تأثیر سویه‌های مختلف Agrobacterium rhizogenes، نوع و سن ریزنمونه بر تولید ریشه‌های مویین در گیاه سنبل‌الطیب (Valeriana officinalis L.)

نوع مقاله : علمی پژوهشی

نویسندگان

1 استادیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران.

2 کارشناسی ارشد، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران.

3 دانشیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران و دانشیار، پژوهشی مرکز پژوهش‌های مجلس شورای اسلامی، تهران، ایران.

چکیده

گیاه سنبل‌الطیب (Valeriana officinalis L.) از خانواده Valerianaceae به‌عنوان گیاه دارویی‌ ارزشمند همواره مورد توجه بوده است که از آن برای درمان بیماری‌های عصبی استفاده می‌شود. پژوهش به‌صورت آزمایش فاکتوریل در قالب طرح کاملاً تصادفی شامل سویه‌های باکتری (R1000, LBA9402, C58, A4, GM, ATTCC15834)، سن ریزنمونه جهت تلقیح (10، 20 و 30‌ روز) و ریزنمونه‌های مختلف گیاه سنبل‌الطیب (برگ و ریشه) در سه تکرار انجام گرفت. جهت کشت باکتری‌ها از محیط کشت MYA جامد و جهت تهیه سوسپانسیون باکتری به‌منظور تلقیح از محیط MYA مایع حاوی آنتی‌بیوتیک ریفامپسین با غلظت 50 میلی‌گرم در لیتر استفاده گردید. دو روش تلقیح (روش غوطه‌وری و تزریق) بر درصد ریشه‌زایی ریز‌نمونه‌ها مورد ارزیابی قرار گرفت و روش تزریق در القای ریشه مویین بر ریزنمونه‌ها مؤثر واقع نشد. پس از مشخص شدن بهترین سویه و بهترین ریزنمونه، جهت پرآوری ریشه‌های مویین کلون‌ها در محیط کشت جامد MS ،1/2 MS و MS + 0.2 mg/L NAA کشت گردیدند، مشاهده شد که ریشه‌های قرار داده شده در محیط کشت MS بیشترین زیست‌توده را داشتند. سویه ATCC15834و A4 رشد یافته در محیط MYA روی ریز نمونه برگی 30 روزه مناسب‌ترین سویه‌ها بودند. بیشترین طول ریشه‌ و تعداد ریشه ‌مویین در القای با سویه A4 دیده شد. ریشه‌های مویین القاء شده در محیط کشت MS حاوی 3 درصد ساکارز در دمای 25 درجه سانتی‌گراد بیشترین میزان رشد ریشه‌های مویین را نشان داد. نتایج نشان داد که کاربرد سویه‌های ATTCC15834, R1000, LBA9402 و A4 باعث القای ریشه‌های مویین در گیاه سنبل‌الطیب شد و دو سویه دیگر در القای ریشه‌های مویین ناکارآمد بودند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of different strains of Agrobacterium rhizogenes, type and age of explant on hairy root production in Valeriana officinalis L.

نویسندگان [English]

  • Mohammad Ali Aazami 1
  • Samieh Ghafury 2
  • Mohammad Bagher Hassanpouraghdam 3
1 Assistant Professor, Department of Horticulture, Faculty of Agriculture, University of Maragheh, Maragheh, Iran.
2 M.Sc. Student, Department of Horticulture, Faculty of Agriculture, University of Maragheh, Maragheh, Iran.
3 Associate Professor, Department of Horticulture, Faculty of Agriculture, University of Maragheh, Maragheh, Iran and Research Associate Professor, Islamic Parliament Research Center, Tehran, Iran.
چکیده [English]

Valeriana officinalis from the valerianaceae is a precious medicinal plant has been employed for the treatment of mental ailments. The study was a factorial experiment in a CRD including bacterial strains (R1000, LBA9402, C58, A4, GM, and ATTCC15834), age of explants for inoculation (10, 20 and 30 days) and different explants of valerian (leaves and roots) in three replications. Solid Manitol Yest exteract Agar (MYA) medium was used for bacterial culture and MYA medium containing rifampicin antibiotic (50 mg/l) was used to prepare the bacterial suspension for inoculation. Two inoculation methods (floating and injection method) were evaluated on the percentage of rooting and the injection method was not effective in hairy root induction on explants. After determining the best strain and the best explants of the clones were cultured in MS, 1/2 MS and MS+0.2 mg/L NAA solid culture medium it was observed that the roots placed in MD medium were the most they had biomass. Strains of ATCC15834 and A4 on the MYA medium on 30 days old leaf explants was the best strain than others. The optimized root length and the top hairy root number were induced by A4 strain. The newly produced hairy root showed the highest growth on MS medium enriched with 3% sucrose at 25°C. Our results showed that the application of ATTCC15834, R1000, LBA9402 and A4 strains induced hairy roots in valerian and the other two strains were ineffective.

کلیدواژه‌ها [English]

  • Agrobacterium rhizogenes
  • Valeriana officinalis L
  • strain
  • Hairy root

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