اعتبارسنجی مولکولی ژن‌های پاسخگو به تنش شوری و ارزیابی تنوع آللی آنها در موتانت‌های برنج

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی دکتری اصلاح نباتات دانشگاه علوم کشاورزی و منابع طبیعی ساری، پژوهشکده ژنتیک و زیست‌فناوری کشاورزی طبرستان، ساری، ایران.

2 استاد گروه اصلاح نباتات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، پژوهشکده ژنتیک و زیست‌فناوری کشاورزی طبرستان، ساری، ایران

3 دانشیار گروه اصلاح نباتات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

4 گروه مهندسی ژنتیک و بیولوژی، پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

چکیده

انتخاب به کمک نشانگر (MAS) نوعی گزینش بوده که تحت تأثیر محیط نیست. موفقیت برنامه‌های به‌نژادی بر پایه MAS به انتخاب و اعتبارسنجی آغازگرهای مورد استفاده بستگی دارد. در این تحقیق، جهت اعتبارسنجی ژن (های) مرتبط با تنش شوری و ارزیابی تنوع آللی این آغازگرها در لاین‌های موتانت برنج، الگوی باندی 18 نشانگر SSR، بر روی نمونه برگی 14 لاین موتانت (M9) برنج، به همراه 2 شاهد حساس (IR29 و سپیدرود) و 2 شاهد متحمل (Nonabokra و دیلمانی) در سال 1398 در پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان بررسی شد. 11 آغازگر بر مبنای تحلیل الگوی باندی در ارقام حساس/ متحمل انتخاب گردیدند. آنالیز مولکولی داده‌ها نشان داد که بیشترین محتوای اطلاعات چندشکلی(PIC) مربوط به آغازگرهای OsMAPK4، OsCML11 وOsCPK17 به‌ترتیب به میزان 46/0، 46/0 و 38/0 بود. بالاترین شاخص نشانگر(MI) مربوط به دو آغازگر OsMAPK4 و OsCML11، به میزان 23/0 بود. آغازگر OsCAX (D) دارای کمترین PIC و MI به‌ترتیب به میزان 05/0 و 11/0 بود. لاین‌های موتانت مورد مطالعه توسط تجزیه کلاستر و بای پلات به ترتیب به 3 و 4 گروه تقسیم شدند. سه آغازگر OsCML11، OsMAPK4 وOsCPK17 به‌ترتیب بر روی کروموزوم‌های 1، 6 و 7 به‌عنوان کاراترین آغازگر‌ها در شناسایی میزان تنوع ژنتیکی بین ژنوتیپ‌های برنج مورد ارزیابی در این مطالعه شناسایی شدند. نظر به اینکه آغازگرهای نامبرده پیوستگی بسیار بالایی با ژن‌های مقاومت به شوری دارند می‌توان پیش‌بینی کرد که لاین‌های G1 (M9-P1-7-2-1)، G8 (M9-P3-21-1-1) و G9

(M9- P6-7-1-1) تحمل بالایی به تنش شوری داشته باشند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Molecular validation of genes responsive to salinity stress and evaluation of their allelic diversity in mutant rice

نویسندگان [English]

  • Morteza Oladi Ghadicolaei 1
  • Ghorban Ali Nematzadeh 2
  • Ali Ranjbar 3
  • Hamidreza Hashemi 4
1 Ph. D. Student of Plant Breeding, Sari Agricultural Sciences and Natural Resources University (SANRU), Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Iran.
2 Professors, Department of Plant Breeding, Sari Agricultural Sciences and Natural Resources University (SANRU), Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari., Iran.
3 Associated Professor, Department of Plant Breeding, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran.
4 4. Department of Genetic Engineering and Biology, Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran.
چکیده [English]

Marker-assisted selection (MAS), a selective method which is not influenced by environmental factors. The success of MAS-based breeding programs depends on the selection and validation of the markers used. In this study, to validate the gene(s) associated with salinity stress and evaluation of allelic diversity of these markers in mutant rice lines, Band pattern of 18 SSR markers on a leaf sample of 14 mutant lines (M9) of rice, along with 2 susceptible controls (IR29 and Sepidrood) and 2 tolerant controls (Nonabokra and Dylmani) in 1398 in Genetics & Agricultural Biotechnology Institute of Tabarestan (GABIT). 11 primers were selected based on band pattern analysis in susceptible / tolerant cultivars. The molecular analysis results showed that OsMAPK4, OsCML11 and OsCPK17 had highest polymorphic information content (PIC). OsMAPK4 and OsCML11 had highest marker index (MI) at a rate of 0.23. The lowest PIC (0.05) and MI (0. 11) was accounted for OsCAX (D). Cluster analysis of molecular data, divided rice genotypes into three distinct groups. However, analysis of Biplot classified the genotypes into four different groups. In this study, 3 genes OsCML11, OsMAPK4 and OsCPK17 were identified on chromosomes 1, 6 and 7 respectively, as the most efficient primers in identifying the genetic diversity between the rice genotypes, considering that these primers have a very high linkage with salinity resistance genes, can be predicted that 3 lines G1 (M9-P1-7-2-1), G8 (M9-P3-21-1-1) and G9 (M9-P6 -7-1-1) have high tolerance to salinity stress.

کلیدواژه‌ها [English]

  • Rice
  • mutants
  • salinity stress
  • MAS

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