بررسی تغییرات بیوشیمیایی و مورفوفیزیولوژیک برخی ارقام فلفل آلوده به ویروس وای سیب ‏زمینی (‏PVY‏)‏

نوع مقاله : علمی پژوهشی

نویسندگان

1 'گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

3 دانشیار گروه گیاه‌پزشکی، دانشکده تولیدات گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گلستان

4 دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

5 بخش تحقیقات گیاهپزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان گلستان، سازمان تحقیقات، آموزش و ترویج کشاورزی، گرگان، ایران.

6 دانشیار گروه اصلاح نباتات و بیوتکنولوژی دانشگاه علوم کشاورزی و منابع طبیعی گرگان

چکیده

ویروس وای سیب‌زمینی (PVY) یکی از مهمترین و شایع‌ترین بیماری‌های ویروسی خانواده Solanaceae است و مؤثرترین روش مبارزه با آن استفاده از ارقام مقاوم است. در این پژوهش واکنش هفت رقم فلفل به صورت فاکتوریل بر پایه طرح کاملاً تصادفی با چهار تکرار در دو تیمار شاهد و آلودگی با ویروس PVY بررسی شد. نتایج تیمارهای مایه‌زنی نشان دادند شاخص‌های رشدی شامل ارتفاع بوته (84/31 درصد)، وزن‌تر و خشک اندام هوایی (55/46 و 61/46 درصد)، وزن‌تر و خشک کل بوته (16/50 و 09/50 درصد)، وزن‌تر و خشک ریشه (93/51 و 76/54 درصد) در ارقام مایه‌زنی شده به PVY نسبت به شاهد کاهش معنی‌دار نشان داد، در حالی‌که میزان کلروفیل‌a، b و کل، کاروتنوئید، محتوی فنول، فلاونوئید و شدت آلودگی تمامی ارقام مورد مطالعه نسب به شاهد افزایش نشان داد. جهت تأیید آلودگی گیاهان مایه زنی شده، شناسایی ویروسPVY با آزمون الایزای مستقیم و با آنتی سرم IgG- PVY و آزمون RT-PCR با استفاده از آغازگرهای اختصاصی PVY-8687F وPVY-9295R انجام شد. نتایج آزمـون RT-PCR نشان‌دهنده تکثیر قطعه‌ای به اندازه 327 جفت باز بود که بیانگر تایید آزمون الایزا بود و با نتایج تعیین توالی در پایگاه داده NCBI (Accession number–KR909091.1) که قبلا برای این جدایه ثبت شده بود مطابقت داشت. در مجموع رقم کائوبا عکس العمل بهتری در مواجه با آلودگی ویروسی نشان داد، لذا می‌توان این رقم را برای مطالعات آتی به‌نژادی ارقام فلفل پیشنهاد نمود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Assessment of biochemical and morphophysiological changes of some pepper cultivars ‎infected with Potato Virus Y (PVY)‎

نویسندگان [English]

  • Samane Vatani 1
  • Saeed Nasrollanejad 2
  • Abdolhossein Taheri 3
  • Farzad Aliramaji 4
  • Sharbano Vakili 5
  • Leila Fahmideh 6
1 Gorgan University of Agricultural Sciences and Natural Resources Plant Production Faculty
2 Department of Plant Protection, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
3 Associate Professor of Plant Protection, Plant protection department, Gorgan University of Agricultural Sciences and Natural Resources, Golestan
4 Gorgan University of Agricultural Sciences and Natural Resources Plant Production Faculty
5 Plant Protection Research Department, Agriculture and Natural Resources Research and Education Center of Golestan Province, Agricultural Research, Education and Extension Organization, Gorgan, Iran
6 Department of Plant Breeding and Biotechnology Gorgan University of Agricultural Sciences and Natural Resources
چکیده [English]

Potato virus Y (PVY) is one of the most important and widely distributed viral diseases of Solanaceae family and the use of resistant cultivars is the most effective control measure of this disease. In this research, the response of seven pepper cultivars was investigated in two conditions, healthy control and infection with PVY. This experiment was performed as a factorial in a completely randomized design with four replications. The results showed that under the conditions of viral infection, the growth indices including: height of the plant (31.84%), shoot fresh and dry weight (46.55 and 46.61%), plant fresh and dry weight (50.16 and 50.09%), root fresh and dry weight (54.76 and 51.93%) of all seven cultivars decreased compared to the ‎control conditions.‎While the amount of chlorophyll a, b and total chlorophyll, carotenoid, the content of phenol and flavonoid and diseases severity of all studied cultivars increased compared to the control conditions. To confirm the infection of treated plants, PVY virus identification was performed by direct ELISA test with IgG-PVY antiserum and RT-PCR test using specific primers PVY-8687F and PVY-9295R.The results of the RT-PCR test showed the amplification of a fragment of 327 base pairs, which indicates the confirmation of the ELISA test and it was consistent with the sequence determination results in the NCBI database (Accession number–KR909091.1) that was previously registered for this isolate. In general, the Cauba cultivar has shown a better reaction in the face of infection with PVY virus. So, this cultivar can be suggested for future studies of pepper cultivars.

کلیدواژه‌ها [English]

  • Pepper cultivars
  • Solanaceae family
  • Photosynthetic pigments
  • Growth traits
  • Phenol and flavonoid content
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