افزایش غلظت فلاونوئیدهای ضد سرطانی روتین و کوئرستین موجود در Capparis spinosa تحت تأثیر برخی عوامل اکولوژیکی

نوع مقاله : علمی پژوهشی

نویسندگان

1 1دکتری بیوتکنولوژی گیاهی، گروه مهندسی تولید و ژنتیک گیاهی، دانشگاه زنجان

2 دانشیار گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی دانشگاه زنجان

3 استاد گروه ایمونولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی مازندران، ساری

4 دانشیار گروه علوم پایه دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری

5 استاد گروه شیمی دارویی، دانشکده داروسازی، دانشگاه علوم پزشکی مازندران، ساری

چکیده

فلاونوئیدهای گیاهی، پاسخ‌های مثبتی به تغییرات جغرافیایی به‌خصوص ارتفاع از سطح دریا نشان می‌دهند و با توجه به اینکه گیاه دارویی علف مار (Capparis spinosa) در رویشگاه‌های مختلف از لحاظ اکولوژیکی پراکنش دارد، لذا در این پژوهش تأثیر ارتفاع بر روی محتوای فلاونوئیدهای ضد سرطانی روتین و کوئرستین در گیاه دارویی علف مار مورد مطالعه قرار گرفت بدین‌صورت که از سه منطقه کوهستانی شهرستان آمل (نمارستاق، دلارستاق و بهرستاق) در چهار ارتفاع با اختلاف ارتفاع‌های 150 متری از هم (حداقل 850 و حداکثر 1650 متر) و با سه تکرار در قالب طرح کاملاً تصادفی در تابستان نمونه‌برداری انجام شد و از طریق روش اسپکتوفتومتری و HPLC، فلاونوئید کل، روتین و کوئرستین اندازه‌گیری و بررسی گردید. نتایج از نظر صفات مورد اندازه‌گیری، اختلاف معنی‌داری را در مناطق مختلف نشان داد (01/0p≤ و 05/0p≤)، به‌نحوی‌که بیشترین میزان فلاونوئید، روتین و کوئرستین در ارتفاع 1650 متر منطقه سوم (بهرستاق) به ترتیب با 17/0±51/4 میکرومول، 28/0±62/24 و 18/0±91/4 میلی‌گرم بر گرم وزن‌تر به‌دست آمد. لذا منطقه و ارتفاع کاملاً بر میزان متابولیت‌های دارویی علف مار تأثیر مثبت داشت. همچنین معادله خطوط رگرسیون و ضرایب تبیین (91/0r²=، 74/0r²= و 78/0r²=) نشان داد میزان فلاونوئید، روتین و کوئرستین در ارتفاع‌های مختلف مناطق نمارستاق، دلارستاق و بهرستاق با افزایش ارتفاع از سطح دریا روندی افزایشی داشته و شیب خط رگرسیون برای هر سه صفت مثبت بود. بنابراین طبیعت بهترین الگو برای به‌دست آوردن فلاونوئیدهای ضدسرطانی غلیظ‌تر و با ماهیت مطلوب‌تر در گیاهان است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Increasing the concentration of rutin and quercetin anti-cancer flavonoids in Capparis spinosa affected by some ecological factors

نویسندگان [English]

  • Ali Ghanbari 1
  • Mohammadreza Azimi 2
  • Alireza Rafiei 3
  • Puria Biparva 4
  • Mohammad Ali Ebrahimzadeh 5
1 Ph.D. of Plant Biotechnology, Department of Plant Production Engineering and Genetics, Zanjan University, Faculty of Agriculture, Zanjan, Iran
2 Associate Professor, Department of Plant Production Engineering and Genetics, Zanjan University, Faculty of Agriculture, Zanjan, Iran
3 Professor of Immunology, Mazandaran University of Medical Sciences, Sari, Iran
4 Associate Professor, Department of Basic Sciences, University of Agricultural Sciences and Natural Resources, Sari, Sari, Iran
5 Professor of Pharmaceutical Chemistry, Mazandaran University of Medical Sciences, Sari, Iran
چکیده [English]

Plant flavonoids show positive responses to geographical changes, especially altitude, and considering that the medicinal plant of capper (Capparis spinosa) is ecologically distributed in different habitats, so in this research the effect of altitude on the content of anti-cancer flavonoids such as rutin and quercetin were studied in capper which sampled in summer from three mountainous areas of Amol (Nemarestaq, Delarestaq and Behrestaq) at four altitudes with difference heights of 150 meters from each other (minimum 850 and maximum 1650 meters) and with three replications in a completely randomized design. Total flavonoids, rutin and quercetin were measured and analyzed by spectrophotometry and HPLC. The results showed a significant difference between measured traits in different regions (p≤0.01 and p≤0.05), so that the highest amount of flavonoids, rutin and quercetin were obtained at an altitude of 1650 m in the third region (Behrestaq) with 4.51±17 μmol, 24.62±0.28 and 4.91±0.18 mg/g fresh weight, respectively. Therefore, zone and altitude had a positive effect on the amount of medicinal metabolites of capper. Also, the equation of regression lines and explanatory coefficients (r² = 0.91, r² = 0.74 and r² = 0.78) showed that the amount of flavonoids, rutin and quercetin at different altitudes in Nemarestaq, Delarestaq and Behrestaq increased with increasing altitude and the slope of the regression line for all traits was positive. Therefore, nature is the best model for obtaining more concentrated and desirable anti-cancer flavonoids in plants.

کلیدواژه‌ها [English]

  • Altitude
  • Ecological
  • HPLC
  • Metabolite
  • Medicinal plant

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