شناسایی و گروه‌بندی ‏خانواده عوامل رونویسی WRKY در برنج ژاپونیکا

نوع مقاله : علمی پژوهشی

نویسندگان

1 گروه تولید و ژنتیک گیاهی، دانشگاه محقق اردبیلی، اردبیل، ایران.

2 گروه زیست‌شناسی سیستم‌ها، پژوهشگاه بیوتکنولوژی کشاورزی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران.

چکیده

خانواده‌ی ژنی WRKY رمزکننده گروه بزرگی از عوامل رونویسی هستند که در تنظیم ژن‌های پاسخ‌دهنده به تنش‌های زیستی و غیرزیستی دخیل می‌باشند. برای گردآوری اعضاء این خانواده ژنی در برنج ژاپونیکا (Oryza sativa ssp. Japonica)، جستجوی چندگانه در پایگاه‏های اطلاعاتی مختلف مرتبط انجام شد. به منظور شناسایی اعضای جدید،tblastn بر اساس توالی‏های حفاظت‏شده‏ی خانواده ژنیWRKY برنج در پایگاه اطلاعاتی NCBI و جستجو بر اساس مدل مخفی مارکوف صورت گرفت. همردیف سازی توالی‌های پروتئینی با استفاده از نرم افزار ClustalW و آنالیز درخت فیلوژنتیکی با استفاده از نرم‏افزار MEGA10 انجام گردید. براساس نتایج به‏دست آمده، 165 عضو از خانواده‏ی ژنی WRKY در برنج یافت شد که 63 عضو جدید بودند. توالی‌های موجود بر مبنای تعداد دمین‌های WRKY و ساختار انگشت روی در سه گروه اصلی دسته‌بندی شدند. بر این اساس 21 پروتئین در گروه I، 53 پروتئین با ساختار انگشت روی Cx7Cx23HxC در گروه III و 82 پروتئین با ساختار انگشت روی Cx4-5Cx22-23HxH در گروه II قرار گرفتند. مکان هر ژن روی کروموزوم مشخص شد. گروه‌های مختلف خانواده ژنی WRKY بر روی کروموزوم‌های مختلف برنج توزیع شده‌اند. بیشترین تعداد ژن OsWRKY روی کروموزوم یک (32 عضو) قرار داشتند. به دنبال بررسی‌های تکمیلی و تعیین ژن‌های کاندید امیدبخش دخیل در تحمل به هر یک از تنش‌ها، می توان از آنها در راستای افزایش تحمل به تنش‌های مورد نظر و تامین امنیت غذایی با استفاده از راهکارهای مهندسی ژنتیک یا اصلاح مولکولی بهره برد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Identification and classification of the WRKY transcription factors family in Japonica rice

نویسندگان [English]

  • Parisa Daryani 1
  • Fatemeh Farzaneh Piralger 1
  • Nasser Zare 1
  • Zahra-Sadat Shobbar 2
  • Rasool Asghari Zakaria 1
1 Department of Plant Production and Genetics, University of Mohaghegh Ardabili, Ardabil, Iran
2 Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
چکیده [English]

WRKY gene family encodes a large group of transcription factors regulating biotic and abiotic stress-responsive genes. In order to identify the WRKY gene family members in rice (Oryza sativa ssp. japonica), multiple searches were done in the related databases. Rice WRKY-conserved sequences were used as the templates for tBLASTN searches in datasets for finding new members. An HMM profile of WRKY domain was also used to find WRKY gene family. Multiple sequence alignment was done using clustalW software, and phylogenetic trees were drawn using MEGA10 software based on a neighbour-joining method with a 1000 repeats bootstrap index. According to the results, 165 members of the WRKY gene family were found in rice, of which 63 were new members. Sequences were divided into three main groups based on the number of WRKY domains and the structure of zinc-finger motifs. Conclusively, there were 21 proteins with two WRKY conserved domains in group I, 53 proteins with one WRKY conserved domain and Cx7Cx23HxC zinc-finger motif in group III and 82 proteins with one WRKY conserved domain and Cx4-5Cx22-23HxH zinc-finger motif in group II. The chromosomal location of OsWRKYs was detected on the rice genome. The different groups were distributed on various chromosomes. The greatest number of OsWRKY genes (32 members) were located on chromosome 1. Following complementary research and identification of promising candidate genes involved in tolerance to each stress, they can be used to increase tolerance to the desired stresses and provide food security using genetic engineering or molecular breeding approaches.

کلیدواژه‌ها [English]

  • Oryza sativa
  • Japonica subspecies
  • Phylogenetic analysis
  • Transcription factors
  • WRKY

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