با همکاری مشترک دانشگاه پیام نور و انجمن بیوتکنولوژی جمهوری اسلامی ایران

نوع مقاله : علمی پژوهشی

نویسندگان

گروه علوم و زیست‌فناوری گیاهی، دانشکده علوم و فناوری زیستی‌، دانشگاه شهید بهشتی‌، تهران، ایران.

چکیده

کالمودولین به‌معنای پروتئین تنظیم‌شده کلسیم است و به پروتئین داخل سلولی کوچکی گفته‌می‌شود که خاصیت اتصال به یون کلسیم و ایجاد واسطه در بسیاری از اقدامات داخل سلولی آن را دارد. فاکتورهای فعال کننده‌های رونویسی متصل‌شونده به کالمودولین (CAMTA) به‌عنوان یکی از پروتئین‌های پاسخ‌دهنده به استرس شناخته شده‌اند. در این مطالعه ژن‌های خانواده CAMTA در گیاه ذرت انتخاب شدند و توزیع کروموزومی، ساختار ژن، الگوهای دمین، درخت فیلوژنتیکی ژن‌های CAMTA در ذرت برای بررسی بیشتر عملکرد آنها مورد آنالیز قرار گرفت. همچنین برای شناسایی الگوی بیان در بافت‌های مختلف گیاه ذرت، آنالیز بیان ژن‌های ZmCAMTA در پاسخ به تنش گرما و جوانه‌زنی مشخص شد. ژن‌های ZmCAMTA1 و ZmCAMTA2 در تمام مراحل رشدی گیاه بیان نشان دادند. ساختار ژن در اکثر پروتئین‌ها مشابه بود و طبقه‌بندی فیلوژنتیکی CAMTA را تأیید می‌کند. پیش‌بینی سیس المنت‌ها در ناحیه پروموتری ژن‌ها نشان داد که bZIP و AP2/ERF بالاترین سیس المنت در ناحیه پروموتری ژن‌های ZmCAMTA را به خود اختصاص دادند. در بافت برگ در پاسخ به تنش گرمایی ژن ZmCAMTA1 افزایش بیان نشان داد. در صورتی‌که ژن ZmCAMTA2 در بافت ساقه در پاسخ به تنش گرمایی افزایش بیان نشان داد. ژن ZmCAMTA2 در پاسخ به جوانه‌زنی افزایش بیان نشان داد. این مطالعه می‌تواند به‌عنوان یک منبع مفید برای مطالعات بیشتر ZmCAMTA در آینده در گونه‌های مختلف گیاهی در نظر گرفته شود و اطلاعات مفیدی برای یافتن ژن کاندیدا در پاسخ به تنش فراهم کند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

CAMTA gene family analysis in maize and expression of genes responsive to heat stress and germination

نویسندگان [English]

  • Abbas Saidi
  • Zohreh Hajibarat

Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.

چکیده [English]

Calmodulin is a regulated protein of calcium and is a small intracellular protein that binds to calcium ions and mediates many of its intracellular actions. Calmodulin-binding transcription factors (CAMTAs) are recognized as one of the stress-responsive proteins. In this study, CAMTA genes were selected in maize. In this study, CAMTA family genes in maize were selected and chromosomal distribution, gene structure, domain patterns, and phylogenetic tree of CAMTA genes in maize were analyzed to further evaluate. To identify expression levels in different plant tissues, CAMTA gene expression analysis in response to heat stress and germination was studied. ZmCAMTA1 and ZmCAMTA2 genes were expressed in heat stress. Gene structure was similar in most proteins in each group, confirming the phylogenetic classification of CAMTA. Prediction of cis-elements in the promoter region of genes showed that bZIP and AP2 / ERF had the highest cis-elements in the promoter region of ZmCAMTA genes. In leaf tissue, ZmCAMTA1 gene was up-regulated expression in response to heat stress. ZmCAMTA2 gene was up-regulated in stem tissue in response to heat stress. The ZmCAMTA2 gene in response to increased expression germination showed that this study could be considered as a useful resource for future comparative studies of ZmCAMTA in different plant species and provide useful information for finding candidate genes in response to stress.

کلیدواژه‌ها [English]

  • Calmodulin-binding transcription factors
  • gene expression
  • heat stress
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