نوع مقاله : علمی پژوهشی
نویسندگان
1 عضو علمی دانشگاه پیام نور
2 عضو هیئت علمی
3 پژوهشگاه ملی مهندسی ژنتیک
چکیده
Culture medium, Embryo culture, Taxol, Yew,
کلیدواژهها
موضوعات
عنوان مقاله [English]
Shortening the germination time of Iranian yew (Taxus baccata sp.) through embryo culture
نویسندگان [English]
- Hooshang Goharchini 1
- khadijeh bagheri 2
- Mohammad Reza Zamani 3
1 Scientific member of Payam Noor University
2 Department Genetics and plant products.agriculture faculty, zanjan university, zanjan.Iran
3 Prof. of Noor Danesh Institute of Higher Education, Mimeh, Isfahan, Iran
چکیده [English]
Yew plant is the main source of Taxol drug production. Paclitaxol with the brand name Taxol is the main drug in the treatment of various cancers. Due to the long dormancy of the seeds, difficult germination and indiscriminate harvesting in some areas, there is a risk of extinction of this very important plant. According to these conditions, it is necessary to pay attention to biotechnology tools to produce new seedlings for propagation as well as use in other in vitro studies. In this research, in the form of a completely randomized experiment design with 48 treatments and three repetitions, it was determined that the highest germination was observed in the treatment of 21 days of keeping the seeds in water and growing them on ½ WPM culture medium. In the next step, in order to shorten the germination time, the embryos were isolated in a sterile environment and kept in sterile double distilled water on a refrigerated shaker for 5 days at a temperature of 4°C. Variance analysis revealed that there is no significant difference between treatments in germination speed, so it can be concluded that young and fresh seedlings can be obtained in less time with this method.
کلیدواژهها [English]
- Culture medium
- Embryo culture
- Taxol
- Yew
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