شناسایی ژن های مرتبط با خصوصیت نیمه سازگاری بین سویه‌های Rizobium leguminosarum و لوتوس بورتیایی با روش GWAS

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی دکتری دانشجوی رشته اصلاح‌نباتات گرایش ژنتیک مولکولی دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران.

2 دانشیار، گروه اصلاح نباتات و بیوتکنولوژی دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران.

چکیده

همزیستی بین گیاه و باکتری با تشکیل گره و تثبیت نیتروژن بر کمیت محصول و همچنین محتوای پروتئین گیاه دارای اهمیت بسیار زیادی می‌باشد. در این پژوهش جهت شناسایی ژن‌های باکتریایی مرتبط با تولید گره، تعداد 194 سویه باکتری Rizobium leguminosarum نیمه همزیست با گیاه لوتوس بورتیایی مورد استفاده قرار گرفت. صفات مرتبط با گره‌زایی (تعداد گره سفید، تعداد گره قرمز و تعداد کل گره) در 7، 14، 21 و 28 روز بعد از تلقیح اندازه‌گیری شد. نتایج حاصل از تجزیه به مولفه‌های اصلی، حاکی از تفکیک کامل بین سویه‌ها با ژنوم‌هایA ، C و D بود. علاوه بر آن، نتایج مطالعه سرتاسری ژنوم (GWAS) نشان داد که اپرون با فعالیت فاکتور ترشحی در القای مقاومت بر صفت گره قرمز موثر می‌باشد.

یاد آور می شود که به دلیل برخورد بسیار مختصر با چکیده مقاله این چکیده مقاله از تعداد دویست کلمه مجاز در این قسمت بسیار بسیار کمتر شده است که به این وسیله برای رسانده به تعداد دویست کلمه در این قسمت این توضیحات را نوشته ام که بتوانم مجوز ورود به مرحله بعد را دریافت نمایم. از این که مجبور به ارائه توضیح در این قسمت شدم عذرخواه هستم و لذا شایسته است عذر نویسنده مسئول را با بزرگواری خود بپذیرید.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Identification of genes related to the semi-compatibility characteristic between Rizobium leguminosarum strains and Lotus burttii by GWAS method

نویسندگان [English]

  • Mohammad Zarrabian 1
  • Gholam Ranjbar 2
  • Seyed Kamal Kazemitabar 2
  • Hamid Najafi Zarrini 2
1 Ph.D. Candidate in Molecular Genetics, Faculty of Crop Sciences, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran.
2 Associate Professor, Department of Plant Breeding and Biotechnology, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran.
چکیده [English]

The co-existence of plant and bacteria with nodule formation and nitrogen fixation has a great effect on plant production, as well as plant protein content. In this study, 194 strains of semi-symbiotic Rizobium leguminosarum were used to identify bacterial genes related to nodule formation on L. burttii. Nodule related traits (number of white nodes, number of pink nodes, and total number of nodes) were measured at 7, 14, 21 and 28 days after inoculation. Principal Component Analysis (PCA) showed complete separation between strains with genomes A, C and D. In addition, the results of genome-wide association study (GWAS) showed that operon with secretory factor activity is effective in inducing resistance to red node trait.

Doing such a job is very hard to understand an I prefer to have nothing for reaching to two hundred words until I can have green light for transferring to the next step of sending my manuscript. Sorry for that.

کلیدواژه‌ها [English]

  • Lotus
  • GWAS
  • Rizobium leguminosarum
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