شناسایی و مطالعه کارکردی خانواده ژنی لیپواکسیژناز (LOX) در گستره ژنوم برخی از گونه‌های بقولات بر پایه روش‌های بیوانفورماتیکی

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی دکتری بیوتکنولوژی کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران

2 استاد، گروه تولید و ژنتیک گیاهی، دانشگاه محقق اردبیلی، اردبیل، ایران.

چکیده

لیپواکسیژنازها (LOX) دی‌اکسیژنازهای دارای آهن غیرهم بوده و در مسیر آپوپتوتید (مرگ برنامه‌ریزی‌شده سلول)، پاسخ گیاهان به تنش‌های زیستی و غیرزیستی نقش دارند. در این مطالعه 95 ژن همولوگ LOX در چهار گونه از خانواده بقولات (نخود، سویا، لوبیا و یونجه یکساله) شناسایی شد که این ژن‌ها بر اساس روابط فیلوژنتیکی با آرابیدوپسیس، برنج، ارزن و جو به زیرگروه‌های 9-LOX، 13-LOX تیپ یک و 13-LOX تیپ دو تقسیم می‌شوند. ژن‌های LOX به‌صورت غیریکنواخت بر روی کروموزوم‌ها پخش شده‌اند. آنزیم‌های کد شده به‌وسیله این ژن‌ها در سیتوپلاسم و کلروپلاست فعال هستند. این ژن‌ها غنی از اینترون دارای شش‌تا نه اینترون بوده و دارای ساختار ژنی و فاز اینترونی حفاظت‌شده هستند. ژن‌های LOX مورد مطالعه دارای دارای دمین‌های حفاظت‌شده لیپواکسیژناز و PLAT/LH2 هستند. وجود چندین عنصر تنظیمی سیس پاسخ به هورمون‌ها و تنش‌ها نظیر ERE، MYB و MYC در پروموتر ژن‌های LOX نشان‌دهنده نقش این ژن‌ها در نمو گیاه و پاسخ آن‌ها به تنش‌های محیطی می‌باشد. افزون بر این، انواع مولکول‌های miRNA شناسایی‌شده بیان پس از رونویسی ژن‌های LOX را از طریق برش یا ممانعت از ترجمه تنظیم می‌کند. آنالیز بیان ژن‌ها بر پایه داده‌های ترانسکریپتومیکس نشان داد که الگوی بیان ژن‌های LOX سویا در شرایط تنش‌های غیرزیستی متفاوت بوده و ژن‌های GmLOX4، GmLOX21، GmLOX25، GmLOX5، GmLOX22، GmLOX24، GmLOX14، GmLOX16، GmLOX7 و GmLOX26 بیان بالایی در پاسخ به شوری، خشکی، سرما و گرما دارند که بر نقش آن‌ها در افزایش تحمل سویا به تنش‌های غیرزیستی دلالت دارد. این مطالعه اطلاعات ارزشمندی را برای درک بهتر کارکردهای ژن‌های LOX و کشفیات بیشتر خانواده ژنی LOX بقولات فراهم می‌کند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Genome-wide identification and functional analysis of lipoxygenase (LOX) gene family in some Fabaceae species using bioinformatics methods

نویسندگان [English]

  • Solmaz Azizi 1
  • Nasser Zare 2
1 Ph.D. Student, Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabil, Iran
2 Professor, Department of Plant Production and Genetics, University of Mohaghegh Ardabili, Ardabil, Iran.
چکیده [English]

Lipoxygenases (LOXs) are non-heme iron-containing dioxygenases involved in the apoptotic (programmed cell death) pathway and biotic and abiotic stress responses in plants. In the present study, we identified 95 LOX homologous genes from four Fabaceae species (Cicer arietinum, Glycine max, Phaseolus vulgaris, Medicago truncatula), which could be divided into 9-LOX, 13-LOX type I, and type II subgroups according to their phylogenetic relationships with Arabidopsis, rice, barley, and foxtail millet. LOX genes are distributed unevenly across the chromosomes, and their coding enzyme is active in the cytoplasm and chloroplast. These genes are intron rich, have six to nine introns, and are conserved in gene structure and intron phase. All identified genes have the conserved lipoxygenase and PLAT/LH2 domains. Several cis-acting elements related to hormones and stresses, such as ERE, MYB, and MYC in the LOXs promoters, indicated the role of these genes in plant development and responses to environmental stresses. In addition, different miRNA molecules were identified that regulate the post-transcriptional expression of LOXs genes through cleavage or inhibition of translation. Transcriptome data-based gene expression analysis showed that Glycine max LOXs expression pattern differed under abiotic stress conditions, and GmLOX4, GmLOX21, GmLOX25, GmLOX5, GmLOX22, GmLOX24, GmLOX14, GmLOX16, GmLOX7, and GmLOX26 were highly expressed in response to salt, drought, cold and heat stresses, indicating that they can improve the tolerance of Glycine max to abiotic stress. This study provides valuable information for a better understanding of the function of LOX genes and further exploration of the LOX gene family in Fabaceae.

کلیدواژه‌ها [English]

  • Gene Expression
  • Evolution
  • In Silico analysis
  • Promoter
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