مطالعه خانواده ژنی ساکارز سینتاز (SUS) گندم (Triticum aestivum L.) بر اساس روش‌های بیوانفورماتیکی

نوع مقاله : علمی پژوهشی

نویسنده

گروه زراعت، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان، ایران.

چکیده

در گیاهان عالی، ساکارز سینتاز (SuS, EC 2.4.1.13) به‌عنوان آنزیم کلیدی در متابولیسم ساکارز شناخته می‌شود. این آنزیم صورت برگشت پذیر و در حضور یوریدین دی‌فسفات (UDP)، ساکارز را به فروکتوز و UDP-گلوکز تبدیل می‌کند. در این مطالعه 22 ژن ساکارز سینتاز با استفاده از روش‌های بیوانفورماتیکی در ژنوم گندم شناسایی شد که براساس جایگاه کروموزومی از TaSUS1 تا TaSUS22 نامگذاری شدند. آنالیز فیلوژنتیکی نشان داد که ژن‌های ساکارز سینتاز گندم و سایر گیاهان از نظر تکاملی به سه گروه SUSI، SUSII و SUSIII تقسیم می‌شوند. ژن‌های TaSUS هر گروه از نظر ویژگی‌های ساختاری مانند تعداد، طول و توزیع اگزون‌ها حفاظت شده هستند. ناحیه بالادست ژن‌های TaSUS دارای فراوانی متفاوتی از عناصر تنظیمی cis است که دلیلی بر پیچیدگی تنظیم بیان این ژن‌ها در مراحل نموی و یا پاسخ به تنش‌ها می‌باشد. همچنین 21 ژن TaSUS هدف miRNA مربوط به 16 خانواده متفاوت قرار می‌گیرند که اهمیت تظیم بیان ژن پس از رونویسی در این خانواده را نشان می‌هد. پروفایل بیانی مکانی/زمانی این ژن‌ها مشخص کرد که ژن‌های TaSUS مربوط به زیرگروه SUSI نقش مهمی در رشد و نمو گندم بر عهده دارند. این نتایج نگاه جدیدی به تکامل ژن‌های TaSUS بوده و اطلاعات پایه از نظر تکاملی و کارکردی احتمالی، برای انتخاب و ارزیابی نقش این ژن‌ها در متابولیسم ساکارز در شرایط رشدی مختلف در گندم را فراهم می‌کند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Analysis of the sucrose synthase (SUS) gene family in wheat (Triticum aestivum L.) based on bioinformatics methods

نویسنده [English]

  • Naser Mohammadian Roshan
Department of Agriculture, Lahijan Branch Islamic Azad University, Lahijan, Iran.
چکیده [English]

In higher plants, sucrose synthase (SuS, EC 2.4.1.13) is widely considered as a key enzyme involved in sucrose metabolism and catalyzes the reversible cleavage of sucrose into fructose and UDP- glucose. Here, 22 SuS genes have been identified in the genome of wheat by using bioinformatics methods and renamed TaSUS1 to TaSUS22 according to their chromosomal locations. Phylogenetic analysis revealed that the sucrose synthase genes in wheat and other plants were divided into three evolutionary groups, named SUS I, SUS II and SUS III, respectively. SuS genes in the same group showed similar structural characteristics, such as exon number, size and length distribution. The upstream regions of TaSuS genes had variable frequencies of cis-regulatory elements that could show regulation at different developmental stages and/or differential regulation in response to stress, while 21 genes had targets for 16 different miRNA families that show the importance of posttranscriptional regulation for TaSUS genes. Temporal and spatial expression profiling indicates a potential role for SUSI genes during growth and development in wheat. These results give new insights into the evolution and basic information that will aid in elucidating the functions of the wheat SuS gene family.

کلیدواژه‌ها [English]

  • Gene Expression
  • Database
  • Evolution
  • Phylogeny Tree
  • Metabolism

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