بررسی همخوانی گروه‌بندی ژنوتیپ‌های برنج آپلند و لولند با استفاده از نشانگرهای ریزماهواره و شاخص‌های تحمل به تنش خشکی

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشیار گروه زراعت و اصلاح نباتات دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی، اردبیل

2 دانش آموخته کارشناسی ارشد گروه زراعت و اصلاح نباتات دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی

3 دانشبار ژنتیک و به نژادی گیاهی دانشکده کشاورزی دانشگاه گیلان، رشت

4 دانشیار گروه زراعت و اصلاح نباتات دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی، اردبیل، ایران.

5 دانشجوی دکتری اصلاح نباتات، دانشکده کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران

چکیده

یکی از روش‌هایی که می‌تواند میزان اعتبار نشانگرهای شناسایی شده را ارزیابی کند، بررسی هم‌خوانی گروه بندی افراد برپایه نشانگر‌های مولکولی و داده‌های فنوتیپی به‌دست‌آمده از آزمایش در شرایط عادی و تنش خشکی است. این پژوهش به منظور بررسی وجود ارتباط احتمالی بین نشانگر‌ SSR و شاخص‌های تحمل به تنش خشکی در ژنوتیپ‌های مورد نظر و همچنین گروه‌بندی ژنوتیپ‌ها بر اساس این نشانگر SSR و شاخص‌های تحمل به تنش خشکی با 40 ژنوتیپ برنج در دو محیط تنش و بدون تنش در قالب طرح بلوک‌های کامل تصادفی با سه تکرار به همراه 26 نشانگر ریزماهواره مرتبط با تحمل به تنش خشکی اجرا شد. در مجموع، 128 آلل چندشکل با میانگین 92/4 آلل به ازای هر جایگاه نشانگری تکثیر شد. بالاترین میزان PIC مربوط به نشانگر RM5672 (829/0) و کم‌‌ترین آن مربوط به نشانگر RM523(047/0) بود. تحلیل همبستگی بین عملکرد و شاخص‌های تحمل به تنش در دو شرایط تنش و بدون تنش، شاخص‌های تحمل به تنش (STI)، میانگین هندسی بهره‌وری (GMP)، شاخص میانگین عملکرد (MP) و شاخص عملکرد (YI) را به عنوان شاخص برتر در شناسایی ژنوتیپ‌های متحمل و حساس معرفی کرد. تجزیه خوشه‌ای ژنوتیپ‌های برنج مورد مطالعه به روش Ward براساس شاخص‌های تحمل به خشکی، آن‌ها را به سه گروه متحمل نیمه متحمل و حساس تقسیم کرد. با توجه به‌ این‌که ژنوتیپ‌های گروه دوم از لحاظ شاخص‌های فوق، دارای ارزش بالاتر از میانگین کل بودند، به عنوان ژنوتیپ‌های متحمل معرفی شدند که اغلب شامل ژنوتیپ‌های آپلند و یک ژنوتیپ هاشمی بودند. گروه اول نیمه متحمل و گروه سوم حساس شناخته شدند. تجزیه خوشه ای برپایه نشانگر‌های ریزماهواره نیز ژنوتیپ‌ها را به دو گروه تقسیم کرد. مقایسه این دو نوع گروه‌بندی بیانگر همخوانی شایان توجهی بین آن‌ها بود، طوری که در هر دو گروه‌بندی ژنوتیپ هاشمی قرابت نزدیکی با ژنوتیپ‌های آپلند نشان داد و همراه آن‌ها در یک گروه قرار گرفت.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Consistency of upland and Lowland rice genotypes grouping by microsatellite markers and drought tolerance indices

نویسندگان [English]

  • Omid Sofalian 1
  • Fatemeh Ajri 2
  • Atefeh Sabouri 3
  • Ali Asghari 4
  • Samira Hasanian 5
1 Assistant professor of Plant Breeding of Department of Agronomy & Plant Breeding, Faculty of Agricultural and Natural Resources, University of Mohaghegh Ardabili, Ardabil
2 MSc student of Plant Breeding of Department of Agronomy & Plant Breeding, Faculty of Agricultural and Natural Resources, University of Mohaghegh Ardabili, Ardabil.
3 Associate professor Genetic and Plant Breeding of Faculty of Agricultural Sciences, University of Guilan, Rasht
4 Associate professor of Plant Breeding of Department of Agronomy & Plant Breeding, Faculty of Agricultural and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
5 Ph.D Student of Plant Breeding, Department of Agronomy and Plant Breeding Dept., Faculty of Agricultural Sciences, University of Mohaghegh Ardabili, Ardabil 179
چکیده [English]

One way to assessing the validity of recognized markers is studing the consistency of case grouping based on molecular markers and phenotypic data obtained from the normal and drought stress conditions. In this study in order to assessing probable relationship between SSR molecular markers and drought tolerance indices in studding genotypes and grouping these genotypes based on SSR molecular markers and tolerance indices, 40 rice genotypes was used based on randomized complete block design with three replications in both normal and stress conditions. In addition, 26 microsattelite markers in relation with drought tolerance were used. Our results showed that 128 polymorphic alleles with 4.92 mean allele for each marker locus were amplified. The highest PIC value related to RM5672 (0.829) and the least related to RM523 (0.047). The corelation analysis between yield and tolerance indices in both two conditions confirmed that four indices; mean productivity (MP), geometric mean productivity (GMP), stress tolerance index (STI) and yield index (YI) were the best indices for sensitive and tolerant genotype discrimination. Grouping of genotypes based on cluster analysis using WARD method divided all of studding genotypes into three groups including tolerant, semi tolerant and sensitive. Considering the higher values of second group than the mean of the above indicators, they were introduced as tolerant genotypes, which often included upland genotypes and a Hashemi genotype. Based on our results thre firs group represent semi tolerant genotypes ant the third group represents sensitive genotypes. The cluster analysis based on microsatellite markers also divided genotypes into two groups. Comparison of these two types of grouping showed a significant correlation between them, so that in both groups the Hashemi genotype showed close proximity to the upland genotypes and was associated with them in one group.

کلیدواژه‌ها [English]

  • Cluster analysis
  • rice
  • principle component analysis
  • water deficient
  • SSR marker

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