شناسایی نوروپپتید‌‌های خانواده آلاتواستاتین و گیرنده‌های نوروپپتیدی آن در سن گندم (Eurygaster integriceps) با استفاده از راهکار کاوش ترانسکریپتوم

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی دکتری بیوتکنولوژی کشاورزی، گروه ژنتیک و تولیدات گیاهی، دانشکده کشاورزی دانشگاه کردستان، سنندج، ایران. و بخش زیست‌شناسی سامانه‌ها، پژوهشگاه بیوتکنولوژی کشاورزی، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران.

2 دانشیار گروه ژنتیک و تولیدات گیاهی، دانشکده کشاورزی دانشگاه کردستان، سنندج، ایران.

3 استاد گروه علوم مولکولی، دانشگاه مک کواری، نورث راید، ملبورن، استرالیا.

4 بخش زیست‌شناسی سامانه‌ها، پژوهشگاه بیوتکنولوژی کشاورزی، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران.

چکیده

سن گندم با نام علمی Eurygaster integriceps یکی از آفات شناخته شده مزارع گندم در ایران و غرب آسیا است. نقش نوروپپتید‌‌ها در مراحل رشد و نمو حشرات منجر به ‌ایجاد چشم‌انداز امیدوارکننده‌ای جهت تولید نسل جدیدی از حشره‌کش‌ها‌ی زیستی مبتنی بر کاربرد اختصاصی شده است. نوروپپتیدهای حشرات به همراه گیرنده‌های اختصاصی آن‌ها یکی از متنوع‌ترین پروتئین‌هایی هستند که فعالیت‌های فیزیولوژیکی و رفتاری را در حشرات کنترل می‌کنند. آلاتواستاتین یکی از نوروپپتیدهای مهم در حشرات می‌باشد که با مهار هورمون جوانی، در تنظیم فرآیندهای فیزیولوژیکی نظیر تغذیه و متابولیسم در برخی از حشرات نقش دارد. در این مطالعه با استفاده از اطلاعات حاصل از ترانسکریپتوم حشره بالغ سن گندم، نوروپپتید‌ها و گیرنده‌های اختصاصی خانواده آلاتواستاتین در سن گندم مورد بررسی قرار گرفت. آنالیز بیوانفورماتیکی و بررسی‌های فیلوژنتیکی داده‌ها منجر به شناسایی چهار نوروپپتید از خانواده آلاتواستاتین A، B و C و همچنین گیرنده‌های نوروپپتیدی آلاتواستاتین A و B شد. نتایج نشان داد که نوروپپتیدهای خانواده آلاتواستاتین شناسایی شده سن گندم در فرایندهای فیزیولوژیک متنوعی دخیل می‌باشند. با توجه به نقش مهم نوروپپتیدها در حشرات، این نوروپپتیدها می‌توانند امکان طراحی حشره‌کش‌های اختصاصی سازگار با محیط زیست به منظور مدیریت کنترل سن گندم را برای آینده فراهم آورند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Identification of allatostatin neuropeptides family and their receptors in Sunn pest (Eurygaster integriceps) using transcriptome wide analysis

نویسندگان [English]

  • Mehrbanno Kazemi Alamuti 1
  • Mohammad Majdi 2
  • Ghasem Hosseini Salekdeh 3
  • Mohammad Reza Ghaffari 4
1 Ph.D. Student, Department of Plant Genetics and Production, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran. and Department of Systems and Synthetic Biology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
2 Department of Systems and Synthetic Biology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
3 Prof. Department of Molecular Sciences, Macquarie University, North Ryde, NSW. Australia.
4 Department of Systems and Synthetic Biology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
چکیده [English]

Eurygaster integriceps is one of the known pests of wheat fields in Iran and West Asia. The role of neuropeptides in the stages of insect’s growth has led to a promising perspective for the production of a new generation of bio-insecticides based specific application. Insect’s neuropeptides along with their specific receptors are one of the most diverse proteins that control physiological and behavioral activities in insects. Allatostatin is one of the important neuropeptides in insects which, by inhibiting the youth hormone, plays a role in regulating physiological processes such as feed and metabolism in some insects. In this study, using the information obtained from adult Eurygaster integriceps transcriptome, neuropeptides and specific receptors of the allatostatin family were investigated. Bioinformatics and phylogenetic studies of the data led to identify four neuropeptides A, B and C allatostatin family, as well as the neuropeptide receptors of A and B allatostatin. The results showed that the neuropeptides of the allatostatin family identified in Eurygaster integriceps are involved in various physiological processes. Considering the important role of neuropeptides in insects, these neuropeptides can be used to design specific insecticides compatible with the environment for managing control Eurygaster integriceps in future.

کلیدواژه‌ها [English]

  • Allatostatin
  • GPCR
  • Neuropeptide
  • RNA-Seq
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