تعیین خصوصیات و نقش خانواده پروتئین‌های دهیدرین در تحمل به تنش‌های غیر زیستی در گیاه Aeluropus littoralis

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی دکتری اصلاح نباتات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران.

2 دانشیار، گروه اصلاح نباتات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران.

3 دانشیار گروه اصلاح نباتات، دانشگاه علوم کشاورزی و منابع طبیعی ساری (SANRU)

4 استادیار، گروه مهندسی ژنتیک و بیولوژی، پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران.

چکیده

دهیدرین‏ها (DHNs) متعلق به گروه دوم پروتئین‏های LEA می‌باشند که اعضای آن در اواخر دوره جنین‏زایی در بذر و در پاسخ به تنش‏های غیرزیستی مانند شوری، خشکی و سرما در بافت رویشی تجمع می‏یابند. این پروتئین‌ها بر اساس توالی و تعداد قطعات K (لیزین)، S (سرین) و Y (تیروزین) به پنج زیرگروه SKn، YnSKn، KnS، Kn و YnKn طبقه‌بندی می‏شوند. در این مطالعه، 5 ژن کدکننده پروتئین دهیدرین (DHN) در ژنوم آلوروپوس لیتورالیس (Aeluropus littoralis) به‌عنوان یک گیاه هالوفیت متعلق به خانواده گندمیان شناسایی و خصوصیات فیزیکوشیمیایی، جایگاه سلولی، موتیف‌های حفاظت‌شده و ساختار ژنی آن‌ها تعیین و روابط تکاملی بین این پروتئین‌ها در سایر گونه‌ها نیز مدنظر قرار گرفت. پروتئین‌های AlDHN بر اساس دامنه‌های بسیار حفاظت‌شده K، S و Y در زیرگروه YnSKn قرار گرفتند. الگوی بیان ژن AlDHN.5 به‌عنوان همولوگ ژن RAB18 ارابیدوپسیس (AT5G66400) در دو بافت برگ و ریشه تحت تنش‌های شوری، خشکی، سرما و تیمار آبسیزیک اسید، مورد بررسی قرار گرفت. آنالیز الگوی بیان این ژن در دو بافت برگ و ریشه نشان داد که این ژن در تنش‏های شوری، خشکی، سرما و هورمون ABA در بافت برگ در مقایسه با ریشه بیشتر بیان می‏شود. این نتایج، نشان‌دهنده نقش مهم دهیدرین‏ها، در پاسخ به تنش‏های غیر زیستی می‌باشد. این مطالعه پایه و اساس مطالعات بیشتر در مورد تنظیم بیان این ژن‏ها در شرایط مختلف محیطی می‌باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Characterization and Role of the Dehydrin Proteins Family in Abiotic Stress Tolerance in Aeluropus littoralis

نویسندگان [English]

  • Behnaz Dolatabadi 1
  • Gholam Ali Ranjbar 2
  • Hamid Najafi-Zarrini 3
  • Seyyed Hamidreza Hashemi-Petroudi 4
1 Ph.D. Candidate in Plant Breeding, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran.
2 Associate Professor, Department of Plant Breeding, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran.
3 Associate professor of Department of Plant Breeding, Sari Agricultural Sciences and Natural Resources University (SANRU)
4 Assistant Professor, Department of Genetic Engineering and Biology, Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran.
چکیده [English]

Dehydrins (DNHs) belong to group II of LEA (Late Embryogenesis Abundant) protein family which are expressed in late embryogenesis and accumulate in vegetative tissues in response to multiple abiotic stresses such as salt, drought and cold stress These proteins could be classified to five subgroups (YnSKn, Kn, SKn, KnS, and YnKn) based on the sequence and number of K, S, and Y segments. In this study, 5 genes encoding dehydrin protein (DHN) were identified in Aeluropus littoralis, genome as a halophyte grass, belonging to the Poaceae family and physicochemical characteristics, cell localization, conserved motifs and gene structure were determined and evolutionary relationships among different species were considered. AlDHN proteins were classified in the YnSKn subgroup based on highly conserved domains. The expression pattern of AlDHN.5 gene as a homologue of RAB18 (AT5G66400) gene was examined in both leaf and root tissues under salinity, drought, cold stresses and abscisic acid treatment. Analysis of the expression pattern of this gene in both leaf and root tissues showed that this gene is more expressed in leaf tissue compared to root under drought, cold stresses and ABA treatment. Current study lays the foundation for further studies into the regulation of their expression under various environmental conditions.

کلیدواژه‌ها [English]

  • RT-qPCR analysis
  • YnSKn subgroup
  • Gene structure
  • Motif
  • Halophyte

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