شناسایی و بررسی ارتباط عناصر تنظیمی ناحیه پروموتری خانواده ژنی DLV با بیان ژن در گیاه آلوروپوس لیتورالیس

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی دکتری بیوتکنولوژی کشارزی، گروه زراعت و اصلاح نباتات، دانشکده پردیس کشاورزی و منابع طبیعی کرج، دانشگاه تهران، کرج، ایران.

2 استادیار رشته اصلاح نباتات مولکولی، پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران.

3 دانشیار رشته بیوتکنولوژی گیاهی، گروه زراعت و اصلاح نباتات، دانشکده پردیس کشاورزی و منابع طبیعی کرج، دانشگاه تهران، کرج، ایران.

4 استادیار اصلاح نباتات مولکولی، پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران.

چکیده

در این پژوهش نوع و فراوانی عناصر تنظیمی در نواحی پروموتری خانواده ژنی DVL در گیاه آلوروپوس لیتورالیس مورد مطالعه قرار گرفت. همچنین بیان ژن AlDVL8 به‌عنوان یکی از اعضای این خانواده تحت تیمار تنش شوری، هورمون‌های ‌اسید‌سالیسیلیک، ‌اسید‌‌جیبرلیک و سیتوکینین اندازه‌گیری شد. نتایج بررسی پروموتری حاکی از آن بود که این خانواده ژنی عناصر تنظیمی متفاوتی برای پاسخ به انواع تنش‌ها و هورمون‌ها دارا می‌باشد. برخی از این عناصر تنظیمی در ناحیه پروموتری تمامی ژن‌ها موجودند که احتمالاً بیانگر نقش عمومی DVLها است. همچنین، گروهی از عناصر تنظیمی تنها در ناحیه پروموتری بعضی از ژن‌ها موجودند که می‌توانند مرتبط با فعالیت اختصاصی آن‌ها باشند. تیمارهای اعمال شده به غیر از سیتوکینین سبب افزایش بیان ژن در ‌اندام‌هوایی در زمان‌های 3، 12 و 24 ساعت و کاهش بیان در زمان 6 ساعت پس از اعمال تنش شد. تیمار سیتوکینین در همه زمان‌ها بیان ژن را در ‌اندام هوایی افزایش داد. در بافت ریشه تقریباً روند معکوس بیان ژن دیده شد، به صورتی که در زمان 6 ساعت افزایش بیان ژن و در زمان 12 و 24 ساعت کاهش بیان در تمامی تیمارها مشاهده شد. نتایج این تحقیق نشان داد که بیان ژن AlDVL8 در ‌اندام هوایی و زمینی با تیمارهای آزمایشی القا شد و بیان آن در دو بافت به‌صورت معکوس بود. با توجه به تغییرات هورمون‌ها طی تنش و القای بیان این خانواده ژنی و وجود عناصر پاسخ‌‌دهنده به تنش در نواحی پروموتری این ژن‌ها، این خانواده ژنی می‌تواند به‌عنوان کاندیدی برای تحمل به تنش‌ها پیشنهاد گردد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Identification and evaluation of relation between regulatory elements of the promoter region of DLV gene family and gene expression in Aeluropus littoralis

نویسندگان [English]

  • Maryam Chalekaei 1
  • Ali Dehestani 2
  • Ali reza Abbasi 3
  • Seyyed Hamidreza Hashemi-petroudi 4
1 Ph.D. Candidate of Agricultural Biotechnology, Department of Agronomy and Plant Breeding, Agricultural College, University of Tehran, Karaj, Iran.
2 Assistant Professor of Molecular Plant Breeding, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
3 Associate Professor, Department of Agronomy and Plant Breeding, Agricultural College, University of Tehran, Karaj, Iran.
4 Assistant professor, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
چکیده [English]

In this study, the type and frequency of regulatory elements in the promoter regions of DVL gene family in Aeluropus littoralis were studied. Relative expression of AlDVL8 gene as a member of this family was also measured under the salinity stress, salicylic acid, gibberellic acid and cytokinin. The results of the promoter study indicated that, this gene family has different regulatory elements for responding to stresses and hormones. Some of these regulatory elements are present in the promoter region of all genes, possibly indicating the general role of DVLs. Some others are present only in the promoter region of some genes that may be related to their specific activity. Treatments other than cytokinin increased gene expression in the shoot at 3, 12, and 24 hours and decreased expression at 6 hours. Cytokinin treatment at all times increased gene expression. In the root, almost the reverse trend of gene expression was observed, so that at 6 hours, increased gene expression was observed in all treatments, and at 12 and 24 hours, decreased expression was observed in all treatments. The results of this study showed that the expression of AlDVL8 gene in shoot and root organs was induced by experimental treatments and its expression was inverse in these two different tissues. Due to the changes in hormones during stress, expression induction of this gene family, and the presence of stress-responsive elements in the promoter regions of these genes, this gene family can be suggested as a candidate for stress tolerance.

کلیدواژه‌ها [English]

  • Gene expression
  • Gene family
  • Halophyte plant
  • Regulatory elements
  • Salt stress

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