بررسی بیوانفورماتیکی microRNA های دخیل در تنش سرما و اثر آن‌ها بر پروتئین های مرتبط با متابولیسم اسیدهای چرب در کلزا (Brassica napus L.)

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی دکتری بیوتکنولوژی گیاهی، پردیس دانشگاهی، دانشگاه گیلان، رشت، ایران

2 استاد گروه بیوتکنولوژی، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران

3 استادیار ، گروه علوم دام و طیور، پردیس ابوریحان دانشگاه تهران، ایران

4 استادیار، گروه بیوتکنولوژی، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران

چکیده

کلزا (Brassica napus) از مهم‌ترین گیاهان روغنی در سطح جهان است که علاوه بر دارا بودن ذخایر غنی اسیدهای چرب، حاوی پروتئین نیز می‌باشد. تنش سرما از عوامل محدودکننده رشد این گیاه است که می‌تواند منجر به تغییرات وسیعی به لحاظ مولکولی در درون گیاه شود. در پژوهش حاضر، به منظور بررسی ارتباط بین مولکول های miRNA، تنش سرما، اسید‌های چرب و لیپیدها، مولکول‌های miRNA با اسامی bna-miR164a، bra-miR5712، bra-miR5717، bna-miR6029، bna-miR6035، bna-N_miR2، bna-N_miR16 که قبلاً به‌صورت جداگانه در سرما و بیوسنتز اسید‌های چرب بررسی شده بودند، انتخاب شدند. سپس پیشبینی بیوانفورماتیکی ژن‌های هدف، بررسی هستیشناسی و مسیر عملکردی آن‌ها و ترسیم شبکه برهم‌کنش پروتئینها انجام شد. در نتیجه مشخص شد مولکول های miRNA که در تنش سرما مؤثرند، علاوه بر اثر تنظیمی بر ژن‌های هدفی همچون SCRM2, HOS1, STZ GLP4, PP2CA که در پاسخ به سرما و پاسخ به محرک دمایی تأثیر گذارند، بر ژن‌های هدف دیگری همچون ,KAS2 ,FAD8 ,FAD4 ,AAD3 FAD7,FAD2 که در مسیر فرآیندهای بیولوژیکی مانند بیوسنتز اسید چرب اشباع‌نشده، متابولیسم اسید‌ چرب غیر‌اشباع، بیوسنتز اسید ‌چرب، متابولیسم اسید چرب، کاتابولیسم لیپید سلولی و متابولیسم لیپید، بیوسنتز لیپید، متابولیسم لیپید سلولی نقش دارند نیز اثر گذارند. بررسی برهمکنش‌های پروتئین-پروتئین نیز حاکی از ارتباط ژن‌های هدف درگیر در تنش سرما با ژن‌های هدف درگیر در بیوسنتز اسید‌های چرب بود. گام بعدی در پژوهش‌های پیش رو بررسی بیان این مولکول‌های miRNA و ژن های هدف درطی تنش سرما است تا وجود ارتباط تنظیمی میان آن‌ها توسط روش‌های آزمایشگاهی مورد تأیید بیش‌تر قرار گیرد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Bioinformatics analysis of microRNAs related to cold Stress and their effects on proteins associated with fatty acids metabolism in rapeseed (Brassica napus L.)

نویسندگان [English]

  • Mohammad Mahdi Taghvaei 1
  • Habibollah Habibollah Samizadeh Lahiji 2
  • Mohammad Reza Bakhtiarizadeh 3
  • Mohammad Mohsenzadeh Golafazani 4
1 Ph.D. Candidate of Plant Biotechnology, Faculty of Agriculture, University Campus 2 - University of Guilan, Rasht, Iran.
2 Professor, Department of Plant Biotechnology, Faculty of Agriculture, University of Guilan, Rasht, Iran
3 Assistant Professor, Department of Animal and Poultry Sciences, Aburaihan Campus, University of Tehran, Iran
4 Assistant Professor, Department of Plant Biotechnology, Faculty of Agriculture, University of Guilan, Rasht, Iran
چکیده [English]

Rapeseed (Brassica napus) is one of the most important oil plants in the world. Not only it has rich sources of fatty acids, but also contains protein. Cold stress is one of the growth limiting factors for this plant, which causes massive molecular changes inside the plant. In this research miRNAs, bna-miR164a, bra-miR5712, bra-miR5717, bna-miR6029, bna-miR6035, bna-N_miR2, bna-N_miR16 that previously had been studied separately in cold and fatty acid biosynthesis, were selected for investigation of the relationship between microRNAs, Cold stress, Fatty Acids and Lipids. Afterwards, bioinformatics prediction of target genes, investigation of gene ontology and their functional pathway and mapping of protein interactions network were performed. As a result, it was discovered that miRNAs which are effective in cold stress, beside regulation of target genes such as SCRM2, HOS1, STZ, GLP4, PP2CA, which are responsible for cold response and thermal stimulus-response, also have regulatory roles on other target genes such as KAS2, FAD8, FAD4, AAD3, FAD7, FAD2 which are involved in biological processes such as unsaturated fatty acid biosynthesis, unsaturated fatty acid metabolism, fatty acid biosynthesis, fatty acid metabolism, cellular lipid catabolism and lipid metabolism, lipid biosynthesis, cellular lipid metabolism. Evaluation of Protein-Protein Interactions revealed that there was a relation between the target genes involved in fatty acid biosynthesis and the target genes involved in cold stress. The next step for subsequent research is to investigate the expression of these miRNAs and their target genes during cold stress and further experimental verifications to prove the regulatory relationship between them.

کلیدواژه‌ها [English]

  • Gene Ontology
  • KEGG
  • Protein-protein interaction
  • STRING

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