شناسایی نشانگرهای CBDP و SCoT مرتبط با بیماری‌های زنگ‌برگی، سفیدک پودری و لکه برگی سپتوریایی در ژنوتیپ‎های گندم بهاره

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی کارشناسی‌ارشد بیوتکنولوژی، گروه تولیدات گیاهی، دانشکده علوم کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس، گنبد، ایران.

2 استادیار، گروه تولیدات گیاهی، دانشکده علوم کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس، گنبد، ایران.

3 دانشیار، گروه تولیدات گیاهی، دانشکده علوم کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس، گنبد، ایران.

4 مربی پژوهش بیماری‌شناسی، مرکز تحقیقات کشاورزی و منابع طبیعی گنبد کاووس، گنبد، ایران.

چکیده

بیماری‌های قارچی برگ گندم از جمله مهم‌ترین عوامل کاهش عملکرد این محصول استراتژیک می‌باشند. بنابراین شناسایی منابع مقاومت به بیماری‌های مهم قارچی گندم از اهمیت بالایی برای افزایش عملکرد این محصول برخوردار است. به منظور شناسایی نشانگرهای حاوی اطلاعاتCBDP و SCoT برای بیماری‏های زنگ‌برگی، سفیدک پودری و سپتوریوز برگی، 63 ژنوتیپ گندم به صورت طرح اگمنت در ایستگاه تحقیقات کشاورزی عراقی محله(گرگان)، در سال زراعی 98-1397 کاشته شدند. ژنوتیپ‎ها با استفاده از تجزیه خوشه‎ای و براساس روش UPGMA به سه گروه دسته‎بندی شدند که این گروه‌ها به ترتیب 24، 17 و 22 عضو داشتند. از بین ژنوتیپ‌های مورد بررسی ژنوتیپ‌های شماره 49 و 60 مقاومت بالایی نسبت به هر سه بیماری نشان دادند. نتایج تجزیه ارتباط نشان داد که در مجموع از 16 جفت آغازگر به کار رفته، 99 آلل تکثیر شدند که از بین آنها 43 الل چندشکلی نشان دادند. بیشترین درصد چندشکلی مربوط به آغازگر نوع SCoT و کم‌ترین آن مربوط به آغازگر CBDP بود. و نشانگرهای SCoT11-2 و CBDP 10-2 با هر سه بیماری ارتباط معنی‌داری نشان دادند. همچنین نشانگر SCoT21-3 با دو بیماری سفیدک پودری و زنگ قهوه‏ای ارتباط معنی‎داری (05/0P

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Investigation of informative CBDP and SCoT markers for leaf rust, powdery mildew and septoria leaf blotch diseases in spring wheat genotypes

نویسندگان [English]

  • Behzad Naeimi Nezamabad 1
  • Fakhtak Taliei 2
  • Abbas Biabani 3
  • Hossein Sabouri 3
  • Masume Kheirgu 4
1 M.Sc. Student, Department of Plant Production, College of Agriculture and Natural Resources, University of Gonbad Kavous, Gonbad, Iran.
2 Assistant Professor, Department of Plant Production, College of Agriculture and Natural Resources, University of Gonbad Kavous, Gonbad, Iran.
3 Assistant Professor, Department of Plant Production, College of Agriculture and Natural Resources, University of Gonbad Kavous, Gonbad, Iran.
4 Department of Horticulture and crop research, Golestan Kavous Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Gonbad Kavous, Iran.
چکیده [English]

Fungal leaf diseases are the most destructive factors to wheat (Triticum aestivum L.) yield. Therefore, identification of resistance sources to important fungal diseases is a great importance to increase wheat yield. In order to identify informative CBDP and SCoT markers for leaf rust, powdery mildew and septoria leaf blotch disease, 63 wheat genotypes were planted through the augment design in agricultural research station of Araghimahaleh (Gorgan) during 2018-19. Clustering based on UPGMA, put the genotypes into 3 groups with 24, 17 and 22 individuals. Among the studied genotypes, genotypes No. 49 and 60 showed highest resistance to all three diseases. Association analysis showed that from 16 CBDP and SCoT primers, 99 bands produced in total, which 43 bands were polymorph. The maximum percent of polymorphism belonged to SCoT primers and the minimum polymorphism belonged to CBDP. SCoT11-2, CBDP 10-2 was shown significant correlation with all three diseases. SCoT21-3 was also significantly (P

کلیدواژه‌ها [English]

  • Wheat
  • Leaf diseases
  • CBDP and SCoT markers
Adhikari TB, Mamidi S, Gurung S, Bonman JM (2015) Mapping of new quantitative trait loci (QTL) for resistance to Septoria tritici blotch in spring wheat (Triticum aestivum L.). Euphytica. 205(3):699-706.
Ahmadi M, Fazeli A, Arminian A (2017) Identification of Informative ISSR Marker Linked to Resistance to Powdery Mildew in Barley (Hordeum vulgare) at Adult Growth Stage. J Crop Breed. 9(22): 31-40.
An ZW, Xie LL, Cheng H, Zhou Y, Zhang Q, He XG, Huang HS (2009) A silver staining procedure for nucleic acids in polyacrylamide gels without fixation and pretreatment. Anal. Biochem. 391(1):77-79.
Arraiano LS, Brown JK. (2017) Sources of resistance and susceptibility to Septoria tritici blotch of wheat. Mol. plant pathol. 18(2):276-92.
Autrique E, Tanksley SD, Sorrells ME, Singh RP (1995) Molecular markers for four leaf rust resistance genes introgressed into wheat from wild relatives. Genome. 38 (1): 75-83.
Brown JKM, Chartrain L, Lasserre‐Zuber P, and Saintenac C (2015) Genetics of resistance to Zymoseptoria tritici and applications to wheat breeding. Fungal Genet. Biol. 79, 33–41.
Buerstmayr H, Lemmens M, Berlakovich S, Ruckenbauer P (1999) Combining ability of resistance to head blight caused by Fusarium culmorum (W.G. Smith) in the F1 of a seven parent diallel of winter wheat (Triticum aestivum L.). Euphytica. 110: 199-206.
Collard BC, Mackill DJ (2009) Start Codon Targeted (SCoT) polymorphism: a simple novel DNA marker technique for generating gene-targeted markers in plants. Plant Mol Biol Rep. 27(1): 86–93
Coventry DR, Yadav A, Poswal RS, Sharma RK, Gupta RK, Chhokar RS, Gill SC, Kumar V, Kumar A, Mehta A, Kleemann SG (2011)  Irrigation and nitrogen scheduling as a requirement for optimising wheat yield and quality in Haryana, India. Field Crops Res. 123(2): 80-88.
Czembor PC, Radecka-Janusik M, Mańkowski D (2011) Virulence spectrum of Mycosphaerella graminicola isolates on wheat genotypes carrying known resistance genes to Septoria tritici blotch. Phytopath. 159(3): 146-154.
Dadrezaei ST, Tabatabai N, Lakzadeh I, Jafarnezhad A, Afshari F, Hassan Bayat Z (2018) Evaluation of tolerance to leaf rust disease in some selected bread wheat genotypes. Appl. Entomol. Phytopathol. 86(1): 29-40. 
Dakouri A, McCallum B, Walichnowski A, and Cloutier S (2010) Fine-mapping of the leaf rust Lr34 locus in Triticum aestivum (L.) and characterization of large germplasm collections support the ABC transporter as essential for gene function. Theor. Appl. Genet. 121: 373–384.
Dehghan M (2009) Resistant genotypes of preliminary, advanced and invigorating climate, hot and humid north to powdery mildew in wheat. (Research Project Final Report). Publication of Agriculture and Natural Resources, Research Center of Golestan, 15 p.
Ebrahimyan M, Nasrollahnezhad Ghomi AA, Null K, Ramezanpour SS (2019) Evaluation of resistance to leaf rust at adult stage in some bread wheat cultivars. J. Plant Product. Res. 26(3):89-102.
Etminan A, Pour-Aboughadareh A, Mohammadi R, Noori A, Ahmadi-Rad A (2018) Applicability of CAAT boxderived polymorphism (CBDP) markers for analysis of genetic diversity in durum wheat. Cereal Res Commun. 46(1): 1–9.
Eyal Z, Scharen AL, Prescott JM, and Van Ginkel M (1987) The Septoria Disease of Wheat: Concepts and Methods of Disease Management. CIMMYT Mexico, D. F.
Fabriki Ourang S, Golmohammadie M, Karimi H (2019) Evaluation of genetic relationships among promising and commercial olive varieties using gene-targeted CAAT box-derived polymorphism (CBDP) markers. J. Agric. Biotech. 10(4): 93-109.
Farshadfar M, Shirvan, H, Amjadian M, Yaghotipoor A (2017) Application of SCoT marker to discriminate Lolium perenne and Lolium multiflorum species. Iranian Journal of Rangelands and Forests Plant Breed. and Gene. Res. Vol. 26(1):207-220.
Figlan S, Ntushelo K, Mwadzingeni L, Terefe T, Tsilo TJ, Shimelis H (2020) Breeding Wheat for Durable Leaf Rust Resistance in Southern Africa: Variability, Distribution, Current Control Strategies, Challenges and Future Prospects. Front. Plant Sci. 11:549.
Ghazvini H, Sarhangi M, Afshari F (2018) Identification of molecular markers linked to Lr34/Yr18 gene and evaluation of resistance to leaf rust and yellow rust in wheat (Triticum aestivum L.) cultivars and promising lines. Iranian J, Crop Sci. 20(2):108-25.
Goudemand E, Laurent V, Duchalais L, Ghaffary SM, Kema GH, Lonnet P, Margalé E, Robert O (2013) Association mapping and meta-analysis: two complementary approaches for the detection of reliable Septoria tritici blotch quantitative resistance in bread wheat (Triticum aestivum L.). Mol. Plant Breed. 32(3): 563-584.
Hamidi H, Talebi R, Keshavarz F (2014) Comparative efficiency of functional gene based markers, start codon targeted polymorphism (SCoT) and conserved DNA-derived Polymorphism (CDDP) with ISSR markers for diagnostic fingerprinting in wheat (Triticum aestivum L.). Cereal Res Commun 42(4): 558–567.
Heidari P, Etminan A, Azizinezhad R, Khosroshahli M (2017) Genomic variation studies in durum wheat (Triticum turgidum ssp. durum) using CBDP, SCoT and ISSR markers. Indian J Genet Pl Br. 77(3): 379–386.
IBM Corp. Released (2010) IBM SPSS Statistics for Windows, Version 19.0. Armonk, NY: IBM Corp.
Jaczewska-Kalicka A, & Krasinski T (2010) Pathogenic fungi dominating in winter wheat in the years 2006-2009. Prog. Plant Prot.50: 642-647.
Jia M, Xu H, Liu C, Mao R, Li H, Liu J, Du W, Wang W, Zhang X, Han R, Wang X (2020) Characterization of the powdery mildew resistance gene in the elite wheat cultivar Jimai 23 and its application in marker-assisted selection. Frontiers in genetics. 2;11:241.
Kalendar R (2007) FastPCR: a PCR primer designand repeat sequence searching software with additional tools for the manipulation and analysis of DNA and protein. Available atwww.biocenter.helsinki.fi/programs/fastpcr.htm; 2007.
Kara K, Mezghani N, Debbabi OS, Madini M, Rached-Kanouni M, Naceur MB (2016) Assessment of genetic diversity of wheat (Triticum aestivum L.) using agro-morphological characters and microsatellite markers. Int J Biosci. 9: 92-101.
Kheirgu M, Panjekeh N, Taliey F (2020) Identification of New Sources of Resistance to Zymoseptoria tritici Blotch in Genotypes of Spring Bread Wheat. J. Appl. Res. Plant Protect. 9(2):31-43.
Kolmer J (2013) Leaf rust of wheat: pathogen biology, variation and host resistance. Forests. 4(1): 70–84.
Li G, Fang T, Zhang H, Xie C, Li H, Yang T, Nevo E, Fahima T, Sun Q, Liu Z (2009) Molecular identification of a new powdery mildew resistance gene Pm41 on chromosome 3BL derived from wild emmer (Triticum turgidumvar. Dicoccoides). Theo Appl Genet. 119(3): 531–539.
Li GQ, Cowger C, Wang XW, Carver BF, and Xu XY (2019). Characterization of Pm65, a new powdery mildew resistance gene on chromosome 2AL of a facultative wheat cultivar. Theor. Appl. Genet. 132, 2625–2632.
Masoudi H, Sabouri H, Taliei F, Jafarbay J (2016) Investigation of Genetic Diversity and Relationship for Morphophenological Traits and Resistance to Powdery mildew in Wheat Germplasm Using IRAP, ISSR and iPBS Markers. J Crop Sci Biotech. 7 (18): 41-56.
McCartney, C, Somers D, McCallum B, Thomas J, Humphreys D, Menzies J, Brown P (2005) Microsatellite tagging of the leaf rust resistance gene Lr16 on wheat chromosome 2BSc. Mol. Breed. 15(4): 329-337.
Mekonnen T, Haileselassie T, Kaul T, Sharma M, Geleta B, Tesfaye K (2019) Molecular screening of Zymoseptoria tritici resistance genes in wheat (Triticum aestivum L.) using tightly linked simple sequence repeat markers. Europe. J. Plant Pathol. 155(2):593-614.
Mohammadbeygi A, Roohparvar R, Torabi M (2014) Resistance sources to Septoria leaf blotch in selected wheat genotypes. Seed Plant Improv. J. 30(3):605-621.
Motlagh SF, Rohparvar R, Kia S, Zamanizadeh HR (2015) Evaluation of resistance of some wheat cultivars and lines to septoria leaf blotch at seedling and adult plant stages. Seed and Plant Improve. J 31(3): 509-529.
Pour-Aboughadareh A, Etminan A, Shooshtari L, Maleki-Tabrizi N (2019) Comparative Assessment of SCoT and CBDP Markers for Investigation of Genetic Diversity Existing in Different Aegilops Species. Agric. Biotechnol. 11 (4): 153-174.
Prabhu K, Gupta S, Charpe A, Koul S (2004) SCAR marker tagged to the alien leaf rust resistance gene Lr19 uniquely marking the Agropyron elongatum‐derived gene Lr24 in wheat: a revision. Plant Breed. 123(5): 417-420.
Prins R, Groenewald J, Marais G, Snape J, Koebner R (2001) AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat. Theor. Appl. Genet. 103(4): 618-624.
Qiu L, Wang H, Li Y, Wang W, Liu Y, Mu J, Geng M, Guo W, Hu Z, Ma J, Sun Q (2020) Fine mapping of the wheat leaf rust resistance gene LrLC10 (Lr13) and validation of its co-segregation markers. Frontiers in Plant Sci.;11:470.
Qureshi N, Bariana H, Kumran VV, Muruga S, Forrest KL, Hayden MJ, et al (2018) A new leaf rust resistance gene Lr79 mapped in chromosome 3BL from the durum wheat landrace Aus26582. Theor. Appl. Genet. 131, 1091–1098.
Roelfs AP, Singh RP, Saari EE (1992) Rust diseases of wheat: concepts and methods of diseases Management. CIMMYT, Mexico, DF. 81p.
Saari EE, Prescott JM (1975) A scale for appraising the foliar intensity of wheat disease. Plant Disease Reporter. 59: 377-380.
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA sepacer length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc. Natl. Acad. Sci. 81(24): 8014–8019.
Saravani S, Sabouri H, Taliei F, Biabani A, Rahemi Karizaki, A (2018) Evaluation of genetic diversity of wheat genotypes in terms of powdery mildew resistance, grain yield, yield components and molecular markers. J. Agric. Biotech. 11 (3): 57-82.
Shahraki M, Imam Juma AA, Fakheri BA, Fazeli Nasab B (2017) 'Identification of diversity of Sistan wheat cultivars in terms of genes resistant to wheat rust diseases with microsatellite markers', J Crop Sci Biotechnol. 8 (23): 57-58.
Simón MR, Cordo CA, Castillo NS, Struik PC, Börner A (2012) Population structure of Mycosphaerella graminicola and locatio of genes for resistance to the pathogen: recent advances in Argentina. Int J Agron 1–7.
Singh AK, Rana MK, Singh S, Kumar S, Kumar R, Singh R (2014) CAAT box-derived polymorphism (CBDP): a novel promoter-targeted molecular marker for plants. J. Plant Biochem. Biotech 23: 175–183.
Tanveer H, Ishtiaq M, Mehrban A, Waheeda M, Shahzad A, Mehwish M (2016) Assessment of wheat foliar mycoflora and its management strategies in district bhimber, azad kashmir, pakistan. Appl. Ecol. Environ. Res. 14(5): 49-65.
Terefe T, Pretorius Z, Bender C, Visser B, Herselman L, Negussie T (2011) First report of a new wheat leaf rust (Puccinia triticina) race with virulence for Lr12, 13, and 37 in South Africa. Eur. J. Plant Pathol. 139: 95–105.
Tomkowiak A, Skowrońska R, Buda A, Kurasiak-Popowska D, Nawracała J, Kowalczewski PŁ, Pluta M, Radzikowska D (2019) Identification of leaf rust resistance genes in selected wheat cultivars and development of multiplex PCR. Open Life Sciences. 14(1):327-34.
Vanda M, Hoshmand S (2011) Genetic analysis of grain yield and related traits in durum genotypes using diallel. Iranian J Crop Sci. 13: 206-218.
Vechet L (2006) Reaction of winter wheat cultivars and breeding lines to Blumeria graminis f.sp. tritici. Plant Protect. Sci. 42: 15–20.
Yeh FC, Yang RC, Boyle TJB, Ye ZH, Mao JX (1997) POPGENE, The user-friendly shareware for population genetic analysis. Edmonton, Molecular Biology and Biotechnology Center, University of Alberta, Canada.