با همکاری مشترک دانشگاه پیام نور و انجمن بیوتکنولوژی جمهوری اسلامی ایران

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد، دانشکده کشاورزی، دانشگاه پیام نور، تهران، ایران

2 استادیار، دانشکده علوم پایه، دانشگاه پیام نور، تهران، ایران

3 استادیار، دانشکده کشاورزی، دانشگاه پیام نور، تهران، ایران

چکیده

نانوذرات به دلیل خصوصیات متمایز خود استفاده گسترده ای در علوم مختلف از جمله بیوتکنولوژی، پزشکی و غیره دارند. بیش از نیمی از سهم تولید نانوذرات در دنیا به نانوذرات نقره اختصاص یافته که عمدتا به دلیل خاصیت ضد باکتری آنها می باشد. در روش‌های مرسوم شیمیایی برای سنتز نانوذرات نقره معمولا از ترکیبات احیا کننده ای استفاده می شود که سازگار با محیط‌ زیست نبوده و گاهی سمی می باشند . توسعه روش های سنتز زیستی برای تهیه زیستی نانوذرات بسیار مهم و حیاتی به نظر می‌رسد. در این تحقیق از عصاره گیاهان اکالیپتوس (Eucalyptus obliqua ) و نعناع (Mentha spicata) به عنوان عامل‌ احیا کننده برای تولید بیولوژیک نانوذرات نقره استفاده گردید. با افزودن عصاره گیاهی به محلول نیترات نقره در دما و زمان‌های مختلف تولید نانوذرات نقره بررسی شد. تغییر رنگ از زرد‌ کمرنگ به قهوه‌ای تیره نشان دهنده تشکیل نانوذرات نقره بود. همچنین وجود پیک جذبی (SPR) قوی در طول موج بین 420 تا 460 با استفاده از دستگاه طیف‌سنجی UV/Visible نیز تأییدی بر تولید نانوذرات نقره بود. تشکیل این نانوذرات نیز توسط میکروسکوپ الکترونی SEM تأیید شد و نشان داده شد، نانوذرات تولید شده به وسیله گیاه نعناع در شرایط یکسان نسبت به اکالیپتوس بیشتر و پایدارتر بوده و این گیاه قابلیت بیشتری برای تولید نانوذرات نقره دارا بود. مورفولوژی ذرات سنتز شده به‌وسیله میکروسکوپ الکترونی عبوری TEM نیز تعیین و نانوذرات تشکیل شده کروی با اندازه 10 تا 20 نانومتر قابل مشاهده بودند. این نانوذرات نقره به عنوان ماده ضد باکتری دارای کاربردهای فراوانی در پزشکی و صنعت می‌باشند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Biological synthesis of silver nanoparticle based on Eucalyptus obliqua and Mentha spicata plant extracts and evaluation of their physical properties

نویسندگان [English]

  • Mansureh Rahmatyian 1
  • Mehdi Dadmehr 2
  • Mehdi Dadmehr 3

1 M.Sc., Department of Agriculture, Payame Noor University, Iran

2 Assistant Professor, Department of Biology, Payame Noor University, Iran

3 Assistant Professor, Department of Agriculture, Payame Noor University, Iran

چکیده [English]

Nanoparticles are one of the most applicable materials in, science such as biotechnology, and medicine due to their distinguished characters. The previous studies indicated that over the half of total production of nanoparticles is dedicated to the conventional and bio inspired synthesis of silver nanoparticles. These nanoparticles have antimicrobial characteristics and widespread applications in medicine and industry. Conventional chemical methods for synthesis of silver nanoparticles usually use the reducing agents that they are not compatible to environment and sometimes toxic and the resulting nanoparticles cannot be involved in biological applications. So, green synthesis of nanoparticles is the process that based on green chemistry for the synthesis of bio-nanoparticle is very important. In this study, eucalyptus (Eucalyptus obliqua) and mint (Mentha spicatae) extracts of medicinal plants for biological production of silver nanoparticles was used as a reducing agent. By adding the extract to the solution of silver nitrate at different temperatures and incubation times silver nanoparticles were produced. Changing the color from pale yellow to dark brown was showed the production of silver nanoparticles. There is also the strong peak absorption (SPR) at wavelengths between 420 and 460 with using spectroscopy UV/Vis which indicate the production of silver nanoparticles. The size of the nanoparticles was determined by electron microscopy analysis (SEM and TEM). The average sizes of nanoparticles in both plants were between 10 and 20 nm. The produced nanoparticles by the mint plant at the same condition was more stable than eucalyptus that confirmed that mint has more ability to produce silver nanoparticles.

کلیدواژه‌ها [English]

  • Silver nanoparticles
  • Biological synthesis
  • Eucalyptus
  • Mint
  • Antibacterial
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