تعیین تنوع ژنتیکی ژنوتیپ‌های نخود (Cicer arietinum L.) متحمل به تنش خشکی با استفاده از نشانگرهای SSR و CAPS

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد بیوتکنولوژی و به نژادی گیاهی، دانشکده کشاورزی دانشگاه فردوسی مشهد، مشهد ایران

2 پژوهشکده علوم گیاهی، ٰدانشگاه فردوسی مشهد، مشهد، ایران

3 گروه بیوتکنولوژی گیاهی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

نخود دومین حبوبات مهم مناطق خشک و نیمه‌خشک جهان بوده و اغلب به‌صورت دیم کشت‌شده و تنش خشکی عملکرد آن را تا 50%کاهش می‌دهد. در این پژوهش میزان تنوع ژنتیکی 9 ژنوتیپ‌ نخود منتخب متحمل به خشکی 2 رقم حساس رایج، با استفاده از 11 آغازگر SSR و 6 آغازگر CAPS تعیین و 10 آغازگرهای SSR مؤثر، دوباره در جمعیت تصادفی ژنوتیپ‌های کاندیدای متحمل به سرما بررسی شد. آنالیز باندهای SSR در آزمایش نهایی درمجموع 40 آلل تولید کرد که 37 آلل چند شکل بودند. میانگین تعداد آلل‌های چند شکل به ازای هر مکان ژنی 9/3 و در دامنه 9-2 بود. محتوای اطلاعات چند شکل (PIC) نیز بین 21/0 تا 85/0 متغیر بود. آنالیز خوشه‌ای بر اساس الگوریتم UPGMA و ضریب تشابه جاکارد، ژنوتیپ‌ها را در 9 گروه شامل چهار گروه از ژنوتیپ‌های متحمل به خشکی، دو گروه حساس و سه گروه دیگر متعلق به جمعیت تصادفی تفکیک نمود. چندشکلی‌های SSR و CAPS نشان دادند که دو ژنوتیپ MCC544 و MCC392 در گروه متحمل‌ها متمایزتر و تفاوت ژنتیکی آن‌ها با بقیه ژنوتیپ‌های متحمل به خشکی بیشتر بود، درحالی‌که MCC537 و MCC696 نیز با قرابت بالا در گروه دیگری قرار گرفتند و MCC80 حد وسط ژنوتیپ‌های متحمل قرار گرفت. MCC427 با رقم رایج کشور MCC358 در یک گروه و رقم بین‌المللی MCC252 با MCC302، در گروه‌ نسبتاً حساس به خشکی قرار گرفتند. همچنین شباهت ژنتیکی میان ژنوتیپ‌های جمعیت منتخب متحمل به خشکی و نیز متحمل به سرما وجود نداشت که می‌تواند به دلیل عدم وجود اثر پلئوتروپی ژن‌ها در این نوع تنش‌ها بوده و برای اهداف بهنژادی نخود نیز می‌تواند بااهمیت باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Determination of genetic variation of drought tolerant chickpea genotypes using SSR and CAPS markers

نویسندگان [English]

  • Soroush Saberi Mahmoodabaadi 1
  • Saeedreza Vessal 2
  • Abdolreza Bagheri 3
  • Saeed Malekzadeh Shafaroudi 3
1 M.Sc. of Agricultural Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
2 Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
3 Department of Biotechnology and Plant breeding, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده [English]

Chickpea is the second most important pulses in arid and semi-arid regions of the world. This product is often cultivated as rainfed crop, so drought stress causing 50% yield loss. In this research, 11 SSR and 6 CAPS primer pairs were used to evaluate genetic variation among 9 chickpea candidate genotypes for drought tolerant and 2 common sensitive cultivars. Ten effective SSRs were again evaluated in a population containing random genotypes for cold-tolerance. SRR band analysis in final experiment generated 40 alleles, of which 37 were polymorphic. The average number of polymorphic alleles for each gene site was 3.9 with the range of 2-9 alleles. The polymorphic information content (PIC) varied from 0.21 to 0.85. The cluster analysis, based on the UPGMA algorithm and Jaccard's similarity coefficient, splited the genotypes into nine distinct groups including four groups of drought tolerant genotypes, two relatively drought tolerant groups and other three groups belonging to the random population. The polymorphism results of the SSR and CAPS markers indicated that the two genotypes MCC544 and MCC392 were more distinctive from other drought tolerant genotypes. MCC537 and MCC696 were also highly related in a separate group, and the MCC80 was allocated in average position among tolerant genotypes. MCC427 and a common cultivar MCC358 were classified into one group as well as the international cultivar MCC252 with MCC302 genotypes. The results indicated the lack of a genetic similarity between selected population of drought tolerant candidates and cold tolerant candidates. This could indicate that there might be no pleiotropic effect among the genes involved in these stresses, which can be important for breeding purposes of this crop.

کلیدواژه‌ها [English]

  • Chickpea
  • a drought
  • SSR marker
  • CAPS marker
  • polymorphism

مراجع

Abed M, Ganjeali A, Tahery G, Zare L (2015) Examine the morphological and physiological haracteristic of those genotypes hoped to be resistant to drought in chickpea (Cicer arietinum L.) in complementary irrigation. Science J. 36(3): 573-576
Doyle JJ and Doyle J L (1990) A rapid total DNA preparation procedure for fresh plant tissue. Focus. 12: 13-15.
FAOSTAT (2018) www.fao.org. http://www.fao.org/faostat/en/#data/QC. Accessed 15 March 2020.
Ganjeali A, Bagheri A, Porsa H (2009) Evaluation of chickpea (Cicer arietinum L.) germplasm for drought resistance. Iranian J. of F. Crops Res. 7(1):183-194.
Ganjeali A, Porsa H, Bagheri A (2011a) Response of yield and morphophysiological characteristics of earliness chickpea genotypes (Cicer arietinum L.) under drought stress. Iranian J. of Pulses Res. 2(1): 65-80.
Ganjeali A, Porsa H, and Bagheri A (2011b) Assessment of Iranian chickpea (Cicer arietinum L.) germplasms for drought tolerance. Agricultural Water Management. 98(9): 1477-1484.
Ganjeali A, Rahbarian R, Bagheri A, Malekzadeh Shafaroudi S (2014) Study on drought stress effects on morphological, physiological and biochemical characteristics of chickpea genotypes (Cicer arietinum L.) under field condition. Iranian J. of Pulses Res. 5(1): 91-102.
Hussain SS (2006) Molecular breeding for abiotic stress tolerance: drought perspective. Proc. of the Pak. Academy of Sciences. 43(3): 189-210.
Hüttel B, Winter P, Weising K, Choumane W, Weigand F, and Kahl, G (1999) Sequence-tagged microsatellite site markers for chickpea (Cicer arietinum L.). Genome. 42(2): 210-217.
Jagnathan D, Thaudi M, Kale S, Azam S, Roorkiwal M, Gaur PM, Kishor PBK, Nguyen H, Sutton T, Varshney RK (2015) Genotyping-by-sequencing based intra-specific genetic map refines a ‘‘QTL-hotspot” region for drought tolerance in chickpea. Molecular Genetics and Genomics. 290: 559-571.
Junjian N, Colowit PM, Mackill DJ (2002) Evalution of genetic diversity in rice subspecies by microsatellite markers. Crop. Science. 42: 601-607
Kale S, Jagnathan D, Ruperao P, Chen C, Punna R, Kudapa H, Thaudi M, Roorkiwal M, Katta M, Doddamani D, Garg V, Kishor PB, Gaur P, Nguyen H, Batley J, Edwards D, Sutton T, Varshney Rk (2015) Prioritization of candidate genes in “QTL-hotspot” region for drought tolerance in chickpea (Cicer arietinum L.). Scientific reports. 5: 15296.
Lahoot F, Zeinolabedini M, Karimi J, Shahbazi M, Sadeghzadeh B (2016) Assessment of genetic diversity of Iranian and non-Iranian barely genotypes (Hordeum vulgare L.) using SSR markers. Crop. Biotech. 6(15): 25-35.
Mahfoozi S, Amini A, Chaichi H, Jasemi S, Nazeri M, Abedi Oskooyi M, Aminzadeh GH, Rezayi M (2009) Study on Grain Yield Stability and Adaptability of Winter Wheat Genotypes Using Different Stability Indices Under Terminal Drought Stress Conditions. Seed and Plant Improvement J. 25(1): 65-82.
Mohammadi Nezhad GH, Ghasem Khani M, Zare R, Sardouei Nasab S, Sabouri H (2013) Evaluation of allelic diversity of microsatellite markers in QTL region attributed to salinity tolerance in Iranian rice cultivars. J. of Plant Production. 20(3): 145-157.
Nezami A, Pooramir F, Momeni S, Porsa H, Ganjeali A, Bagheri A (2012) Evaluation of a subset of chickpea germplasm collection of Ferdowsi University of Mashhad Seed Bank II. Kabuli type chickpeas. Iranian J. of Pulses Res. 20(3): 145-157.
Qureshi ST, Yasmeen A, Abassi AR, Qureshi AS, Gafoor A, Memnon M, soomro N (2015) Evaluation of chickpea genotypes under different environments for stability of quantitative traits. Sindh University Res. J. (Sci. Ser.), 47(3) 559-562.
Sabouri A, Sabouri H, Dadras A (2013) Association analysis of closely linked markers to major QTLs Saltol and SKC1 and salt tolerance-related traits in rice varieties. Cereal res. 3(1): 53-68.
Saxena NP (2003) Management of Agriculture Drought “Agronomic and Genetic Options”. Science Publishers Inc, NH, USA.
Olfati JA, Samizadeh H, Peyvast Gh, Rabie B, Khodaparast SA (2010) Parental line selection for cucumber hybrid seed production by principal component analysis. International J. of Vegetable Science. 16(4), 316-325.
Petit JR, Jouzel J, Raynaud D, Barkov NI, Barnola JM, Basile I, Delaygue G (1999) Climate and atmospheric history of the past 420,000 years from the Vostok ice core, Antarctica. Nat. 399: 429-436.
Porsa H, Nezami A, Bagheri A, Najibnia S (2016) Agronomic assessment of cold tolerant chickpea (Cicer arietinum L.) genotypes in fall sowing at Mashhad conditions. Iranian J. of Pulses Res. 7(1): 37-353.
Rehman A, Malhotra R, Bett K, Tar'an B, Bueckert R, Warkentin T (2011) Mapping QTL associated with traits affecting grain yield in Chickpea (Cicer arientinum L.) under terminal drought stress. Crop Science. 51(2): 450-463.
Rehman AU (2009) Characterization and molecular mapping of drought tolerance in kabuli chickpea (Cicer arietinum L.). PhD thesis, University of Saskatchewan, Saskatoon.
Semagn K, Bjørnstad Å, Ndjiondjop M (2006) An overview of molecular marker methods for plants. African J. of Biotechnology. 5(25): 2540-2568.
Varshney RK, Thudi M, Nayak SN, Gaur PM, Kashiwagi J, Krishnamurthy L, Tripathi S (2014) Genetic dissection of drought tolerance in chickpea (Cicer arietinum L.). Theoretical and Appl. Genetics. 127(2): 445-462.
Varshney RK, Thudi M, Pandey MK, Tardieu F, Ojiewo C, Vadez V, Whitbread AM, Siddique KHM, Nguyen HT, Carberry PS, Bergvinson D (2018) Accelerating genetic gains in legumes for the development of prosperous smallholder agriculture: integrating genomics, phenotyping, systems model- ling and agronomy. J Exp Bot. 69: 3293-3312
Winter P, Pfaff T, Udupa SM, Huttel B, Sharma PC, Sahi S, Arreguin-Espinoza R, Weigand F, Muehlbauer FJ, Kahl G (1999) Characterization and mapping of sequence-tagged microsatellite sites in the chickpea (Cicer arietinum L.) genome. Molecular and Gen. Genetics. 262: 90-101.

فایل ها

هم رسانی

ارجاع به این مقاله

آمار