با همکاری مشترک دانشگاه پیام نور و انجمن بیوتکنولوژی جمهوری اسلامی ایران

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی کارشناسی‌ارشد بیوتکنولوژی، گروه تولیدات گیاهی، دانشکده علوم کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس، گنبد، ایران.

2 استادیار، گروه تولیدات گیاهی، دانشکده علوم کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس، گنبد، ایران.

3 دانشیار، گروه تولیدات گیاهی، دانشکده علوم کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس، گنبد، ایران.

4 مربی پژوهش بیماری‌شناسی، مرکز تحقیقات کشاورزی و منابع طبیعی گنبد کاووس، گنبد، ایران.

چکیده

بیماری‌های قارچی برگ گندم از جمله مهم‌ترین عوامل کاهش عملکرد این محصول استراتژیک می‌باشند. بنابراین شناسایی منابع مقاومت به بیماری‌های مهم قارچی گندم از اهمیت بالایی برای افزایش عملکرد این محصول برخوردار است. به منظور شناسایی نشانگرهای حاوی اطلاعاتCBDP و SCoT برای بیماری‏های زنگ‌برگی، سفیدک پودری و سپتوریوز برگی، 63 ژنوتیپ گندم به صورت طرح اگمنت در ایستگاه تحقیقات کشاورزی عراقی محله(گرگان)، در سال زراعی 98-1397 کاشته شدند. ژنوتیپ‎ها با استفاده از تجزیه خوشه‎ای و براساس روش UPGMA به سه گروه دسته‎بندی شدند که این گروه‌ها به ترتیب 24، 17 و 22 عضو داشتند. از بین ژنوتیپ‌های مورد بررسی ژنوتیپ‌های شماره 49 و 60 مقاومت بالایی نسبت به هر سه بیماری نشان دادند. نتایج تجزیه ارتباط نشان داد که در مجموع از 16 جفت آغازگر به کار رفته، 99 آلل تکثیر شدند که از بین آنها 43 الل چندشکلی نشان دادند. بیشترین درصد چندشکلی مربوط به آغازگر نوع SCoT و کم‌ترین آن مربوط به آغازگر CBDP بود. و نشانگرهای SCoT11-2 و CBDP 10-2 با هر سه بیماری ارتباط معنی‌داری نشان دادند. همچنین نشانگر SCoT21-3 با دو بیماری سفیدک پودری و زنگ قهوه‏ای ارتباط معنی‎داری (05/0P

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigation of informative CBDP and SCoT markers for leaf rust, powdery mildew and septoria leaf blotch diseases in spring wheat genotypes

نویسندگان [English]

  • Behzad Naeimi Nezamabad 1
  • Fakhtak Taliei 2
  • Abbas Biabani 3
  • Hossein Sabouri 3
  • Masume Kheirgu 4

1 M.Sc. Student, Department of Plant Production, College of Agriculture and Natural Resources, University of Gonbad Kavous, Gonbad, Iran.

2 Assistant Professor, Department of Plant Production, College of Agriculture and Natural Resources, University of Gonbad Kavous, Gonbad, Iran.

3 Assistant Professor, Department of Plant Production, College of Agriculture and Natural Resources, University of Gonbad Kavous, Gonbad, Iran.

4 Department of Horticulture and crop research, Golestan Kavous Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Gonbad Kavous, Iran.

چکیده [English]

Fungal leaf diseases are the most destructive factors to wheat (Triticum aestivum L.) yield. Therefore, identification of resistance sources to important fungal diseases is a great importance to increase wheat yield. In order to identify informative CBDP and SCoT markers for leaf rust, powdery mildew and septoria leaf blotch disease, 63 wheat genotypes were planted through the augment design in agricultural research station of Araghimahaleh (Gorgan) during 2018-19. Clustering based on UPGMA, put the genotypes into 3 groups with 24, 17 and 22 individuals. Among the studied genotypes, genotypes No. 49 and 60 showed highest resistance to all three diseases. Association analysis showed that from 16 CBDP and SCoT primers, 99 bands produced in total, which 43 bands were polymorph. The maximum percent of polymorphism belonged to SCoT primers and the minimum polymorphism belonged to CBDP. SCoT11-2, CBDP 10-2 was shown significant correlation with all three diseases. SCoT21-3 was also significantly (P

کلیدواژه‌ها [English]

  • Wheat
  • Leaf diseases
  • CBDP and SCoT markers
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