بررسی تأثیر سولفورافان حاصل از عصاره گیاه Brassica oleracea بر بیان دو ژن‌NF-KB و STAT3 ، مؤثر در مسیر التهاب سیستم ایمنی

نوع مقاله : علمی پژوهشی

نویسندگان

1 کارشناس‌ارشد، گروه بیوتکنولوژی کشاورزی، دانشگاه پیام نور، تهران، ایران.

2 استادیار، گروه بیوتکنولوژی کشاورزی، دانشگاه پیام نور، تهران، ایران.

چکیده

التهاب، پاسخ طبیعی سیستم ایمنی بدن در برابر آسیب یا بیماری به عنوان یک مکانیسم دفاعی، در بدن است. سیستم ایمنی بدن سلول‌های آسیب دیده، محرک‌ها و پاتوژن‌ها را شناسایی کرده و روند بهبودی را آغاز می‌کند که این روند ممکن است باعث بروز التهاب در بدن شود. گاهی اوقات التهاب طولانی مدت و پاسخ‌‌های ایمنی بیش از حد معمول، باعث بروز عوارض و آسیب‌های بیشتر و بروز بیماری‌های مزمن می‌شود. در این پژوهش عصاره Brassica oleracea استخراج و وجود سولفورافان با روش GC-MSو MTTدر آن تأیید شد. میزان سمیت عصاره استخراج شده بر لاین‌های سلولی، رد شد، بر بیان ژن‌های STAT3 و NF-KB مؤثر در مسیر التهاب سیستم ایمنی مورد بررسی قرار گرفت. از دو ژن GAPDH و β-actin به عنوان ژن‌های کنترل داخلی، در لاین‌ سلولی‌ عصبی 1321N1 و لاین سلولی HFF به عنوان سلول نرمال استفاده شد. سلول‌ها با دو میلی‌گرم از عصاره به مدت سه روز تیمار شدند و تغییرات سیستم ایمنی نیز در بیان ژن‌ها، مورد بررسی قرار گرفت. نتایج نشان داد که سولفورافان باعث کاهش بیان ژن‌های NF-KB و STAT3 شد و هر دو ژن از مهم‌ترین واسطه‌های التهاب می‌باشند. بر اساس نتایج بدست آمده در این پژوهش، استفاده از ماده مؤثره سولفورافان می‌تواند در کاهش علل ایجاد مشکلات بیماران دارای التهاب سیستم ایمنی مؤثر باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigation of the Effect of Sulforaphane Content of Brassica oleracea Extract on the Expression of NF-κB and STAT3 Genes Contributing Affecting the Inflammatory Response Pathway of Immune System

نویسندگان [English]

  • Mahdieh Shojaee 1
  • Razieh Sarabadani 2
1 M.Sc., Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran.
2 Assistant Professor, Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran.
چکیده [English]

Inflammation is a natural response of the body’s immune system to disease, protecting the body in the form of a defensive mechanism. The immune system identifies the damaged body cells, stimuli, and pathogens to trigger the healing process – a mechanism that can engage the body with inflammation. In some cases, too long inflammation and excessive immune responses led to side effects and damages that dominate their benefits, leading to chronic inflammation and then engagement with different diseases. In the present research, effect of the broccoli sprouts extract on the expression of the NF-κB and STAT3 genes affecting the inflammatory response pathway of immune system was investigated. Results of gas chromatography – mass spectroscopy (GC-MS) and MTT assay confirmed the presence of the sulforaphane in the extract while rejecting their toxicity to cell lines. Moreover, the GAPDH and β-actin genes were used as internal control genes in the 1321N1 neural cell line, with the HFF cell line used as normal cell. The cells were treated with 2 mg of the extract for 3 days, during which time the changes in the immune system in terms of the genes expressions were monitored. The results showed that the sulforaphane attenuate the expressions of the NF-κB and STAT3 genes as two of the most important inflammatory mediating genes. It seems that the use of sulforaphane as active ingredient can effectively attenuate the cause of the disorders faced by the patients with immune system inflammation.

کلیدواژه‌ها [English]

  • broccoli
  • sulforaphane
  • immune system inflammation
  • NF-&kappa
  • B
  • STAT3

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