نوع مقاله : علمی پژوهشی
نویسندگان
1 کارشناسی‌ارشد بیوتکنولوژی کشاورزی، دانشکده مهندسی انرژی و فناوری‌های نوین دانشگاه شهید بهشتی، تهران.
2 دانشیار گروه بیوتکنولوژی، دانشکده مهندسی انرژی و فناوری‌های نوین دانشگاه شهید بهشتی، تهران.
3 دانشیار بخش تحقیقات دانههای روغنی موسسه تحقیقات اصلاح و تهیه نهال و بذر، کرج.
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Soil and water salinity is one of the limiting factors for rice cultivation in the worldwide. Among crops, rice is very sensitive to salinity but the sensitivity is higher at the seedling and heading stages. Study of gene expression patterns besides away of cellular proteins function, could be useful to create of resistance plants to stress such as salinity. In this study, expression of the Coumaroyl coenzyme A -3hydroxylase (C3H) gene and physiological processes was surveyed under salinity stress in Oryza sativa cv. IR65192-4B cultivar, by Complete Randomized Design (CRD) in three replicates and qRT-PCR technique. Physiological results showed that, salinity stress reduced root length in 100mM but not significant in 0 and 50 mM NaCl. In addition, reduction of root dry weight was significant under salinity. On the other hand, data analysis of C3H gene expression shown, expression altered in differentages of the rice roots under one level of NaCl, and also, is variable in one age but in different of NaCl concentrations. Weak gene performance in one branch can lead the loss of other branches efficiency. Therefore, this point must be consider to create of salt resistance plant because can play a major role in overall resistance.
کلیدواژه‌ها [English]
)