شناسایی خانواده ژنی ERF در گیاه شورزیست Aeluropus littoralis و بررسی الگوی بیان آن‌ها در پاسخ به تنش شوری

نوع مقاله : علمی پژوهشی

نویسندگان

1 استادیار، گروه مهندسی ژنتیک و بیولوژی، پژوهشکده ژنتیک و زیست‌فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران.

2 دانشجوی دکتری اصلاح نباتات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران.

چکیده

تنش شوری یکی از تنش‌های غیرزیستی محدودکننده رشد و نمو گیاهان می‌باشد. عامل پاسخ‌دهنده به اتیلن (ERF) خانواده ای از عوامل رونویسی دخیل در رشد و نمو گیاهان و نیز پاسخ‌دهنده به تنش‌های زیستی و غیرزیستی بوده و با توجه به اهمیت این خانواده در پاسخ به تنش شوری، در این تحقیق شناسایی ژن‌های این خانواده در گیاه شورزیست چمن شور آلوروپوس لیتورالیس (Aeluropus littoralis) مدنظر قرار گرفت. در مجموع، 36 ژن غیر تکراری ERF در ژنوم آلوروپوس لیتورالیس شناسایی شد. ترسیم درخت فیلوژنتیک بر مبنای همولوژی با گیاه آرابیدوپسیس، خانواده ژنی AlERF را به شش گروه مشخص (B1 تا B6) طبقه‌بندی نمود. تجزیه و تحلیل ساختار ژنی نشان داد که تعداد اینترون‌ در ژن‌های AlERF بین صفر تا دو متغیر بود. تجزیه و تحلیل ‌موتیف‌های حفاظت‌شده و جستجوی دمین در توالی‌های پروتئینی AlERF نشان داد از ده موتیف شناسایی شده، تنها موتیف‌های یک و دو در ساختار دمین AP2/ERF مشارکت دارند. بر اساس داده‌های ترانسکریپتوم و نمودار Heatmap، ژن‌ AlERF6.3 ببیشترین بیان را در شرایط تنش شوری در بافت ریشه نشان داد و بیشترین کاهش بیان نیز در ژن‌ AlERF6.7 در شرایط بازیابی در بافت برگی مشاهده شد. این الگوی متفاوت بیان ژن‌ها در بافت‌های برگ و ریشه در مواجهه با تنش شوری، حاکی از وجود مکانیسم‌های تنظیمی متفاوت در بیان این ژن‌ها می‌باشد. نتایج این مطالعه به‌عنوان اولین گزارش در مورد خانواده ژنی ERF در آلوروپوس لیتورالیس، اطلاعات پایه‌ای را برای مطالعات بیشتر در مورد خصوصیات کارکردی ژن‌های AlERF فراهم می‌نماید.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Identification of the ERF gene family in Aeluropus littoralis halophyte plant and analysis of their expression pattern in response to salt stress

نویسندگان [English]

  • Seyyed Hamidreza Hashemi-petroudi 1
  • Samira Mohammadi 2
1 Assistant Preofessor, Department of Genetic Engineering and Biology, Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran.
2 Ph.D. Candidate in Plant Breeding, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran.
چکیده [English]

Salt stress is one of the abiotic stresses limiting plant growth and development. The ethylene response factor (ERF) is one of the transcription factor family that involved in plant development and responses to biotic and abiotic stresses. Regarding to importance role of genes belonging to ERF gene family in plant responses to salt stress, identification of these genes in the Aeluropus littoralis, halophyte plant, was considered in this study. In total, 36 non-redundant ERF genes were identified in A. littoralis genome. The phylogenetic tree classified the AlERF gene family into six distinct groups (B1 to B6) based on hemology with the Araboidopsis thaliana. Gene structure analysis revealed that AlERF genes contained zero to two introns. Domain search and conserved motif analyses in AlERF protein sequences determined that 2 motifs (1 and 2) out of the identified 10 motifs participate in the AP2/ERF domain structure. Based on transcriptome data and heatmap diagram, AlERF6.3 gene was expressed more in root tissue under salinity stress, and the least expression level was observed in AlERF6.7 gene in leaf tissue under recovery conditions. The different expression patterns of genes in leaf and root tissues under salt stress suggested different regulatory mechanisms in the gene expression. The results of this study, as the first report on the ERF gene family in A. littoralis, provides basic information for further studies of the functional characteristics of AlERF genes.

کلیدواژه‌ها [English]

  • Aeluropus littoralis
  • ERF transcription factor
  • Gene family
  • Phylogenetic relationships
  • salt stress

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