیدواژه‌ها [English]

  • rice
  • Salinity
  • Marker
  • Association Analysis

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