تأثیر کاربرد نانوذرات تیتانیوم دی‌اکسید (TiO2 NPs) بر میزان ترکیبات پلی‌فنولی، فیتوشیمیایی و پروتئین SO6 ریشه‌های موئین Saponaria officinalis

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشیار ، مرکز علم و فناوری زیست شناسی، دانشکده علوم پایه، دانشگاه جامع امام حسین(ع)، تهران، ایران

2 دانشجوی مقطع دکتری ، مرکز علم و فناوری زیست‌شناسی، دانشکده علوم پایه، دانشگاه جامع امام حسین(ع)، تهران، ایران

3 دانشجوی مقطع دکتری، گروه بیوتکنولوژی کشاورزی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین‌المللی امام خمینی(ره) قزوین، ایران

4 استاد گروه بیوتکنولوژی دانشگاه پیام نور، تهران، ایران.

5 دانشجوی کارشناسی‌ارشد دانشگاه پیام نور، تهران، ایران.

چکیده

غاسول صابونی (Saponaria officinalis)، یک گیاه چند ساله از خانوده میخک است. سیستم کشت ریشه‌های موئین جایگزین مناسبی جهت بهبود تولید ترکیبات با ارزش دارویی می‌باشد. کاربرد گسترده فناوری نانو در کشت ریشه‌های موئین، بستری مناسب جهت تولید عناصر فعال می‌باشد. در این مطالعه، تأثیر غلظت‌های مختلف نانوذرات تیتانیوم دی‌اکسید (0، 10، 20، 30 و 50 میلی‌گرم بر لیتر) در زمان‌های (24 و 48 ساعت) بر میزان رشد، ظرفیت آنتی‌اکسیدانی، محتوای فنول و فلاونوئید کل، فعالیت آنزیم‌های آنتی‌اکسیدانی، برخی از ترکیبات پلی‌فنول و میزان پروتئین SO6 در ریشه‌های موئین غاسول صابونی بررسی شد. بیشترین و کمترین میزان وزن تر ریشه‌های موئین (به ترتیب 09/3 و 96/0 گرم) به ترتیب در محیط کشت‌های تیمار شده با 10 و 20 میلی‌گرم بر لیتر نانوذرات تیتانیوم دی‌اکسید و مدت زمان تیمار 48 و 24 ساعت مشاهده گردید. بیشترین میزان فنول کل (79/9 میلی‌گرم گالیک اسید بر گرم وزن تر) و فلاونوئید کل (06/1 میلی‌گرم کوئرستین بر گرم وزن تر) در ریشه‌های موئین تیمار شده با غلظت‌های 50 و 30 میلی‌گرم بر لیتر نانوذرات تیتانیوم دی‌اکسید و به ترتیب مدت زمان تیمار 24 و 48 ساعت به‌دست آمد. بیشترین میزان اکثر پلی‌فنول‌ها از جمله رزمارینیک اسید، سینامیک اسید و روتین در مدت زمان تیمار 24 ساعت تولید شد. بیشترین میزان پروتئین SO6 در غلظت 50 میلی‌گرم بر لیتر و مدت زمان 48 ساعت به‌دست آمد. با بهینه‌سازی آزمایشگاهی تولید ریشه مویین گیاه غاسول صابونی، می توان SO6 را در فرمانتور تولید نمود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of titanium dioxide nanoparticles (TiO2NPs) application on the polyphenolic, phytochemical and SO6 protein compounds content in Saponaria officinalis hairy roots

نویسندگان [English]

  • Hosein Honari 1
  • Ahad Hedayati 2
  • Zahra Moshiri 3
  • Mohammad Ali Ebrahimi 4
  • Afsaneh Manouchehri 5
1 Associate Professor, Biology Science and Technology Center, Faculty of Basic Sciences, Imam Hossein University, Tehran, Iran
2 Ph.D. Student, Biology Science and Technology Center, Faculty of Basic Sciences, Imam Hossein University, Tehran, Iran
3 Ph.D. Student, Department of Agricultural Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran
4 Professor , Student of Biotechnology, Payame Noor University, Tehran, Iran.
5 M.Sc. Student of Biotechnology, Payame Noor University, Tehran, Iran.
چکیده [English]

Saponaria officinalis is a perennial plant of the clove family. Hairy root culture system is a suitable alternative to improve the production of compounds with medicinal value. Extensive application of nanotechnology in hairy root culture is a suitable substrate for the production of active elements. In this study, the effect of different concentrations of titanium dioxide nanoparticles (0, 10, 20, 30 and 50 mg / l) and two exposure times (24 and 48 hours) on growth rate, antioxidant capacity, phenol and flavonoid content, antioxidant enzymes activity, polyphenol compounds content and the amount of SO6 protein in soapy hairy roots were investigated. The highest and lowest fresh weight of hairy roots (3.09 and 0.96 g, respectively) were found in culture medium treated with 10 and 20 mg / l titanium dioxide nanoparticles at 48 and 24 hours of exposure time, respectively. The highest total Phenol (9.79 mg GAE per g FW) and flavonoid (1.06 mg Que per g FW) content were observed in hairy roots treated with 50 and 30 mg / l titanium dioxide nanoparticles at 24 and 48 hours of exposure time respectively. The highest amount of most polyphenols including rosmarinic acid, cinnamic acid and rutin were achieved during 24 hours of treatment. The highest levels of SO6 protein was obtained in hairy roots treated with 50 mg/l titanium dioxide nanoparticles after 48 hours. By laboratory optimization of hairy root production of soap plant, SO6 can be produced in fermenter.

کلیدواژه‌ها [English]

  • Soap dipper
  • capillary root
  • titanium dioxide and SO6 protein

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