بررسی ویژگی‌ها و تغییرات بیانی اعضای خانواده ژنی اسنیکین در مراحل مختلف نمو بذر در گیاه جو (Hordeum vulgare L.)

نوع مقاله : علمی پژوهشی

نویسندگان

1 گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه لرستان، خرم‌آباد، ایران.

2 استاد، گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه لرستان، خرم‌آباد، ایران.

3 گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، اهواز، ایران.

چکیده

پپتید‌های ضد میکروبی جزئی از سیستم دفاعی در گیاهان هستند. آنها در تمامی بافت‌ها و در گونه‌های گیاهی مختلفی وجود دارند و دارای اثر ضد میکروبی در برابر پاتوژن‌های گیاهی و جانوری و خواص ضد سرطانی هستند. اسنیکین‌ها گروهی از این پپتیدهای ضد میکروبی غنی از سیستئین با وزن مولکولی پایین هستند که در تنش‌های محیطی، مسیر‌های پیام‌دهی هورمون‌ها و رشد و تکامل نقش دارند. در مطالعه حاضر، با استفاده از روش‌های آزمایشگاهی و بیوانفورماتیکی ویژگی‌های اعضای خانواده ژنی اسنیکین و تغییرات بیانی آنها در مراحل نموی بذر (3، 8، 13 و 18 روز پس از گرده‌افشانی) در گیاه جو مورد بررسی قرار گرفت. نتایج وجود تعداد یازده ژن اسنیکین در ژنوم گیاه جو را نشان داد. اسنیکین‌های شناسایی شده حاوی دمین عملکردی تحریک شونده با جیبرلین بودند. این اسنیکین‌ها دارای سیگنال پپتید و تجمع خارج سلولی بودند و به دلیل فراوانی بالای اسیدهای آمینه هیدروفوب، آبگریز بوده و ساختارهای ثانویه پیچیده‌ای تولید می‌کردند. در همه پروتئین‌های شناسایی شده، وجود تعداد شش پیوند‌ دی‌سولفیدی و خاصیت ضد میکروبی به صورت محاسباتی مشخص گردید. بررسی تغییرات بیانی اعضای خانواده ژنی اسنیکین در مراحل مختلف نمو بذر نشان داد که ژن‌های مذکور دارای الگوهای بیانی کاملاً متفاوتی هستند و در هر مرحله‌ی نموی میزان بیان آن‌ها روند متفاوتی را نشان می‌دهد. با شناسایی ژن‌های اسنیکین، علاوه بر استفاده از آن در تولید گیاهان تراریخت، می‌توان آن را در سیستم‌های مختلف بیانی تولید کرد و به عنوان نسل جدیدی از عوامل آنتی‌بیوتیک طبیعی در محافظت از انسان، گیاهان و جانوران به کار برد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Investigation of the characteristics and expression changes of snakin gene family members in different stages of seed development in barley (Hordeum vulgare L.)

نویسندگان [English]

  • Anahita Panji 1
  • Ahmad Ismaili 2
  • Seyyed Mohsen Sohrabi 3
1 Department of Plant Production and Genetic Engineering, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.
2 Professor, Department of Plant Production and Genetic Engineering, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.
3 Department of Production Engineering and Plant Genetics, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
چکیده [English]

Antimicrobial peptides are a part of the innate immune system in plants. They are present in all tissues and a wide range of plant species, and their antimicrobial effect against plant and animal pathogens and cancer cells has been proven. Snakins are a group of low molecular weight cysteine-rich plant antimicrobial peptides involved in the defense against biotic and abiotic stresses, hormone pathways, and plant growth and development. In the present study, laboratory and bioinformatic methods were used to investigate the characteristics of the snakin gene family members and to evaluate their expression changes in four seed development stages (3, 8, 13, and 18 days after pollination) in barley plants. The results showed the presence of 11 snakin genes in the genome of barley. The protein sequences of the identified snakins contained the GASA functional domain. These snakins had a signal peptide and had extracellular accumulation. Due to their high abundance of hydrophobic amino acids, they were hydrophobic and produced complex secondary structures. Phylogenetic analysis was performed between barley, rice, and arabidopsis snakins as two monocot and dicot models, leading to three classes. Also, six disulfide bonds and antimicrobial properties were computationally confirmed in all identified proteins. Expression analysis showed different expression patterns for snakin gene family members in different stages of seed development and also exhibited different trends in each stage. The snakin genes can use to produce transgenic plants and to produce a new generation of natural antibiotic agents to protect humans, plants, and animals.

کلیدواژه‌ها [English]

  • Antimicrobial activity
  • Bioinformatics analysis
  • Seed development stages
  • Gene expression trend
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