آنالیز این‌سیلیکو اعضای خانواده ژنی CBL در گیاه هالوفیت آلوروپوس لیتورالیس و گیاه مدل آرابیدوپسیس تالیانا

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی کارشناسی‌ارشد بیوتکنولوژی کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ایران

2 دانشیار گروه بیوتکنولوژی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

3 استاد گروه مهندسی ژنتیک و بیولوژی، پژوهشکده ژنتیک و زیست‌فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری ، ایران

4 گروه مهندسی ژنتیک و بیولوژی، پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری

چکیده

پروتئین‌های شبه‌کالسینئورین B (CBLs) به‌عنوان یک مولکول پیام‌رسان ثانویه زیرخانواده گروه حس‌گرهای کلسیم بوده، و در تنظیم فرآیندهای فیزیولوژیکی و رشد و نمو گیاهان نقش مهمی دارند. در این مطالعه به منظور بررسی این‌سیلیکو و مقایسه خصوصیات پروتئین‌های دخیل در پیام رسانی کلسیم در گیاهان شورپسند و شیرین‌پسند، اعضای زیرخانواده ژنی CBL در دو گیاه آلوروپوس لیتورالیس و آرابیدوپسیس تالیانا مورد مطالعه قرار گرفت. بر اساس همولوژی توالی و روابط اورتولوژی با ژن‌های آرابیدوپسیس، شش ژن شناسایی شده در ژنوم آلوروپوس در قالب سه گروه پروتئینی AlCBL4، AlCBL2 وAlCBL10 دسته‌بندی شدند. بررسی خانواده ژنی AlCBL بر مبنای همردیفی چندگانه در گیاه آلوروپوس لیتورالیس موید حضور چهار دمین EF-hand در تمام این ژن‌ها بوده که ساختاری را برای اتصال یون کلسیم فراهم می‌نماید. شباهت بالای خصوصیات فیزیکوشیمیایی اکثر پروتئین‌های آلوروپوس به آرابیدوپسیس و داشتن رابطه قوی اورتولوژی با یکدیگر ممکن است دلالت بر محفوظ ماندن کارکرد و عملکرد این ژن‌ها در فرایند تکاملی بوده باشد. تجزیه و تحلیل الگوهای بیانی AtCBLs در اندام‌های مختلف و تحت تنش‌های غیر زنده مختلف نشان داد به دلیل انشقاق عملکردی و ساختاری، این ژن‌ها از الگوی بیان منحصربفردی برخوردارند. الگوی بیانی متفاوت در داده‌های ترانسکریپتوم AlCBLs نیز دلیلی بر الگوی عملکردی متفاوت این ژن‌هاست. نتایج بدست آمده در این تحقیق می‌تواند اطلاعات ارزشمندی در مورد نقش این خانواده ژنی و سازوکار‌های عملکردی آنها در تحمل به تنش‌های غیر زیستی برای مطالعات آتی مهیا سازد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

In silico analysis of CBL gene family in the halophyte plant Aeluropus littoralis and the model plant Arabidopsis thaliana

نویسندگان [English]

  • Mozhdeh Arab 1
  • Hamid Najafi Zarrini 2
  • Ghorbanali Nematzadeh 3
  • Seyyed Hamidreza Hashemi-petroudi 4
1 M.Sc. Student in Plant Breeding, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran.
2 Associated Professor, Department of Biotechnology, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran
3 Professors, Department of Genetic Engineering and Biology, Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU)
4 Department of Genetic Engineering and Biology, Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU)
چکیده [English]

Calcineurin B-like proteins (CBLs), which act as a secondary messenger molecule in the subfamily of the calcium sensor gene family, play a key role in regulating physiological processes, plant growth and development. In order to identification and comparison of proteins involving in calcium signaling in two model of halophyte and glycophyte plants, in silico analysis of the CBL gene family were done in Aeluropus littoralis and Arabidopsis thaliana. Based on sequence homology and orthological relationships with Arabidopsis genes, 6 genes identified in Aeluropus were classified into three protein groups: AlCBL4, AlCBL2 and AlCBL10. Multiple sequence allignment of the CBL gene family in Aeluropus littoralis confirmed the presence of four EF-hand domains in all genes, which provide a structure for calcium ion binding. The high similarity of the physicochemical properties of most Aeluropus proteins to Arabidopsis as well as the strong orthological relationship with each other may indicate the preservation of the function of these genes in the evolutionary process. Analysis of AtCBLs expression patterns in different organs/ abiotic stresses showed that these genes have unique expression profiles due to functional and structural convergent. Different expression profiles of AlCBLs in Aeluropus transcriptome would be an evidence for the functional divergent of these genes. The results obtained from this study can provide valuable information about the properties of this gene family and their functional roles in tolerating to abiotic stresses for future studies.

کلیدواژه‌ها [English]

  • Gene family
  • Calcium sensor
  • Halophyte
  • CBL
  • In silico analysis

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