با همکاری مشترک دانشگاه پیام نور و انجمن بیوتکنولوژی جمهوری اسلامی ایران

نوع مقاله : علمی پژوهشی

نویسندگان

1 دکتری اصلاح نباتات، واحد اراک، دانشگاه آزاد اسلامی اراک، اراک، ایران.

2 استادیار، گروه زراعت و اصلاح نباتات، واحد اراک، دانشگاه آزاد اسلامی اراک، اراک، ایران.

3 استاد، مؤسسه تحقیقات جنگل‌ها و مراتع کشور، بخش بیوتکنولوژی گیاهی، تهران.

4 استادیار گروه زراعت و اصلاح نباتات، واحد اراک، دانشگاه آزاد اسلامی، اراک، ایران

5 دانشیار گروه زراعت و اصلاح نباتات، واحد اراک، دانشگاه آزاد اسلامی، اراک، ایران

چکیده

از طریق کشت ریزنمونه مریستم انتهایی و ایجاد تنوع سوماکلونال ژنوتیپ امیدبخش از رقم سیب‌زمینی آگریا بر اساس رشد بیشتر کالوس در محیط کشت و همچنین ریشه‌زایی و ساقه‌زایی بیشتر که با تولید برگ و سرعت رشد بیشتری نیز همراه بود انتخاب شد. ژنوتیپ جدید همراه با رقم والدی در یک آزمایش مزرعه‌ای در قالب طرح بلوک‌های کامل تصادفی با سه تکرار برای تعدادی از صفات مورفولوژیک مورد ارزیابی قرار گرفت. نتایج نشان داد که صفات میزان ماده خشک (%5/13)، تعداد (%3/12) و وزن (%6/8) غده در هر بوته و طول استولون (%4) در ژنوتیپ امیدبخش نسبت به رقم آگریا به‌طور معنی‌داری بیشتر و همچنین صفت روز تا 50 درصد گلدهی (%4/11) و زمان رسیدن (%6/7) در این ژنوتیپ به‌طور معنی داری کمتر از رقم والدی بود. برخی از خصوصیات تغذیه‌ای نیز در دو ژنوتیپ در قالب طرح کاملاً تصادفی با 3 تکرار ارزیابی شد. نتایج نشان‌دهنده آن بود که فعالیت آنتی‌اکسیدانی قابل توجهی در ژنوتیپ جدید (% 6/35) در مقایسه با رقم والدی (% 3/25) وجود داشت. به علاوه، ژنوتیپ تولید شده به‌طور معنی‌داری حاوی مقدار نیتریت کمتر (%2/81) و مقدار آهن (%7/20) و نشاسته (%2/21) بیشتری بود. بنابراین، به نظر می‌رسد رقم حاصل واجد پتانسیل‌های قابل قبول جهت کشت و جایگزینی با رقم‌های موجود است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Assessment some morphological and nutritional characteristics of a promising potato genotype, produced from somaclonal variation, compared with its parental cultivar

نویسندگان [English]

  • Eshaghali Bayati 1
  • Masoud Gomarian 2
  • Hossein Mirzaie Nodoushan 3
  • Mahdi Changizi 4
  • Shahab Khaghani 5

1 Ph.D. Plant Breeding, Department of Agronomy and Plant Breeding, Arak Branch, Islamic Azad University, Arak, Iran.

2 Assistant Professor, Department of Agronomy and Plant Breeding, Arak Branch, Islamic Azad University, Arak, Iran.

3 Professor, Research Institute of Forests and Rangelands, Tehran, I.R. Iran.

4 Assistant Professor, Department of Agronomy and Plant Breeding, Arak Branch, Islamic Azad University, Arak, Iran

5 Associate Professor, Department of Agronomy and Plant Breeding, Arak Branch, Islamic Azad University, Arak, Iran

چکیده [English]

In a laboratory experiment conducted in Kabudarahang, Hamadan, a promising potato genotype was selected through somaclonal variation from Agria commercial cultivar, based on the callus size on growth media, shoot and root regeneration. A field experimental design with three replications was carried out to assess the new genotype along with its parental commercial cultivar for several morphological traits. Results revealed that total dry matter per plant (13.5%), total number of tubers (12.3%), tuber weight per plant (8.6%), and stolon length (4%) were significantly higher than in the new genotype. Also, days to 50% flowering (11.4%) and maturity date (7.6%) were significantly less in comparison with the parental cultivar. Several nutritional traits were also studied by which more antioxidant activity was observed on the new genotype (35.6%) than Agria cultivar (25.3%). Furthermore, the new genotype had a lower nitrite (81.2%) and higher iron (20.7%) and starch content (21.2%) comparing with Agria cultivar. Therefore, it seems that the new genotype has an acceptable potential for cultivation and replacement with existing cultivars.

کلیدواژه‌ها [English]

  • Antioxidant
  • Somaclonal variation
  • Maturity date
  • Promising genotype
  • Nitrite
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